Asic2 (NM_001034014) Rat Tagged ORF Clone

CAT#: RR200428

Asic2 (Myc-DDK-tagged ORF) - Rat amiloride-sensitive cation channel 1, neuronal (Accn1), transcript variant 2, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Asic2
• Synonyms: Accn1; BNaC1; BNC1k; MDEG; MDEG1; MDEG2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200428 representing NM_001034014
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCTCAAGGAGAGCCCCAGTGAGGGCAGCCTGCAACCTTCCAGTATCCAGATCTTCGCCAATACCT
  CCACTCTCCATGGCATCCGCCACATCTTCGTGTATGGGCCGCTGACCATCCGGCGTGTGCTTTGGGCAGT
  GGCCTTCGTCGGATCCCTGGGCCTGCTGCTGGTGGAGAGCTCGGAGAGGGTGTCCTACTATTTCTCTTAT
  CAGCATGTTACCAAGGTGGATGAAGTGGTGGCCCAGAGCCTGGTCTTCCCAGCTGTGACCCTCTGCAACC
  TCAATGGCTTCCGGTTCTCCAGGCTTACCACCAACGACTTGTACCACGCTGGGGAGTTGCTGGCCCTGCT
  GGATGTCAACCTACAGATTCCCGACCCGCATCTGGCAGACCCCACGGTGCTGGAGGCCCTCCGACAGAAG
  GCCAACTTCAAACACTACAAACCGAAGCAGTTCAGCATGCTGGAGTTCCTGCACCGGGTAGGCCATGACC
  TGAAGGATATGATGCTCTACTGCAAGTTCAAGGGGCAGGAGTGTGGGCATCAAGACTTCACCACAGTGTT
  TACAAAATACGGGAAGTGTTACATGTTTAACTCAGGCGAGGATGGCAAGCCGCTGCTCACCACGGTCAAG
  GGGGGGACGGGCAACGGGCTGGAGATCATGCTGGACATTCAGCAAGATGAGTACCTGCCCATCTGGGGAG
  AGACAGAGGAAACAACGTTTGAAGCAGGAGTGAAGGTTCAGATCCACAGTCAGTCTGAGCCGCCTTTCAT
  CCAAGAGCTGGGCTTTGGGGTGGCTCCGGGGTTCCAGACCTTCGTGGCCACACAAGAGCAGAGGCTCACA
  TATCTGCCCCCACCATGGGGGGAGTGCCGGTCCTCAGAGATGGGACTCGACTTCTTTCCTGTTTACAGCA
  TCACAGCCTGTCGGATTGACTGTGAGACCCGCTACATCGTGGAGAACTGTAACTGCCGCATGGTCCACAT
  GCCAGGGGACGCCCCTTTCTGCACCCCTGAGCAGCACAAGGAGTGTGCAGAGCCTGCCCTCGGTCTACTG
  GCAGAAAAGGACAGCAATTACTGTCTCTGCAGGACACCCTGCAACCTGACACGCTACAACAAAGAGCTCT
  CCATGGTGAAGATCCCCAGCAAGACGTCAGCCAAGTACTTAGAGAAGAAATTTAACAAATCGGAAAAATA
  TATCTCAGAGAACATTCTTGTTCTGGACATATTTTTTGAGGCGCTCAATTACGAAACAATTGAACAGAAG
  AAGGCGTATGAAGTTGCTGCCTTACTTGGTGACATCGGTGGTCAGATGGGACTGTTCATTGGTGCTAGTC
  TCCTCACAATACTAGAGCTCTTTGATTATATTTATGAGCTGATCAAAGAGAAGCTATTAGACCTGCTTGG
  CAAAGAAGAAGAGGAAGGGAGCCACGATGAGAACATGAGCACCTGTGACACAATGCCAAACCACTCTGAA
  ACCATCAGCCACACTGTGAACGTGCCCCTGCAGACAGCTTTGGGCACCCTGGAGGAGATTGCCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200428 representing NM_001034014
  Red=Cloning site Green=Tags(s)
  
  MDLKESPSEGSLQPSSIQIFANTSTLHGIRHIFVYGPLTIRRVLWAVAFVGSLGLLLVESSERVSYYFSY
  QHVTKVDEVVAQSLVFPAVTLCNLNGFRFSRLTTNDLYHAGELLALLDVNLQIPDPHLADPTVLEALRQK
  ANFKHYKPKQFSMLEFLHRVGHDLKDMMLYCKFKGQECGHQDFTTVFTKYGKCYMFNSGEDGKPLLTTVK
  GGTGNGLEIMLDIQQDEYLPIWGETEETTFEAGVKVQIHSQSEPPFIQELGFGVAPGFQTFVATQEQRLT
  YLPPPWGECRSSEMGLDFFPVYSITACRIDCETRYIVENCNCRMVHMPGDAPFCTPEQHKECAEPALGLL
  AEKDSNYCLCRTPCNLTRYNKELSMVKIPSKTSAKYLEKKFNKSEKYISENILVLDIFFEALNYETIEQK
  KAYEVAALLGDIGGQMGLFIGASLLTILELFDYIYELIKEKLLDLLGKEEEEGSHDENMSTCDTMPNHSE
  TISHTVNVPLQTALGTLEEIAC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034014
• ORF Size: 1536 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034014.1, NP_001029186.1
• RefSeq Size: 2565 bp
• RefSeq ORF: 1539 bp
• Locus ID: 25364
• Cytogenetics: 10q26
• MW: 57.7 kDa
• Gene Summary: member of the degenerin/epithelial Na(+) channel superfamily; permeable for Na(+), K(+) and Li(+); mutations may lead to a form of hereditary neurodegeneration; may be important for pain sensation [RGD, Feb 2006]