Smpd1 (NM_001006997) Rat Untagged Clone

CAT#: RN202346

Smpd1 (untagged ORF) - Rat sphingomyelin phosphodiesterase 1, acid lysosomal (Smpd1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Smpd1
• Synonyms: MGC94748
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN202346 representing NM_001006997
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCACCACGGAGCGTCATCCGGCCAGGGCCATCTCAGAGCCGGCTGGGAGCAGAGACTGGAGAGGT
  CCTTACCGGCACCCAAATTGGGACTCCTTTGGATGGGGCTGGGCTTGGCTCTGGTCCTGGCTCTGTTTGA
  CTCCACGGTTCTCTGGGTTCCTGCCAGAGCTTATCCTCTTCCCTCCCAAGGCCATTCTGTCAAATTCAGT
  GCGGTAGCGCCGCAGCTCCAGAATGCCTTTGGGTGGAGGAACCTCACCTGCCCCGCCTGCAAAGTCTTAT
  TTACTGCTCTCAACTATGGGCTGAAGAAGGAGCCCAATGTGGCACGAGTAGGCTCTGTGGCCATCAAGAT
  GTGCAAGATGCTGAACATAGCGCCACTAAATGTGTGCCAGTCAGCTGTTCATCTCTTTGAGGACGATGTG
  GTGGACGTGTGGACACGCTCAGTTCTGAGCCCTTCAGAAGCTTGTGGCTTGCTTCTGGGCTCCTCTTGTG
  GGCACTGGGACATCTTTTCAACTTGGAACATCTCTTTGCCATCAGTGCCGAAGCCACCCCCAAAGCCACC
  GAGCCCACCAGCCCCAGGTGCCCCTGTCAGCCGTGTCCTCTTCCTTACTGACCTTCACTGGGACCATGAC
  TACCTGGAGGGCACAGACCCTAACTGTGCAGATCCCCTCTGCTGCCGCAGGGGGTCTGGATGGCCGCCCA
  ACTCACGGACAGGGGCTGGGTACTGGGGAGAGTACAGCAAGTGTGACCTGCCCCTGCGAACGCTAGAGAG
  CCTGTTGAAAGGGCTGGGCCCCGCCGGCCCTTTTGAAATGGTGTACTGGACAGGAGACATCCCTGCCCAT
  GATGTCTGGCAACAGTCTCGACAAGATCAGCTAAGGGCCCTGAACACCATCACAGACCTCGTGTGGAAGT
  TCTTGGGCCCTGTACCGGTGTACCCTGCTGTGGGCAACCATGAGAGTACTCCTGTCAATGGCTTCCCTCC
  CCCCTTCATAAAGGGAAACCAGTCTTCACAATGGCTTTATGAAGCCATGGCCAAGGCATGGGAACCCTGG
  TTACCAGCTGACGCCCTTCACACCCTCAGAATTGGGGGCTTCTATGCCCTCACCCCACGCCCTGGCCTCC
  GCCTCATCTCTCTCAATATGAATTTTTGTTCCCGTGAGAACTTTTGGCTCTTGATCAACTCCACAGATCC
  TGCTGGACAACTCCAGTGGCTGGTGGACGAGCTTCAGGCTGCTGAGAATCGAGGAGACAAAGTGCATATA
  ATTGGGCACATTCCTCCAGGACATTGCCTCAAGAGCTGGAGTTGGAACTATTACAAAATTATAGCCAGGT
  ATGAAAACACTCTGGCTGGTCAGTTCTTTGGCCACACTCATGTGGATGAGTTTGAGATCTTCTATGATGA
  GGAAACTCTGAGCCGCCCACTAGCCGTAGCCTTCCTGGGACCCAGTGCTACCACCTTTATCAACCTTAAT
  CCTGGCTACCGAGTTTACCAAATAGATGGAAACTACCCGGGAAGCTCTCATGTGGTCCTGGACCATGAGA
  CCTACATCCTGAATCTGACCCAGGCCAATGCGCCGGGGGGCACGCCCAGCTGGAAGCGGCTCTACAGGGC
  TCGGGAAACCTATGGACTTCCAGATGCGATGCCTGCCTCCTGGCACAACCTGGTCTACCGCATGAGGGCT
  GATGAGCAGCTCTTCCAGACCTTCTGGTTTCTCTACCATAAGGGCCACCCACCTTCAGAGCCCTGCGGCA
  CACCCTGCCGCCTGGCCACTCTGTGTGCCCAGCTCTCAGCACGTGCAGACAGCCCTGCTCTGTGTCGCCA
  CTTGATGCCCAATGGGAGCCTCCCAGATGCCCATAGCTTGTGGTCACGGCCCCTGCTGTGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001006997
• Insert Size: 1884 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001006997.1, NP_001006998.1
• RefSeq Size: 2334 bp
• RefSeq ORF: 1884 bp
• Locus ID: 308909
• Cytogenetics: 1q32