Dclk1 (NM_053343) Rat Tagged ORF Clone

CAT#: RR200772

Dclk1 (Myc-DDK-tagged ORF) - Rat doublecortin-like kinase 1 (Dclk1), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dclk1
• Synonyms: Ania4; Cpg16; Dcamkl1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200772 representing NM_053343
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTAGAACTCATAGAAGTTAATGGAACCCCTGGCAGTCAGCTCTCCACTCCGCGCTCCGGCAAGTCAC
  CAAGTCCATCGCCCACCAGCCCAGGAAGCCTGCGGAAGCAGAGGATCTCTCAGCATGGCGGCTCCTCCAC
  TTCACTTTCATCCACAAAAGTCTGCAGCTCAATGGATGAGAACGATGGCCCTGGGGAAGAAGAGTCCGAC
  GAAGGTTTCCAGATTCCTGCCACAATAACAGAGAGATACAAAGTCGGGAGAACAATAGGAGATGGAAATT
  TCGCTGTTGTCAAGGAATGTATAGAGAGGTCGACTGCTCGGGAGTATGCCCTGAAAATTATCAAGAAAAG
  TAAATGCCGAGGCAAAGAACACATGATCCAAAACGAGGTCTCCATCTTAAGGAGAGTGAAGCATCCGAAC
  ATTGTTCTCCTGATTGAAGAGATGGATGTGCCGACTGAACTATATCTTGTAATGGAGTTAGTAAAGGGTG
  GAGACCTTTTCGATGCCATCACTTCCACTAGCAAATACACAGAGCGGGATGCCAGTGGGATGCTGTACAA
  CCTGGCCAGTGCCATTAAATACCTGCACAGCCTGAACATCGTCCATCGTGACATCAAGCCAGAGAACCTG
  CTGGTGTATGAGCACCAGGATGGCAGTAAGTCACTCAAGTTGGGTGACTTTGGCCTGGCCACAATTGTCG
  ATGGCCCCCTGTACACAGTCTGTGGCACCCCAACTTATGTGGCTCCAGAAATCATTGCAGAGACTGGATA
  TGGCCTCAAGGTGGACATCTGGGCAGCTGGCGTGATCACTTATATCCTGTTGTGTGGTTTCCCTCCATTC
  CGTGGAAGTGGGGATGACCAGGAGGTGCTTTTTGACCAGATCTTGATGGGCCAAGTGGACTTTCCATCTC
  CGTATTGGGACAATGTGTCAGATTCTGCTAAGGAGCTCATCAACATGATGCTGTTGGTTAACGTGGACCA
  GAGATTTTCAGCCGTGCAAGTCCTTGAGCACCCCTGGGTTAACGATGATGGCCTCCCAGAAAATGAACAT
  CAGCTGTCAGTAGCTGGCAAGATCAAGAAGCATTTCAACACGGGCCCCAAGCCGAGCAGCACGGCAGCTG
  GAGTTTCTGTAATAGCAACCACCGCTCTTGATAAGGAGAGGCAGGTTTTCCGACGAAGACGCAACCAGGA
  TGTGAGGGGCCGGTACAAGGCACAGCCAGCTCCACCAGAACTCAACTCGGAATCAGAAGACTACTCCCCC
  AGCTCCTCTGAGACTGTCCGCTCCCCCAATTCGCCCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200772 representing NM_053343
  Red=Cloning site Green=Tags(s)
  
  MLELIEVNGTPGSQLSTPRSGKSPSPSPTSPGSLRKQRISQHGGSSTSLSSTKVCSSMDENDGPGEEESD
  EGFQIPATITERYKVGRTIGDGNFAVVKECIERSTAREYALKIIKKSKCRGKEHMIQNEVSILRRVKHPN
  IVLLIEEMDVPTELYLVMELVKGGDLFDAITSTSKYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENL
  LVYEHQDGSKSLKLGDFGLATIVDGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPF
  RGSGDDQEVLFDQILMGQVDFPSPYWDNVSDSAKELINMMLLVNVDQRFSAVQVLEHPWVNDDGLPENEH
  QLSVAGKIKKHFNTGPKPSSTAAGVSVIATTALDKERQVFRRRRNQDVRGRYKAQPAPPELNSESEDYSP
  SSSETVRSPNSPF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053343
• ORF Size: 1299 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053343.1, NM_053343.2, NM_053343.3, NP_445795.1
• RefSeq Size: 6903
• RefSeq ORF: 1302
• Locus ID: 83825
• MW: 47.7 kDa
• Gene Summary: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The typical protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the protein is independent of its protein kinase activity. This gene is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been found, but the full-length nature of the variant produced from the 5' promoter has not been determined. Current reference sequence data represents two alternatively spliced transcript variants produced from the 3' promoter and their protein products lack the doublecortin domain. [provided by RefSeq, Sep 2010]