Wnk1 (NM_001002823) Rat Untagged Clone

CAT#: RN204274

Wnk1 (untagged ORF) - Rat hereditary sensory neuropathy, type II (Hsn2), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Wnk1
• Synonyms: Hsn2; Prkwnk1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN204274 representing NM_001002823
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGACGGCACCGCAGAGAAGCAGAGCGGCACTCCTGGCTTCCTTTCGCCTCCGGCTCCAGTCCCCA
  AGAATGGCTCAAGCTCCGATTCTTCCGTGGGCGAGAAACTGGGGGCCGCGGTCGCCGACTCCGGAATTGG
  CAGGACAGAGGAATACCGGCGCCGCCGGCACACTATGGACAAGGACAGTCGTGGGGCGGCCGCCACCACT
  ACCCCCACGGAGCACCGATTTTTCCGCCGAAGCGTCATCTGCGACTCCAATGCCACGGCGTTGGAGCTGC
  CGGGCCTCCCTCTCTCTATCCCTCAACCTAGTGTACCCGCAGTGGTGCCTCAGAGTGCTCCACCCGAGCC
  TCACAGAGAGGAAACTTTGACGGCCACCGTTGCTTCCCAGGTGTCTCAGCAACCCTCGGCTGCTGCCTCC
  CCTGGGGAACAAGCCGTTGTAGGCTCGGCCACCGCGACCGTCCCCAGCAGTACCAGCAAAGACCGCCCGG
  TGTCCCAGCCCAGCCTCGTGGGTAGCAAAGAGGAGCCGCCGCCGTCCAGAAGTGGAAGTGGCAGCGGTGG
  TGCCAGTGCCAAAGAGCCGCAGGAGGAAAGGAACCAGCAACAGGATGATATCGAAGAGCTGGAGACCAAG
  GCAGTGGGAATGTCCAATGACGGTCGCTTTCTCAAATTTGACATCGAAATCGGCAGAGGCTCCTTTAAGA
  CGGTCTACAAAGGTCTGGACACCGAAACCACTGTGGAAGTCGCCTGGTGTGAATTGCAGGATCGAAAGTT
  AACAAAATCTGAAAGGCAGAGATTCAAAGAAGAAGCTGAAATGCTAAAGGGTCTTCAACATCCTAACATT
  GTTCGATTCTATGATTCCTGGGAATCCACAGTAAAAGGGAAAAAATGCATTGTTTTAGTGACTGAACTAA
  TGACATCTGGAACACTTAAAACGTACTTAAAAAGGTTTAAAGTGATGAAAATCAAAGTTTTAAGAAGCTG
  GTGTCGGCAAATCCTTAAAGGACTTCAATTTCTCCATACACGAACTCCCCCGATTATTCACCGGGATCTT
  AAATGTGACAACATCTTTATCACTGGTCCTACCGGCTCAGTCAAGATCGGAGACCTTGGTCTGGCAACTC
  TAAAGCGGGCTTCTTTTGCCAAGAGTGTGATAGGTACCCCAGAGTTTATGGCTCCTGAGATGTATGAGGA
  GAAGTACGATGAATCCGTTGATGTTTATGCTTTTGGGATGTGCATGCTTGAGATGGCTACGTCTGAATAT
  CCATACTCAGAGTGCCAAAATGCTGCTCAGATCTACCGTCGAGTGACCAGTGGAGTGAAGCCAGCCAGTT
  TTGACAAAGTAGCAATTCCTGAAGTGAAGGAAATTATTGAAGGATGTATCCGACAAAACAAAGATGAAAG
  ATATTCCATCAAAGACCTTTTGAACCATGCCTTTTTCCAGGAGGAAACAGGGGTACGGGTAGAATTAGCA
  GAAGAAGATGATGGAGAAAAGATAGCTATCAAATTATGGCTACGTATTGAAGATATTAAGAAATTAAAGG
  GAAAATACAAAGACAATGAAGCTATTGAGTTTTCCTTTGACTTGGAGAGGGATGTACCAGAAGATGTTGC
  TCAAGAAATGGTCGAGTCTGGTTATGTCTGTGAAGGTGATCACAAGACCATGGCTAAAGCTATCAAAGAT
  AGGGTATCATTAATTAAGAGAAAACGAGAGCAGCGGCAGTTGGTTCGAGAAGAACAAGAAAAAAGAAAAC
  AAGAAGAGAGCAGTTTCAAACAGCAGAATGAACAACAAGCAAGTGTTTCCCAGGCAGGAATCCAGCCACT
  CTCTGTTGCTAGCACTGGCATACCCACTGCTCCCACCACATCCGCTTCAGTTTCTACTCAAGTAGAGCCT
  GAAGAACCCGAGGCAGATCAGCATCAACAGCTGCAGTATCAGCAACCTAGTATATCTGTGTTGTCTGATG
  GAACAGTTGACAGTGGTCAAGGATCATCTGTATTCACAGAATCCAGAGTGAGCAGCCAACAGACTGTTTC
  CTATGGCTCCCAACATGAGCAGGCACATTCTATTGGCACAGCACCAGGACATACAGTTTCTAGTATCCAA
  GCACAATCTCAGCCCCATGGGGTGTATCCACCCTCAAGTATGCCTCAGTCCGTGGCGCATCCGTGTGGGG
  GGACCCCAACATACCCAGAATCACAGATATTTTTCCCAACTATTCATGAACGTCCAGTTTCTTTTTCACC
  ACCTCCTACCTGTCCACCGAAAGTAGCCATTTCCCAGCGACGTAAGAGTACCTCCTTCCTGGAAGCTCAA
  ACTCGCCACTTCCAACCCCTGCTGAGGACTGTTGGCCAAAATCATCTTCCACCTGGTGGCAGCCCAACTA
  ACTGGACACCAGAGGCCATAGTTATGTTGGGTACTACAGCCAATAGAGTAAATAGAGAGCTATGTGAGAT
  GCAGGTCCAACCTGTGTTTGAGACAACCCAGATTTACAGTGACTATAGACCTGGACTAGTACTGGCAGAA
  GAAGCTCACTATTTTATTCCTCAGGAAACAGTATATCTAGCTGGGGTGCATTACCAGGCCCATGCGGCAG
  GACAGTATGAGGGTATTTCATACAACTCACCAGTACTGTCAAGTCCTATGAAACAGATAACTGAACAGAA
  GCCAGTGCCTGGGTGCCCTGCCTCAAGTTCTGTCTTTGAATTTCCTTCTGGACAGGCTTTCCTAGTAGGA
  CACCTTCAGAATTTAAGATTAGATTCTGGACCAAGTCCAGCATCACCCCTGTCTAGTATTTCTGCGCCTA
  ACAGTACAGATGCTACACATTTGAAATTTCACCCTGTCTTTGTACCTCATTCTGCACCAGCTGTGTTAAC
  TCATAGCAATGAGAACAGAAGCAACTGTGTATTTGAATTTCATGCTCAAACACCAAGTTCCTCTTCAGGA
  GAAGGAGGTGGGATTTTACCTCAACGTGTTTACCGAAATCGACAGGTTGCAGTGGACTCAAGTCAGGAAG
  AACTGTCTCCTCAGTCAGTTGGATTACACTGCCACCTGCAGCCTGTTACTGAAGAACAGCGTAATAACCA
  TACCCCAGAATTGACCATTTCTGTGGTAGAACCTATGGGACAAAACTGGCCAGTAGGAAGCCCAGAATAT
  TCCAGTGATTCCTCTCAAATTACTTCTTCAGATATCAGTGATTTTCAATCACCTCCCCCTACAGGGGGAA
  CAGCTGCACCTTTTGGCTCTGACGTCTCATTGCCCTATATTCGCCTGCCTCAGACAGTGTTACAAGAATC
  CCCACTATTCTTCTGTTTCCCCCAAGGAACCACATCTCAGCAGGTCTTATCTGCTTCATATTCTTCAGGA
  GGATCTGCACTCCATCCACAGGCACAGGGGCAGAACCAGGGCCAGCCATCAAGTAGTTTAGCAGGGGTTC
  TATCTTCACAACCCGTCCAACATCCTCAGCAGCAGGGAATACAGCCGACTGTACCTCCTCAACAGGCAGT
  ACAGTATTCACTTCCACAGGCAGCGTCTTCCAGTGAAGGCACTGTCCAGCCAGTGAGTCAGCCTCAAGTG
  TCAGCTGGAACACAGGGCTTCCCATCTCGACTGCCACCACAATACCCAGGAGATTCAAATATTGCTCCCT
  CTTCCAACGTGGCTTCTGTTTGCATCCATTCTACAGTCCTAGCCCCTCCTCCTATGCCAACAGAAGCATT
  GGCCACCCAAGGTTACTTTCCTACAGTAGTGCAGCCTTATGTGGAATCAACTCCTTTGGTTCCTATGGGC
  AGTGTAGGAGGCCAGGTTCAAGTGTCCCAACCAGCAGTGAGTTTATCGCAACAACCCCCCACTACATCCT
  CCCAGCAAGCAGTTTTGGAGAGTAGTACACAGGGAGTCTCTCAGGCTGCTCCTCCAGAACAAACTCCAAT
  AACACAGTCACAACCTACTCAACCTGTTCCATTGGTTTCCTCTGTGGACAGTGCCCATTCAGATGTTGCT
  TCTGGTATGAGTGATGGCAATGAGAATGCTCCTTCATCCAGCGGGAGGCATGAAGGGAGGACGACAAAAC
  GTCATTATCGAAAATCTGTAAGAAGTCGTTCTCGTCATGAAAAGACTTCTCGCCCGAAACTGAGGATTTT
  AAATGTTTCAAATAAAGGAGACCGAGTAGTAGAATGTCAATTGGAGACTCATAATCGGAAAATGGTTACA
  TTTAAATTTGACCTAGATGGTGACAACCCTGAAGAGATAGCAACAATTATGGTGAACAATGACTTTATTC
  TAGCCATAGAGAGAGAGTCTTTTGTGGCTCAAGTGCGGGAAATTATTGAAAAGGCTGATGAAATGTTAAG
  CGAGGATGTCAGTGTGGAGCCTGAGGGTGACCAGGGATTGGAGAGTCTACAAGGAAAGGATGACTATGGC
  TTTCCAGGTTCTCAGAAATTAGAAGGAGAGTTCAAGCAACCAATTGCTGTATCTTCCATGCCACAACAAA
  TAGGTGTTCCTACCAGTTCTTTAACACAAGTTGTTCATTCTGCTGGAAGACGATTTATAGTGAGTCCAGT
  TCCAGAAAGTCGATTACGAGAATCAAAAATTTTCACCAGTGAAATACCAGATCCAGTTGCGGCCTCTACA
  TCTCAGGGCCCTGGAATGAACTTATCTCACTCCGCTTCATCCCTTAGTCTCCAGCAGGCTTTTTCTGAAC
  TGAAACATGGTCAGATGACTGAAGGACCCAATACAGCACCTCCAAATTTTAATCACCCAGGACCAACATT
  TTCTCCCTTTTTGACTAGCATTGCTGGTGTCCAAACTGTAGCAGCATCCACACCATCAGTATCAGTCCCT
  ATAACAAGCAGCCCTCTTAATGACATTTCCACATCAGTGATGCAGTCAGAGGGTGCACTGCCCACTGACA
  AAGGCATTGGGGGAGTTACCACCAGCACAGGTGTGGTAGCTTCAGGTGGTCTCACCACATTGTCTGTGTC
  TGAGACACCAACACTTTCCAGTGCAGTTTCAAGTTCTACAGCGCCTGCAGTTGTCACAGTGTCTACAACA
  TCCCAACCAGTTCAGGCTTCCACATCTGGGAGCATTGCTTCTAGTACAGGCAGTTTTCCTTCTGGAACAT
  TTTCCACAACTACAGGTACCACAGTGAGTAGTGTAGCTGTCCCAAATGCTAAGCCTCCAACTGTATTACT
  GCAACAGGTAGCAGGTAATACTGCTGGGGTAGCCATAGTGACATCAGTTTCTACTACCACTCCATTCCCA
  GCCATGGCTTCACAGCCATCCCTTCCACTTGGTAGCAGCACATCTGCTCCTACACTAGCTGAAACAGTGG
  TGGTTAGTGCACACTCACTAGACAAGGCATCTCACAGTAGTACAGCAGGATTGGGTTTGTCCTTTTGCGC
  ACCGTCCTCTTCTTCCTCCTCTGGAACAGCAGTGTCTAGTTCTGTTTCTCAGCCTGGTATTGTGCATCCT
  TTGGTCATCTCATCAGCTATAGCTTCTACACCTGTCCTCCCACAACCAGCAGTACCTACTTCTACACCTT
  TATTACCCCAAGTACCTAATATCCCACCCTTGGTACAACCTGTTGCTAATGTGCCTGCTGTACAGCAAAC
  ACTCATTCATAGTCAGCCTCAACCAGCTCTACTTCCCAACCAGCCTCACACACACTGTCCCGAAATGGAT
  GCTGATACCCAGTCCAAAGCTCCTGGGATCGATGATATAAAGACTCTGGAGGAAAAGCTGAGATCGCTCT
  TTAGTGAGCATAGCTCATCTGGAACTCAGCATGCTTCTGTCTCCCTGGAGACACCCTTAGTAGTAGAGAC
  TGTCACACCGGGCATCCCTACCACTGCTGTAGCACCAAGTAAACTCATGACTTCCACTACAAGCACTTGC
  TTGCCACCAACCAATTTACCCTTAGGAACAGCTGGCATGCCAGTTATGCCAGTGGGCACACCTGGTCAAG
  TTTCTACCCCAGGCACACATGCCTCAGCCCCAGCCAGCACCGCAACAGGAGCAAAACCTGGAACAACTCC
  CCCAAAGCCATCTTTAACTAAGACTGTGGTGCCGCCAGTGGGTACTGAGCTTTCGGCTGGTACTGTACCC
  TGTGAGCAGCTGCCACCTTTTCCCGGACCGTCTCTAATTCAGACCCAGCAACCCCTAGAAGATCTTGATG
  CTCAGTTGAGAAGAACACTTAGTCCAGAGACTATTCCAGTGACACCCGCAGTTGGTCCTTTGTCTACGAT
  GTCTTCAACGGCAGTTACAGAAGCAGGAAGTCAACCTCAGAAGGATGGCACAGAAGTTCATGTCACAGCA
  AGTAGCTCAGGAGCTGGTGTTGTTAAGATGGGGAGATTTCAGGTTTCAGTTACAATGGATGATGCCCAGA
  AAGAGCGTAAAAATAGGTCAGAAGATACAAAGTCGGTTCATTTTGAATCTAGTACATCAGAGTCATCAGT
  TCTTTCAAGTAGTAGTCCAGAGAGTACCCTGGTAAAGCCAGAGCCTAATGGAATTACTGTTTCTGGCATC
  TCCTTAGATGTGCCAGACAGTACCCATAGAACACCGACCCCAGAAGCAAAGTCAGAAACAGGACAGCCTA
  CCAAGGTTGGACGTTTTCAGGTGACAACTACAGCCAACAAAGTGGGTCGTTTCTCTGTTTCAAGAACTGA
  GGACAAGGTCACAGAGCTCAAAAAAGAGGGACCAGTGACCTCTCCTTTCAGAGATTCAGAACAAACTGTT
  ATTCCTGCTGCGATACCAAAGAAGGAGAAGCCTGAGCTGGCAGAACCTTCCCATCTGAATGGGCCATCTT
  CAGACTTGGAGGCTGCCTTTCTAAGTAGGGGTGGAGAGGATGGATCAGGTAGTCCTCACTCTCCCCCTCA
  CCTGTGTTCAAAGAGCCTTCCCATCCAGACTCTAAGTCAAAGCCTTAGTAATTCATTCAACTCCTCTTAC
  ATGAGTAGTGACAATGAGTCCGACATTGAAGATGAAGACTTAAGGTTAGAACTGCGACGACTACGGGAAA
  AACATCTTAAAGAGATTCAGGACCTGCAGAGTCGCCAGAAGCATGAAATTGAATCTTTGTATACCAAACT
  GGGCAAGGTTCCCCCTGCTGTCATTATTCCCCCAGCTGCTCCTCTGTCAGGGAGAAGAAGGAGGCCTACT
  AAAAGCAAAGGCAGCAAGTCCAGTCGCAGCAGCTCATTGGGCAACAAAAGCCCTCAGCTTTCAGGTAACC
  TGTCTGGTCAGAGTGGAACTTCAGTCTTAAACCCCCAACAGACCCTCCATCCCCCAGGCAATACCCCAGA
  GACTGGACACAACCAGCTGTTACAGCCTCTTAAGCCATCTCCCTCCAGTGACAACCTCTATTCAGCCTTT
  ACCAGTGATGGTGCCATTTCAGTACCAAGCCTTTCTGCTCCAGGTCAAGGGACCAGCAGCACAAACACCG
  TTGGAGGAACAGTGAGTAGCCAGGCTGCCCAGGCTCAGCCTCCTGCCATGACTTCCAGTAGGAAGGGCAC
  ATTCACAGATGACCTGCACAAGTTGGTAGACAACTGGGCCCGAGATGCCATGAATCTTTCAGGCAGGAGA
  GGCAGCAAAGGACACATGAATTATGAGGGCCCTGGAATGGCAAGGAAGTTCTCTGCTCCTGGGCAGCTGT
  GCATTTCCATGACCTCAAACATGGGTGGCTCTACCCCCATCTCTGCAGCATCTGCTACCTCTCTAGGTCA
  CTTCACCAAGTCCATGTGCCCCCCACAGCAGTATGGTTTTCCAGCTGCCCCATTCGGCACTCAGTGGAGT
  GGAACAGGTGGTCCAGCACCACAGCCACTTGGTCAGTTCCAACCTGTAGGAACCACATCCTTGCAAAATT
  TCAACATCAGCAATTTGCAGAAGTCCATCAGCAACCCCCCAGGTTCCAACCTACGGACCACCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001002823
• Insert Size: 7905 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002823.2, NP_001002823.2
• RefSeq Size: 10497 bp
• RefSeq ORF: 7905 bp
• Locus ID: 116477
• Cytogenetics: 4q42
• Gene Summary: catalyzes the phosphorylation of myelin basic protein in vitro; has autophosphorylation activity; may regulate the thiazide-sensitive Na-Cl cotransporter NCC [RGD, Feb 2006]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). This variant represents the exon combination of the dorsal root ganglia and sciatic nerve variant inferred from the mouse sequence described in Figure 2F of PubMed ID 18521183. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.