Manba (NM_001031655) Rat Untagged Clone

CAT#: RN204699

Manba (untagged ORF) - Rat mannosidase, beta A, lysosomal (Manba), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Manba
• Synonyms: MGC116230; MGC125196
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN204699 representing NM_001031655
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCTCCACCTGCTCTTTCTGTTGGCGCTGTGCGGGGCAGGCTGTATGGCCGCAGGTCCCAGCTACA
  GCCTGAGAGGGAGCTGGCGGGTGAGCAACGGGAATAGTTCCCTGGAGCTGCCGGCTACTGTCCCTGGCTA
  TGTGCACAGCGCCCTGCAGCAGCACGGGCTGATCCAGGATCCTTACTACAGATTTAATGACCTGAACTAC
  AGATGGATTTCCTTAGATAACTGGACCTACAGCACAGAATTTAAAATCCCCTTTAATCGCAGTGAATGGC
  AGAAGGTAAAGTTGATTTTTGACGGAGTCGATACAGTTGCAGAAATCCTGTTCAATAATGTCACTATTGG
  GAAGACAGACAACATGTTCACCAGATATAGCTTTGACGTCACCAATGTGGTCAAAGACGTGAACTCCCTA
  AAGCTGCGGTTCCAGTCAGCAGTGCAGTATGCTGAGTGTCAGAGCAAAGCTCACACTCAATACCGGGTGC
  CCCCAGAGTGCCCCCCAGTGGAACAGAAGGGTGAATGCCACGTCAACTTCATCCGAAAGGAGCAGTGCTC
  CTTCAGCTGGGACTGGGGGCCTTCCTTTCCCTCTCAAGGAATCTGGAAAGACGTTAGAATCGAAGCCTAT
  AACATCGCTCATCTGGATCACCTGACATTTTTGCCATTGTATGATAACACTTCTCAGGCGTGGACTATTG
  AAATAGAGGCTTCTTTTGATGTTGTCAGCACAAAGCCAGTTGGTGGTCAGGTGACTATAGCCATCCCCGA
  ACTGAAAACACAGCAGGCAAACCACATTGAACTTCAACATGGGCAAAGGATTGTTAAACTCCTTGTCAAA
  ATTAGGAAGGACGTCACCGTGGAGACCTGGTGGCCTCATGGACATGGGAACCAGACTGGGTACAACACGA
  CAATTCTCTTTGCGCTGGATGGAGGCTTAAAAATTGAAAAAGCAGCCAAGGTTTATTTCAGAACAGTGCA
  ACTTATAGAAGAACCTATAACAGGGTCTCCTGGTCTGAGTTTCTACTTCAAAATTAATGGGCTTCCTATA
  TTTCTGAAAGGCTCAAACTGGATCCCAGCTGACTCATTCCAAGACAAAGTCACTTCTGAACTGCTGCAGC
  TCCTTCTCCAGTCCGCCGTGGATGCTAACATGAACACTCTGCGCGTGTGGGGGGGTGGCATCTACGAGCA
  GGATGAATTCTATGCACTGTGTGACGAACTTGGAATAATGGTGTGGCAGGACTTCATGTTTGCCAGTGCC
  CTCTATCCAACTGAGCCAGGCTTCCTAGAGTCAGTGAGAAAAGAAGTCACTTACCAGGTCAGGAGACTGA
  AGTCTCACCCCTCCGTCATCATCTGGAGTGGTAATAATGAGAATGAAGTGGCTCTCAGGGTGAACTGGTT
  CCATGTAAACCCCAGGGACCTGGGGACTTACATCAACGACTATGTGACCCTCTACGTGAAAACCATCAGG
  GAGATTGTCTTATCGGAAGATAGGAGTCGTCCGTTCATTGCATCCAGCCCAACCAATGGGGTCAAAACCA
  TGACGGAAGGCTGGATCTCTAAGGACCCTTATAGCACCCAGTATGGCGACATGCATTTTTACGACTATTT
  CAGTGACTGCTGGGACTGGAAGGTCTTTCCAAAAGCTCGATTAGTGTCTGAATATGGCTACCAGTCCTGG
  CCTTCCTTCAGCACACTACAAAAGGTCTCTCGTCAAGAGGACTGGTCTTACAGCAGCCGCTTCTCCCTTC
  ATCGGCAGCATCATGGAAATGGCAACAATGAAATGCTTCACCAGGTACAGCTTCATTTCCAACTCCCCCA
  AAGGAGAGACCCAGTGCGTGCATTTAAAGATACCATCTACCTCACTCAGGTGATGCAGGCCCAGTGTATC
  AAAACAGAAACCGAGTTCTACCTGCGCAGCCGCAGCGAGATAGTGAATGGAGAAGGCCACACCATGGGGG
  CACTGTACTGGCAGCTGAATGATATCTGGCAGGCTCCTTCCTGGGCCTCTCTTGAGTACGGAGGGAAATG
  GAAAATGCTTCATTACTTCGCTCAGCGTTTCTTCGCTCCGCTGCTGCCAGTCGGTTTTGAAGATGAAGGC
  GTTTTTTATGTCTACGGTGTGTCAGATCTTCACAAGGACTACCCCACAAAGCTCACCGTGAGGCTCCACC
  GCTGGAGCTCCCAGAAGCCTTTGTGCACCTTCGTGAGTTTGAGTGCTGTGATTAAGGCAGGGGAGGCGAT
  GGTTCTGTTCCAGATGCCAGTGTCTAAGCTGCTGAAGAGGTGTAAAGAATGCACAAGGGACACCTGTGTG
  GTTTCCTTTTACCTCTCAACTGACAATGAACTCTTCAGTCCAACCAACTACCATTTCCTGTCCTCCCTGA
  AGGATGCCAAAGGGATGGTCAAGGCCAACATCACTGTCAGCATCTCTCAGAAGGGTGATCTATTTGTTTT
  TGATCTGAAATCGTCAACATCAGCCATCACTCCCTTTGTTTGGTTGGATGTAGGAAGCATCCCGGGGAGA
  TTTAGTGACAACGGCTTCCTCATGATTAAGAAGGAGCTCTCGGTACTGTTTTACCCATGGAAGCCCACCA
  GCAAGAGTGAGCTGCAGCAGGCCTTCAGTGTCACCTCCCTGACAGATCTGTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001031655
• Insert Size: 2646 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001031655.1, NP_001026825.1
• RefSeq Size: 3688 bp
• RefSeq ORF: 2646 bp
• Locus ID: 310864
• Cytogenetics: 2q43