Nos3 (NM_021838) Rat Untagged Clone

CAT#: RN200806

Nos3 (untagged ORF) - Rat nitric oxide synthase 3, endothelial cell (Nos3), (10 ug)


  "NM_021838" in other vectors (1)

Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Symbol Nos3
Synonyms eNos
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN200806 representing NM_021838
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAACTTGAAGAGTGTGGGCCAGGAGCCTGGGCCACCCTGTGGCCTAGGGCTCGGGCTGGGCCTAG
GGCTATGCGGCAAGCAGGGCCCAGCCTCACCGGCACCAGAGCCTAGCCAGGCACCAGTACCCCCGTCCCC
AACCCGACCAGCACCAGACCACAGCCCCCCGTTAACCCGGCCCCCAGACGGACCCAAGTTTCCTCGAGTA
AAGAACTGGGAAGTGGGCAGCATCACCTACGATACCCTCAGTGCACAGGCTCAGCAGGATGGGCCCTGTA
CCCCAAGACGCTGCTTGGGATCCCTGGTATTTCCAAGGAAGTTACAGAGCCGGCCCACCCAGGGCCCTTC
ACCCACTGAGCAGCTATTGGGTCAAGCCCGGGACTTCATCAATCAGTACTATAACTCGATCAAAAGGAGT
GGTTCCCAGGCTCATGAGCAGCGGCTTCAGGAAGTGGAAGCTGAGGTGGTGGCCACGGGCACCTACCAGC
TCCGGGAGAGTGAGCTGGTGTTTGGGGCCAAACAGGCCTGGCGCAACGCTCCCCGCTGTGTGGGGCGGAT
CCAGTGGGGGAAACTGCAGGTATTTGATGCTCGGGACTGCAGGACAGCACAGGAAATGTTCACCTACATC
TGTAACCACATTAAGTATGCAACAAACCGAGGCAATCTTCGTTCAGCCATCACGGTGTTCCCCCAGCGCT
ACGCTGGCCGGGGAGACTTCCGGATCTGGAACAGCCAGCTGGTGCGCTACGCGGGCTATAGGCAGCAGGA
CGGCTCTGTGCGAGGGGACCCTGCCAACGTGGAGATCACTGAGCTCTGTATTCAACATGGCTGGACCCCA
GGAAATGGCCGCTTTGATGTGCTGCCCCTGCTACTCCAGGCTCCCGATGAGCCCCCAGAACTCTTCACTC
TGCCCCCAGAGCTGGTCCTCGAGGTGCCTCTGGAGCACCCCACGCTAGAGTGGTTTGCTGCCCTTGGCCT
GCGCTGGTATGCCCTCCCAGCTGTGTCCAATATGCTGCTAGAAATCGGGGGCCTGGAGTTTCCGGCTGCC
CCTTTCAGCGGCTGGTACATGAGTTCAGAGATTGGCATGAGGGACCTGTGTGACCCTCACCGATACAACA
TACTTGAGGATGTGGCTGTCTGCATGGATCTAGACACCCGGACAACCTCATCACTGTGGAAAGACAAGGC
AGCAGTGGAAATTAACGTGGCTGTGCTGTACAGTTACCAGCTGGCCAAAGTGACCATTGTGGACCACCAT
GCCGCCACAGCCTCCTTCATGAAGCACTTGGAAAATGAGCAGAAGGCCAGAGGGGGCTGCCCCGCTGACT
GGGCCTGGATCGTGCCCCCCATCTCAGGCAGCCTCACCCCTGTCTTCCATCAAGAGATGGTCAACTATTT
CCTGTCCCCTGCCTTCCGCTACCAGCCTGACCCCTGGAAAGGAAGTGCAGCAAAAGGCACAGGCATCACC
AGGAAGAAGACTTTTAAGGAAGTAGCCAATGCAGTGAAGATCTCTGCCTCACTCATGGGCACGGTGATGG
CGAAGCGTGTGAAGGCGACTATCCTGTATGGCTCTGAGACTGGCCGCGCCCAGAGCTACGCACAGCAGCT
GGGGAGGCTCTTTCGGAAGGCGTTTGACCCCCGGGTCCTGTGCATGGATGAATACGATGTGGTATCCCTA
GAGCATGAGGCCTTGGTATTGGTGGTGACCAGCACATTTGGCAATGGGGATCCCCCGGAGAATGGAGAGA
GCTTTGCAGCAGCGCTGATGGAAATGTCGGGCCCCTACAACAGCTCCCCTCGGCCTGAGCAGCACAAGAG
TTACAAAATCCGATTCAACAGTGTCTCCTGCTCGGACCCACTGGTATCCTCTTGGCGGCGCAAAAGGAAG
GAATCCAGTAACACAGACAGTGCAGGGGCCCTGGGCACCCTCAGGTTCTGTGTGTTTGGGCTGGGCTCCA
GAGCATACCCGCACTTCTGTGCCTTTGCTCGAGCGGTGGACACAAGGCTGGAGGAGCTGGGCGGGGAGCG
ACTGTTGCAGCTGGGCCAGGGTGATGAGCTCTGCGGCCAGGAGGAGGCTTTCCGAGGCTGGGCCCAGGCA
GCCTTCCAGGCTGCCTGTGAAACTTTCTGTGTGGGAGAAGATGCCAAGGCTGCTGCCCGAGATATCTTCA
GTCCCAAGCGCAGCTGGAAACGCCAGAGGTACCGGCTGAGTACCCAAGCTGAGAGCCTGCAATTACTGCC
AGGGCTGACTCACGTGCACAGACGGAAGATGTTCCAGGCTACAATTCTTTCTGTGGAGAACCTACAGAGC
AGCAAATCCACCCGAGCCACAATCCTGGTGCGTCTGGACACTGGAAGCCAGGAGGGACTGCAGTACCAGC
CAGGGGACCACATAGGTGTGTGCCCACCCAACCGGCCTGGCCTAGTGGAGGCGCTGCTGAGCCGAGTGGA
GGACCCTCCGCCATCCACAGAGCCTGTGGCCGTGGAACAACTGGAAAAAGGCAGCCCTGGTGGCCCTCCC
CCCGGCTGGGTACGGGACCCCCGGCTTCCCCCATGTACGCTGCGCCAGGCTCTCACTTACTTCCTGGACA
TCACTTCCCCGCCCAGCCCTCGCCTTCTTCGACTGCTGAGCACCCTGGCAGAGGAGTCCAGCGAACAGCA
GGAGCTAGAGGCTCTTAGCCAGGACCCCCGGCGCTACGAAGAATGGAAGTGGTTCCGCTGCCCCACACTG
CTAGAGGTGCTGGAACAATTTCCATCCGTGGCACTGCCTGCCCCGCTGATCCTCACCCAGCTGCCCCTGC
TCCAGCCCCGGTACTACTCTGTCAGCTCAGCACCCAGCGCCCACCCAGGAGAGATCCACCTCACTGTAGC
TGTGCTGGCATACAGAACCCAGGATGTGCTGGGCCCTCTGCACTATGGGGTCTGTTCCACATGGATGAGC
CAACTCAAGGCAGGAGACCCGGTGCCCTGCTTCATCAGGGGGGCTCCCTCCTTCCGGCTGCCACCTGATC
CTAACTTGCCTTGCATCCTGGTGGGCCCAGGGACTGGTATTGCACCCTTCCGGGGATTCTGGCAAGACCG
ATTACACGACATTGAGATCAAAGGACTGCAGCCTGCCCCCATGACTTTGGTGTTTGGCTGCCGATGCTCC
CAACTGGACCATCTCTACCGGGACGAGGTACTGGACGCCCAGCAGCGTGGAGTGTTTGGACAAGTCCTCA
CCGCCTTTTCCAGGGATCCTGGCAGCCCTAAGACCTATGTGCAAGACCTCCTGAGGACAGAGCTGGCCGC
GGAGGTTCACCGCGTGCTGTGCCTCGAGCAAGGACACATGTTTGTCTGCGGTGATGTCACTATGGCAACC
AGCGTCCTGCAAACCGTGCAGCGAATTCTGGCAACAGAGGGCAGCATGGAGCTGGATGAAGCCGGTGACG
TCATCGGCGTGCTGCGGGATCAGCAACGCTACCACGAGGACATTTTCGGACTCACATTGCGCACCCAGGA
GGTGACGAGCCGCATCCGCACCCAGAGTTTTTCTTTGCAGGAGCGACAGCTGAGGGGTGCAGTGCCCTGG
TCCTTTGACCCGCCTACCCAAGAAACACCTGGTTCCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_021838
ORF Size 3609 bp
Insert Size 3609
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Reference Data
RefSeq NM_021838.2, NP_068610.1
RefSeq Size 3953
RefSeq ORF 3609
Locus ID 24600
Gene Summary enzyme that synthesizes Nitric oxide from L-arginine [RGD, Feb 2006]

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