Wee1 (NM_001012742) Rat Untagged Clone

CAT#: RN202092

Wee1 (untagged ORF) - Rat wee 1 homolog (S. pombe) (Wee1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Wee1
• Synonyms: MGC105683
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN202092 representing NM_001012742
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTTCCTGAGCCGACAGCAGCCGCCGCCCACCCGCCGCGCCGCAGCCGCCTGCAGTTTGCGGCAGA
  AGCTCATCTTCTCACCCGGCAGCGATTGTGAGGAGGAAGAGGAGGAGGAGGAGGAAGGCAGCGGCCACAG
  TACGGGGGAGGACTCGGCCTTCCAGGAGCCTGATTCGCCGCTGCCCTCTGCACGCAGCCCTGCCGAGGCC
  GAGGCCGAGCGCCGCCGCCGCTCGCCCGGCGCGGAGCCCAGCAGCCCGGGAGAGCTGGAGGAGGACTTGC
  TGCTGCGGGGCGGCGGCGGGGGCGCGCAAGCGGCGGGCGGAGGAGCCGAGGGCGACTCTTGGGAGGAGGA
  GGGTTTCGGCTCTTCGTCGCCCGTCAAGTCACCTACGACTGCCTACTTTCTGGGCAGCTCGTTCTCGCCG
  GTGCGCTGCGGCGGCCCTGGGGATGCCTCCCCGCGGGGCTGCGGGGTCCCGCGAGCCATGGACGACCCCT
  GTTCTCCGCAGCCCGACTACCCGAGCACCCCTCCGCACAAGACCTTCCGCAAGTTGCGCCTGTTCGACAC
  GCCGCACACTCCCAAGAGTTTGCTTTCCAAAGCTCGAGTTATTGATTCCAGCTCTGTTAAACTCCGGGGT
  AGTTCTCTATTCATGGACACTGAAAAATCAGGAAAAAGAGAATTCGACACACGGCAAACTCCTCAAGTGA
  ATATTAACCCCTTTACTCCGGATCCTGTAATGCTTCATTCCTCAGGACAGTGTCGTGGGAGAAAGAGAGC
  ATATTTTAATGATTCCTCTGAAGACATGGAAGCCAGTGATTATGAGTTTGAAGATGAAACAAGACCTGCC
  AAAAGAATTACAATTACTGAAAGCAATATGAAGTCACGATATACAACTGAATTTCATGAGCTGGAGAAAA
  TTGGTTCTGGAGAATTTGGTTCTGTGTTTAAATGTGTGAAGAGGCTAGATGGATGCATTTATGCCATTAA
  ACGATCAAAAAAGCCATTGGCTGGCTCTGTTGATGAACAGAATGCTTTGAGAGAAGTGTATGCTCATGCT
  GTGCTTGGACAGCATCCCCATGTGGTTCGTTATTTCTCTGCCTGGGCAGAAGATGACCATATGCTTATAC
  AGAATGAATACTGTAATGGTGGGAGTTTAGCTGACGCTGTAAGTGAGAACTACAGAGTCATGAGCTACTT
  TACTGAAGCAGAGCTGAAGGATCTCCTTTTGCAAGTTGGCAGGGGCTTGAGATACATACATTCAATGTCT
  TTGGTTCACATGGATATAAAACCTAGTAATATTTTTATATCTCGAACCTCAATCCCAAATGCAGTCTCTG
  AAGAAGGAGATGAAGATGACTGGATATCCAACAAAGTTATGTTTAAAATAGGTGATCTTGGGCATGTAAC
  AAGAATCTCTAGTCCACAAGTTGAAGAAGGTGATAGTCGTTTTCTAGCAAATGAAGTTTTACAAGAGAAC
  TATAGCCATCTACCGAAAGCAGATATTTTTGCTCTTGCTCTCACAGTCGTATGTGCTGCTGGTGCTGAAC
  CCCTTCCCAGAAATGGAGACCAGTGGCATGAAATCCGACAGGGTCGATTACCTCGGATTCCACAAGTGCT
  TTCCCAGGAGTTGACAGAGTTGCTAAAAGTTATGATTCATCCTGATCCAGAGAGAAGACCTTCAGCAATG
  GTGCTGGTGAAGCATTCAGTGTTGCTGTCTGCCTCTAGAAAGAGCGCAGAGCAGTTACGAATAGAATTGA
  ATGCTGAAAAATTCAAAAATTCTCTTTTGCAGAAAGAACTCAAGAAAGCCCAAATGGCAGCCAAAGTTGC
  AGCTGAGGAACGGGCACTCCTCACAGATCGGATGGCCACTAGATCTACCACCCAGAGTAATAGAACTTCT
  CGACTTATTGGAAAGAAAATGAACCGCTCTGTCAGCCTTACTATATACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001012742
• Insert Size: 1941 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012742.1, NP_001012760.1
• RefSeq Size: 3305 bp
• RefSeq ORF: 1941 bp
• Locus ID: 308937
• Cytogenetics: 1q33