Ptbp2 (NM_001005555) Rat Untagged Clone

CAT#: RN201033

Ptbp2 (untagged ORF) - Rat polypyrimidine tract binding protein 2 (Ptbp2), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Ptbp2
• Synonyms: Ptb2; PTBLP; PTBLP-L; PTBLP-S
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN201033 representing NM_001005555
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGGAATTGTCACTGAGGTTGCTGTTGGTGTGAAGAGAGGATCTGACGAGCTACTCTCAGGCAGTG
  TTCTCAGTAGTCCCAACTCTAATATGAGTGGCATGGTAGTTACAGCCAATGGTAACGACAGTAAGAAATT
  TAAAGGAGAAGATAAGATGGACGGGGCGCCCTCTCGTGTGCTTCACATTCGAAAGTTACCTGGTGAAGTG
  ACTGAGACCGAAGTAATTGCGTTAGGCTTACCTTTTGGTAAGGTGACTAACATCCTTATGCTGAAAGGAA
  AGAACCAGGCATTTTTGGAACTGGCAACAGAGGAAGCAGCTATTACTATGGTTAATTACTATTCTGCTGT
  GACACCTCATCTTCGTAACCAACCAATTTACATTCAGTACTCCAATCACAAAGAACTAAAGACAGATAAC
  ACATTAAACCAACGTGCTCAAGTAGTTCTTCAAGCTGTGACAGCTGTCCAGACAGCAAATACACCTCTCA
  GTGGCACCACAGTCAGTGAGAGTGCGGTGACTCCAGCTCAGAGTCCAGTGCTTAGAATAATTATTGACAA
  TATGTACTACCCTGTAACACTTGATGTCCTTCACCAAATATTTTCTAAGTTTGGTGCTGTATTGAAGATA
  ATCACATTTACAAAAAACAACCAGTTTCAGGCTTTGCTCCAGTATGGTGATCCAGTAAACGCTCAACAGG
  CAAAGCTAGCCCTAGATGGTCAAAATATTTATAATGCTTGCTGTACCCTAAGGATTGATTTTTCCAAACT
  TGTGAATTTGAATGTAAAATACAACAATGATAAAAGTAGGGATTATACACGACCTGATCTTCCATCTGGA
  GACGGCCAGCCTGCATTAGACCCAGCTATTGCTGCAGCATTTGCCAAGGAGACGTCCCTTTTAGCTGTCC
  CAGGAGCTCTCAGTCCTTTGGCTATTCCTAATGCTGCTGCGGCAGCTGCTGCCGCTGCCGCTGGCCGAGT
  GGGCATGCCTGGAGTCTCAGCTGGTGGCAACACAGTCCTGTTGGTTAGCAATTTAAATGAAGAGATGGTT
  ACGCCCCAAAGTCTGTTTACCCTCTTCGGTGTTTATGGCGATGTGCAGCGCGTGAAGATTTTGTACAATA
  AGAAAGACAGTGCTCTGATACAAATGGCCGATGGGAACCAGTCACAGCTTGCCATGAATCATCTTAATGG
  GCAGAAGATGTATGGAAAAATTATTCGTGTTACTCTCTCTAAACATCAGACTGTGCAACTACCTCGAGAG
  GGACTTGATGATCAAGGGCTAACAAAAGATTTTGGGAATTCACCCCTGCACCGTTTTAAAAAACCGGGAT
  CCAAAAACTTTCAGAACATTTTCCCTCCTTCTGCAACCCTTCACCTGTCCAACATCCCTCCTTCTGTAGC
  AGAAGAGGATCTGCGAACTCTGTTTGCCAACACCGGGGGCACTGTGAAAGCATTTAAGTTTTTTCAAGAT
  CACAAAATGGCTCTTCTTCAGATGGCAACAGTGGAGGAAGCTATTCAGGCTTTGATTGATCTTCATAATT
  ATAACCTTGGTGAAAACCATCATCTGAGAGTGTCTTTCTCCAAATCAACAATTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001005555
• Insert Size: 1596 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005555.1, NP_001005555.1
• RefSeq Size: 1842 bp
• RefSeq ORF: 1596 bp
• Locus ID: 310820
• Cytogenetics: 2q41
• Gene Summary: RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. In addition to its function in pre-mRNA splicing, plays also a role in the regulation of translation. [UniProtKB/Swiss-Prot Function]