Dclk1 (NM_053343) Rat Untagged Clone

CAT#: RN200772

Dclk1 (untagged ORF) - Rat doublecortin-like kinase 1 (Dclk1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Dclk1
• Synonyms: Ania4; Cpg16; Dcamkl1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200772 representing NM_053343
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTAGAACTCATAGAAGTTAATGGAACCCCTGGCAGTCAGCTCTCCACTCCGCGCTCCGGCAAGTCAC
  CAAGTCCATCGCCCACCAGCCCAGGAAGCCTGCGGAAGCAGAGGATCTCTCAGCATGGCGGCTCCTCCAC
  TTCACTTTCATCCACAAAAGTCTGCAGCTCAATGGATGAGAACGATGGCCCTGGGGAAGAAGAGTCCGAC
  GAAGGTTTCCAGATTCCTGCCACAATAACAGAGAGATACAAAGTCGGGAGAACAATAGGAGATGGAAATT
  TCGCTGTTGTCAAGGAATGTATAGAGAGGTCGACTGCTCGGGAGTATGCCCTGAAAATTATCAAGAAAAG
  TAAATGCCGAGGCAAAGAACACATGATCCAAAACGAGGTCTCCATCTTAAGGAGAGTGAAGCATCCGAAC
  ATTGTTCTCCTGATTGAAGAGATGGATGTGCCGACTGAACTATATCTTGTAATGGAGTTAGTAAAGGGTG
  GAGACCTTTTCGATGCCATCACTTCCACTAGCAAATACACAGAGCGGGATGCCAGTGGGATGCTGTACAA
  CCTGGCCAGTGCCATTAAATACCTGCACAGCCTGAACATCGTCCATCGTGACATCAAGCCAGAGAACCTG
  CTGGTGTATGAGCACCAGGATGGCAGTAAGTCACTCAAGTTGGGTGACTTTGGCCTGGCCACAATTGTCG
  ATGGCCCCCTGTACACAGTCTGTGGCACCCCAACTTATGTGGCTCCAGAAATCATTGCAGAGACTGGATA
  TGGCCTCAAGGTGGACATCTGGGCAGCTGGCGTGATCACTTATATCCTGTTGTGTGGTTTCCCTCCATTC
  CGTGGAAGTGGGGATGACCAGGAGGTGCTTTTTGACCAGATCTTGATGGGCCAAGTGGACTTTCCATCTC
  CGTATTGGGACAATGTGTCAGATTCTGCTAAGGAGCTCATCAACATGATGCTGTTGGTTAACGTGGACCA
  GAGATTTTCAGCCGTGCAAGTCCTTGAGCACCCCTGGGTTAACGATGATGGCCTCCCAGAAAATGAACAT
  CAGCTGTCAGTAGCTGGCAAGATCAAGAAGCATTTCAACACGGGCCCCAAGCCGAGCAGCACGGCAGCTG
  GAGTTTCTGTAATAGCAACCACCGCTCTTGATAAGGAGAGGCAGGTTTTCCGACGAAGACGCAACCAGGA
  TGTGAGGGGCCGGTACAAGGCACAGCCAGCTCCACCAGAACTCAACTCGGAATCAGAAGACTACTCCCCC
  AGCTCCTCTGAGACTGTCCGCTCCCCCAATTCGCCCTTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053343
• ORF Size: 1302 bp
• Insert Size: 1302
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_053343.3, NP_445795.1
• RefSeq Size: 6903
• RefSeq ORF: 1302
• Locus ID: 83825
• Gene Summary: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The typical protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the protein is independent of its protein kinase activity. This gene is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been found, but the full-length nature of the variant produced from the 5' promoter has not been determined. Current reference sequence data represents two alternatively spliced transcript variants produced from the 3' promoter and their protein products lack the doublecortin domain. [provided by RefSeq, Sep 2010]
  Transcript Variant: This variant [1, also known as DCLK-short-A, DCK-beta2, KIAA0369-BL or cpg16 (candidate plasticity gene 16)] is produced from the 3' promoter. It encodes isoform 1, which contains a serine/proline-rich domain and a protein kinase domain. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.