Ldhb (NM_012595) Rat Untagged Clone

CAT#: RN202177

Ldhb (untagged ORF) - Rat lactate dehydrogenase B (Ldhb), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Ldhb
• Synonyms: Ldh2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN202177 representing NM_012595
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAACCCTTAAGGAAAAGCTCATTGCGCCAGTCGCAGACGACGAGACTGCCGTCCCGAACAACAAGA
  TTACTGTAGTAGGCGTTGGACAAGTTGGAATGGCTTGTGCTATCAGCATTCTGGGGAAGTCTCTGGCTGA
  TGAGCTTGCCCTGGTGGATGTCTTGGAAGACAAGCTCAAAGGAGAAATGATGGATCTGCAGCACGGGAGC
  TTATTTCTCCAGACTCCGAAAATCGTGGCTGATAAAGATTACTCCGTGACAGCCAATTCTAAGATTGTGG
  TGGTGACCGCGGGAGTCCGCCAGCAGGAGGGGGAGAGTCGGCTCAACCTGGTGCAGAGAAACGTCAATGT
  ATTCAAGTTCATTATTCCTCAGATCGTCAAGTACAGCCCCGACTGCACCATCATCGTGGTTTCCAACCCA
  GTGGATATTCTTACCTATGTCACCTGGAAGCTGAGCGGGCTACCTAAGCACCGCGTGATTGGAAGTGGAT
  GCAATCTGGATTCTGCTCGGTTTCGTTACCTCATGGCCGAAAAGCTTGGTATTCATCCCAGCAGCTGCCA
  CGGCTGGATCCTGGGCGAGCACGGGGACTCCAGTGTGGCAGTGTGGAGCGGGGTGAATGTGGCAGGAGTC
  TCCCTCCAGGAACTGAACCCAGAGATGGGAACGGACAATGACAGCGAGAACTGGAAGGAGGTGCATAAGA
  TGGTGGTGGACAGTGCCTATGAAGTCATCAAGCTAAAAGGCTACACCAACTGGGCCATCGGCCTAAGTGT
  GGCTGACCTCATCGAATCCATGCTGAAAAACCTCTCTCGGATTCACCCCGTGTCTACAATGGTGAAGGGA
  ATGTACGGCATCGAGAACGAAGTCTTCCTCAGTCTCCCGTGCATCCTTAATGCTCGGGGACTGACCAGCG
  TCATCAACCAGAAGCTGAAGGACGATGAGGTCGCTCAGCTCAGGAAGAGTGCGGACACCCTGTGGGATAT
  CCAGAAAGACCTCAAGGACCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_012595
• ORF Size: 1005 bp
• Insert Size: 1005
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_012595.2, NP_036727.1
• RefSeq Size: 1345
• RefSeq ORF: 1005
• Locus ID: 24534
• Gene Summary: This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have also been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 9. [provided by RefSeq, Feb 2016]
  Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (Ldhb) results from translation termination at the upstream UGA stop codon, while the longer isoform (Ldhbx) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (Ldhb), which is localized in the cytosol. Variants 1 and 2 encode the same isoform.