Polr3k (NM_001014259) Rat Untagged Clone

CAT#: RN201422

Polr3k (untagged ORF) - Rat polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa (Polr3k), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Polr3k
• Synonyms: MGC109430
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN201422 representing NM_001014259
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTGTTCTGCCCGGGCTGCGGGAACGGCCTCATTGTGGAGGAGGGGCAGCGCTGTCACCGCTTCG
  CCTGCAACACGTGCCCGTACGTCCATAACATCACCCGCAAGGTAACAAACCGAAAGTATCCAAAGCTAAA
  AGAAGTGGATGATGTGCTTGGTGGAGCAGCTGCCTGGGAGAATGTGGACTCTACTGCAGAGCCATGTCCA
  AAATGTGAGCATCCTCGTGCCTACTTCATGCAGCTTCAGACCCGTTCTGCGGACGAGCCAATGACCACCT
  TCTATAAGTGCTGCAATGCTCAGTGTGGACATCGCTGGAGGGATTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001014259
• Insert Size: 327 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014259.1, NP_001014281.1
• RefSeq Size: 995 bp
• RefSeq ORF: 327 bp
• Locus ID: 366277
• Cytogenetics: 3q43
• Gene Summary: estrogen responsive protein; may be regulated by 17beta-estradiol [RGD, Feb 2006]