Btk (NM_001007798) Rat Untagged Clone

CAT#: RN202955

Btk (untagged ORF) - Rat Bruton agammaglobulinemia tyrosine kinase (Btk), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Btk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN202955 representing NM_001007798
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTGTGATACTGGAGAGCATCTTTCTGAAGCGCTCCCAGCAGAAAAAGAAAACATCACCTTTAA
  ATTTTAAGAAGCGCCTATTTCTCCTGACTGTGCACAAACTTTCATACTATGAATATGACTTTGAACGTGG
  GAGAAGAGGCAGTAAGAAAGGTTCAATAGATGTCGAGAAGATCACCTGTGTTGAAACAGTAGTTCCTGAA
  AAAAATCCCCCACCAGAAAGACAGATTCCAGTTCCGAGGGGTGAGGAGTCCAGTGAAATGGAACAGATTT
  CAATCATTGAAAGGTTCCCGTACCCATTTCAGGTCGTATATGATGAAGGACCTCTCTATGTTTTCTCCCC
  AACCGAAGAGCTGAGAAAACGCTGGATTCACCAGCTCAAAAATGTAATCCGGTACAATAGTGACCTGGTG
  CAGAAATATCATCCTTGCTTCTGGATTGATGGACAGTATCTCTGCTGCTCTCAGACAGCCAAGAATGCTA
  TGGGCTGCCAAATTTTGGAGAACAGGAATGGAAGCTTAAAACCTGGAAGTTCTCATCGAAAAACAAAAAA
  GCCTCTTCCCCCTACCCCAGAGGAAGATCAGATCTTGAAAAAGCCACTCCCCCCTGAGCCAACAGCAGCA
  CCAATCTCCACAAGTGAGCTGAAAAAGGTCGTGGCCCTTTATGATTATATGCCAATGAATGCAAATGACT
  TACAATTGCGAAAGGGCGAGGAGTATTTTATCCTGGAGGAGAGCAACCTACCATGGTGGCGAGCACGAGA
  TAAAAATGGGCAGGAAGGCTATATCCCCAGTAACTATGTCACCGAAGCAGAGGACTCCATAGAGATGTAT
  GAGTGGTATTCCAAGCACATGACTCGAAGTCAAGCTGAGCAACTTCTAAAGCAAGAGGGGAAAGAAGGAG
  GTTTCATTGTCAGAGACTCCAGCAAAGCTGGAAAATACACTGTGTCTGTGTTTGCTAAATCTACAGGGGA
  GCCTCAAGGGGTGATACGCCATTATGTTGTGTGCTCCACACCTCAGAGCCAGTATTACCTGGCTGAGAAG
  CACCTTTTCAGCACCATTCCTGAGCTCATCAACTACCATCAACATAACTCTGCAGGCCTCATATCCAGGC
  TGAAATATCCTGTATCGAAACAAAACAAAAACGCGCCTTCTACGGCAGGCCTGGGCTATGGATCATGGGA
  AATTGATCCAAAGGACTTGACCTTCTTGAAGGAGCTTGGGACTGGACAATTTGGTGTCGTCAAATATGGG
  AAGTGGAGGGGCCAATATGATGTGGCCATCAAGATGATCAGAGAAGGTTCCATGTCTGAGGATGAATTCA
  TTGAAGAAGCCAAAGTCATGATGAATCTTTCCCATGAGAAGCTAGTGCAGTTGTATGGAGTCTGCACCAA
  ACAGCGCCCCATCTTCATCATCACTGAATACATGGCTAATGGCTGCCTCTTGAACTACCTGAGGGAGATG
  CGGCACCGCTTCCAGACACAGCAGCTGCTTGAGATGTGCAAAGATGTCTGTGAAGCAATGGAATACTTGG
  AGTCAAAGCAGTTCCTTCATAGAGACCTGGCAGCTCGAAACTGTCTGGTAAATGATCAAGGAGTTGTGAA
  AGTATCTGACTTTGGCCTGTCTAGGTATGTTCTTGATGATGAATACACCAGCTCGGTAGGCTCCAAATTT
  CCAGTCCGGTGGTCTCCACCAGAAGTGCTCATGTATAGCAAATTCAGCAGCAAATCTGACATCTGGGCTT
  TTGGGGTTCTAATGTGGGAAATTTACTCCCTGGGGAAAATACCATATGAGAGATTTACTAACAGTGAGAC
  AGCAGAACACATTGCTCAAGGCTTACGTCTCTACAGGCCTCATTTGGCATCAGACAGGGTATATACCATC
  ATGTACAGCTGCTGGCATGAGAAAGCAGATGAACGTCCTAGTTTCAAAATTCTCTTGAGTAACATTCTAG
  ATGTCATGGATGAAGAATCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001007798
• Insert Size: 1983 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007798.1, NP_001007799.1
• RefSeq Size: 1983 bp
• RefSeq ORF: 1983 bp
• Locus ID: 367901
• Cytogenetics: Xq32