Dagla (NM_001005886) Rat Untagged Clone

CAT#: RN204355

Dagla (untagged ORF) - Rat diacylglycerol lipase, alpha (Dagla), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Dagla
• Synonyms: Nsddr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN204355 representing NM_001005886
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGGGATCGTGGTGTTCCGGCGGCGCTGGTCTGTGGGCAGCGATGACCTCGTGCTGCCTGCTATCT
  TCCTCTTCCTGCTGCACACCACCTGGTTTGTGATCCTGTCTGTGGTGCTCTTCGGCCTGGTCTACAACCC
  GCACGAGGCCTGCTCCCTGAACCTGGTGGACCACGGCCGAGGCTACCTGGGCATCCTTCTGAGCTGTATG
  ATTGCTGAGATGGCTATCATTTGGCTGAGCATGCGTGGGGGTATCCTCTACACAGAACCCCGGGACTCCA
  TGCAGTACGTGCTCTACGTGCGCCTGGCCATTTTGGTGATCGAGTTCATCTACGCTATCGTGGGCATCGT
  CTGGCTTACACAGTACTACACCTCCTGCAATGACCTCACTGCTAAGAATGTCACTCTCGGGATGGTCGTC
  TGCAACTGGGTGGTCATCCTCAGCGTCTGCATCACTGTCCTCTGTGTCTTTGACCCCACTGGCCGCACCT
  TCGTCAAGCTGAGAGCCACCAAGAGGAGGCAGCGCAACCTGCGGACTTACAACCTGCGGCATCGGTTAGA
  GGAGGGTCAGGCCACCAGCTGGTCCCGCCGTCTAAAAGTGTTCCTCTGTTGCACCCGGACAAAGGATTCC
  CAGTCAGATGCCTATTCAGAAATTGCCTACCTCTTTGCTGAATTTTTCCGTGACCTCGACATAGTGCCCT
  CCGACATCATTGCTGGCCTGGTGCTGCTTAGGCAGCGGCAGCGAGCCAAGCGCAATGCGGTGCTGGATGA
  GGCAAACAATGACATCTTGGCTTTCCTGTCTGGGATGCCAGTGACCAGAAACACCAAGTACCTCGACCTC
  AAGAACTCGCACGAGATGCTACGCTACAAAGAAGTCTGCTACTACATGCTCTTTGCCCTCGCTGCCTATG
  GCTGGCCCATGTACCTGATGAGGAAGCCCACCTGTGGCCTCTGCCAGCTGGCTCGGTCCTGCTCGTGTTG
  TCTCTGCCCTGCACGCCCCCGATTCGCCCCTGGGGTCACCATTGAGGAAGACAACTGTTGTGGCTGCAAT
  GCAATTGCCATCCGCCGTCACTTCCTGGATGAGAACATGACTGCTGTAGACATCGTCTATACCTCCTGTC
  ATGATGCGGTCTATGAAACTCCCTTCTACGTAGCTGTGGACCATGACAAGAAGAAGGTGGTGATCAGTAT
  CCGGGGAACACTGTCCCCCAAGGATGCCCTGACTGACCTGACAGGAGATGCTGAGCGTCTCCCTGTGGAG
  GGACACCGAGGCACCTGGTTGGGCCACAAGGGCATGGTACTCTCAGCTGAGTACATCAAGAAGAAGCTGG
  AGCAGGAGATGGTCCTGTCCCAGGCCTTTGGGCGAGACCTGGGCCGTGGAACCAAACACTATGGCCTGAT
  TGTGGTAGGCCACTCCTTGGGAGCAGGCACAGCCGCCATTCTCTCCTTCCTCCTGCGCCCTCAGTACCCG
  ACCCTCAAGTGCTTCGCCTACTCCCCTCCAGGAGGCCTGCTGAGTGAGGATGCCATGGAGTACTCCAAAG
  AGTTTGTGACTGCTGTGGTTCTGGGCAAAGACCTTGTTCCCAGGATCGGCCTTTCCCAGCTCGAAGGTTT
  CCGCCGGCAGCTTCTGGATGTCCTGCAGCGAAGCACCAAGCCCAAATGGCGGATCATCGTGGGGGCCACC
  AAGTGTATCCCCAAGTCGGAGCTGCCTGAGGATCAGGTGGAAGTGACTGCCTTGGCCAGCACACGGCTCT
  GGACACACCCCAGTGATCTGACCATCGCCCTTTCAGCCAGCACCCCACTCTACCCACCTGGCCGTATCAT
  CCATGTGGTCCATAACCACCCTGCTGAACAGTGCTGCTGCTGTGAACAGGAAGAGCCCACATACTTTGCC
  ATCTGGGGTGACAACAAAGCCTTTAATGAGGTGATCATCTCACCAGCCATGCTCCATGAGCACCTGCCCT
  ACGTGGTCATGGAGGGGCTCAATAAGGTGCTGGAGAATTACAACAAGGGAAAGACAGCGCTGCTCTCTGC
  TGCAAAGGTCATGGTGAGCCCCACTGAGGTAGACCTCACTCCTGAGCTCATCTTCCAGCAACAGCCGCTG
  CCCACGGGGCCACCTCTGCCCACTGGGCTGGCTCTGGAGCTGCCTGCCACAGAGCATCGCAACAGCAGTG
  TCAGGAGCAAGTCTCAGTCTGAGATGAGTCTAGAGGGCTTCTCTGAAGGACGGCTGCTGTCCCCAGTGGC
  TGCTGCCTCAGCAGCCCGGCAAGACCCTGTAGAGCTGTTGCTGCTGTCCACCCAGGAACGACTGGCCGCG
  GAGCTGCAGTCCCGGCGGGCACCACTGGCCACTATGGAGAGCCTCTCAGACACTGAGTCACTGTACAGCT
  TTGACTCACGCCGTTCCTCAGGCTTCCGCAGCATCAGAGGCTCACCCAGCCTCCATGCAGTACTGGAGCG
  TGATGAGGGCCACCTGTTTTACATCGACCCAGCCATTCCAGAGGAGAACCCATCCCTCAGCTCCCGCACA
  GAGCTGCTGGCAGCTGACAGTCTGTCCAAGCACTCGCAGGACACCCAACCCCTGGAGGCAGCACTAGGCA
  GCGGGGGTGTGACTCCTGAGCGACCACCAAGTGCAGCCAATGACGAGGAGGAGGCAGCCGGTGGCAGTGA
  GGGTGGTGGGGTGGCCCCACGAGGAGAGCTGGCACTGCACAATGGGCGCCTGGGGGATTCGCCCAGCCCT
  CAGGTGCTAGAATTCGCTGAGTTCATTGACAGCCTCTTCAACCTGGACAGCAAGAGCAGCTCCTTCCAGG
  ACCTGTACTGCATGATGGTGCCTGAGAGCCCCACTAGTGACTACACAGAGGGCCCCAAGTCCCCCAGCCA
  ACAGGAAATCTTGCTTCGGGCTCAGTTTGAGCCCAATCTGGTACCCAAGCCCCCCAGGCTCTTTGCAGGA
  TCTGCCGAACCCTCCTCTGGCATCTCACTCTCACCCTCCTTCCCACTCAGCTCCTCAGGGGAGCTCATGG
  ACCTGACACCCACGGGCCTCAGCAGCCAGGAGTGTCTGGCCACAGACAAGATCCGGACTTCCACCCCCAC
  AGGCCATGGGGCCAGCCCTACCAAGCAGGATGACCTGGTCATCTCAGCACGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001005886
• Insert Size: 3135 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005886.1, NP_001005886.1
• RefSeq Size: 5568 bp
• RefSeq ORF: 3135 bp
• Locus ID: 309207
• Cytogenetics: 1q43