Ldhb (NM_012595) Rat Tagged ORF Clone

CAT#: RR202177

Ldhb (Myc-DDK-tagged ORF) - Rat lactate dehydrogenase B (Ldhb), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ldhb
• Synonyms: Ldh2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202177 representing NM_012595
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAACCCTTAAGGAAAAGCTCATTGCGCCAGTCGCAGACGACGAGACTGCCGTCCCGAACAACAAGA
  TTACTGTAGTAGGCGTTGGACAAGTTGGAATGGCTTGTGCTATCAGCATTCTGGGGAAGTCTCTGGCTGA
  TGAGCTTGCCCTGGTGGATGTCTTGGAAGACAAGCTCAAAGGAGAAATGATGGATCTGCAGCACGGGAGC
  TTATTTCTCCAGACTCCGAAAATCGTGGCTGATAAAGATTACTCCGTGACAGCCAATTCTAAGATTGTGG
  TGGTGACCGCGGGAGTCCGCCAGCAGGAGGGGGAGAGTCGGCTCAACCTGGTGCAGAGAAACGTCAATGT
  ATTCAAGTTCATTATTCCTCAGATCGTCAAGTACAGCCCCGACTGCACCATCATCGTGGTTTCCAACCCA
  GTGGATATTCTTACCTATGTCACCTGGAAGCTGAGCGGGCTACCTAAGCACCGCGTGATTGGAAGTGGAT
  GCAATCTGGATTCTGCTCGGTTTCGTTACCTCATGGCCGAAAAGCTTGGTATTCATCCCAGCAGCTGCCA
  CGGCTGGATCCTGGGCGAGCACGGGGACTCCAGTGTGGCAGTGTGGAGCGGGGTGAATGTGGCAGGAGTC
  TCCCTCCAGGAACTGAACCCAGAGATGGGAACGGACAATGACAGCGAGAACTGGAAGGAGGTGCATAAGA
  TGGTGGTGGACAGTGCCTATGAAGTCATCAAGCTAAAAGGCTACACCAACTGGGCCATCGGCCTAAGTGT
  GGCTGACCTCATCGAATCCATGCTGAAAAACCTCTCTCGGATTCACCCCGTGTCTACAATGGTGAAGGGA
  ATGTACGGCATCGAGAACGAAGTCTTCCTCAGTCTCCCGTGCATCCTTAATGCTCGGGGACTGACCAGCG
  TCATCAACCAGAAGCTGAAGGACGATGAGGTCGCTCAGCTCAGGAAGAGTGCGGACACCCTGTGGGATAT
  CCAGAAAGACCTCAAGGACCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202177 representing NM_012595
  Red=Cloning site Green=Tags(s)
  
  MATLKEKLIAPVADDETAVPNNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGS
  LFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKFIIPQIVKYSPDCTIIVVSNP
  VDILTYVTWKLSGLPKHRVIGSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGV
  SLQELNPEMGTDNDSENWKEVHKMVVDSAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVSTMVKG
  MYGIENEVFLSLPCILNARGLTSVINQKLKDDEVAQLRKSADTLWDIQKDLKDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012595
• ORF Size: 1002 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012595.1, NM_012595.2, NP_036727.1
• RefSeq Size: 1217
• RefSeq ORF: 1005
• Locus ID: 24534
• MW: 36.6 kDa
• Gene Summary: This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have also been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 9. [provided by RefSeq, Feb 2016]