Manba (NM_001031655) Rat Tagged ORF Clone

CAT#: RR204699

Manba (Myc-DDK-tagged ORF) - Rat mannosidase, beta A, lysosomal (Manba), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Manba
• Synonyms: MGC116230; MGC125196
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204699 representing NM_001031655
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCTCCACCTGCTCTTTCTGTTGGCGCTGTGCGGGGCAGGCTGTATGGCCGCAGGTCCCAGCTACA
  GCCTGAGAGGGAGCTGGCGGGTGAGCAACGGGAATAGTTCCCTGGAGCTGCCGGCTACTGTCCCTGGCTA
  TGTGCACAGCGCCCTGCAGCAGCACGGGCTGATCCAGGATCCTTACTACAGATTTAATGACCTGAACTAC
  AGATGGATTTCCTTAGATAACTGGACCTACAGCACAGAATTTAAAATCCCCTTTAATCGCAGTGAATGGC
  AGAAGGTAAAGTTGATTTTTGACGGAGTCGATACAGTTGCAGAAATCCTGTTCAATAATGTCACTATTGG
  GAAGACAGACAACATGTTCACCAGATATAGCTTTGACGTCACCAATGTGGTCAAAGACGTGAACTCCCTA
  AAGCTGCGGTTCCAGTCAGCAGTGCAGTATGCTGAGTGTCAGAGCAAAGCTCACACTCAATACCGGGTGC
  CCCCAGAGTGCCCCCCAGTGGAACAGAAGGGTGAATGCCACGTCAACTTCATCCGAAAGGAGCAGTGCTC
  CTTCAGCTGGGACTGGGGGCCTTCCTTTCCCTCTCAAGGAATCTGGAAAGACGTTAGAATCGAAGCCTAT
  AACATCGCTCATCTGGATCACCTGACATTTTTGCCATTGTATGATAACACTTCTCAGGCGTGGACTATTG
  AAATAGAGGCTTCTTTTGATGTTGTCAGCACAAAGCCAGTTGGTGGTCAGGTGACTATAGCCATCCCCGA
  ACTGAAAACACAGCAGGCAAACCACATTGAACTTCAACATGGGCAAAGGATTGTTAAACTCCTTGTCAAA
  ATTAGGAAGGACGTCACCGTGGAGACCTGGTGGCCTCATGGACATGGGAACCAGACTGGGTACAACACGA
  CAATTCTCTTTGCGCTGGATGGAGGCTTAAAAATTGAAAAAGCAGCCAAGGTTTATTTCAGAACAGTGCA
  ACTTATAGAAGAACCTATAACAGGGTCTCCTGGTCTGAGTTTCTACTTCAAAATTAATGGGCTTCCTATA
  TTTCTGAAAGGCTCAAACTGGATCCCAGCTGACTCATTCCAAGACAAAGTCACTTCTGAACTGCTGCAGC
  TCCTTCTCCAGTCCGCCGTGGATGCTAACATGAACACTCTGCGCGTGTGGGGGGGTGGCATCTACGAGCA
  GGATGAATTCTATGCACTGTGTGACGAACTTGGAATAATGGTGTGGCAGGACTTCATGTTTGCCAGTGCC
  CTCTATCCAACTGAGCCAGGCTTCCTAGAGTCAGTGAGAAAAGAAGTCACTTACCAGGTCAGGAGACTGA
  AGTCTCACCCCTCCGTCATCATCTGGAGTGGTAATAATGAGAATGAAGTGGCTCTCAGGGTGAACTGGTT
  CCATGTAAACCCCAGGGACCTGGGGACTTACATCAACGACTATGTGACCCTCTACGTGAAAACCATCAGG
  GAGATTGTCTTATCGGAAGATAGGAGTCGTCCGTTCATTGCATCCAGCCCAACCAATGGGGTCAAAACCA
  TGACGGAAGGCTGGATCTCTAAGGACCCTTATAGCACCCAGTATGGCGACATGCATTTTTACGACTATTT
  CAGTGACTGCTGGGACTGGAAGGTCTTTCCAAAAGCTCGATTAGTGTCTGAATATGGCTACCAGTCCTGG
  CCTTCCTTCAGCACACTACAAAAGGTCTCTCGTCAAGAGGACTGGTCTTACAGCAGCCGCTTCTCCCTTC
  ATCGGCAGCATCATGGAAATGGCAACAATGAAATGCTTCACCAGGTACAGCTTCATTTCCAACTCCCCCA
  AAGGAGAGACCCAGTGCGTGCATTTAAAGATACCATCTACCTCACTCAGGTGATGCAGGCCCAGTGTATC
  AAAACAGAAACCGAGTTCTACCTGCGCAGCCGCAGCGAGATAGTGAATGGAGAAGGCCACACCATGGGGG
  CACTGTACTGGCAGCTGAATGATATCTGGCAGGCTCCTTCCTGGGCCTCTCTTGAGTACGGAGGGAAATG
  GAAAATGCTTCATTACTTCGCTCAGCGTTTCTTCGCTCCGCTGCTGCCAGTCGGTTTTGAAGATGAAGGC
  GTTTTTTATGTCTACGGTGTGTCAGATCTTCACAAGGACTACCCCACAAAGCTCACCGTGAGGCTCCACC
  GCTGGAGCTCCCAGAAGCCTTTGTGCACCTTCGTGAGTTTGAGTGCTGTGATTAAGGCAGGGGAGGCGAT
  GGTTCTGTTCCAGATGCCAGTGTCTAAGCTGCTGAAGAGGTGTAAAGAATGCACAAGGGACACCTGTGTG
  GTTTCCTTTTACCTCTCAACTGACAATGAACTCTTCAGTCCAACCAACTACCATTTCCTGTCCTCCCTGA
  AGGATGCCAAAGGGATGGTCAAGGCCAACATCACTGTCAGCATCTCTCAGAAGGGTGATCTATTTGTTTT
  TGATCTGAAATCGTCAACATCAGCCATCACTCCCTTTGTTTGGTTGGATGTAGGAAGCATCCCGGGGAGA
  TTTAGTGACAACGGCTTCCTCATGATTAAGAAGGAGCTCTCGGTACTGTTTTACCCATGGAAGCCCACCA
  GCAAGAGTGAGCTGCAGCAGGCCTTCAGTGTCACCTCCCTGACAGATCTGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204699 representing NM_001031655
  Red=Cloning site Green=Tags(s)
  
  MHLHLLFLLALCGAGCMAAGPSYSLRGSWRVSNGNSSLELPATVPGYVHSALQQHGLIQDPYYRFNDLNY
  RWISLDNWTYSTEFKIPFNRSEWQKVKLIFDGVDTVAEILFNNVTIGKTDNMFTRYSFDVTNVVKDVNSL
  KLRFQSAVQYAECQSKAHTQYRVPPECPPVEQKGECHVNFIRKEQCSFSWDWGPSFPSQGIWKDVRIEAY
  NIAHLDHLTFLPLYDNTSQAWTIEIEASFDVVSTKPVGGQVTIAIPELKTQQANHIELQHGQRIVKLLVK
  IRKDVTVETWWPHGHGNQTGYNTTILFALDGGLKIEKAAKVYFRTVQLIEEPITGSPGLSFYFKINGLPI
  FLKGSNWIPADSFQDKVTSELLQLLLQSAVDANMNTLRVWGGGIYEQDEFYALCDELGIMVWQDFMFASA
  LYPTEPGFLESVRKEVTYQVRRLKSHPSVIIWSGNNENEVALRVNWFHVNPRDLGTYINDYVTLYVKTIR
  EIVLSEDRSRPFIASSPTNGVKTMTEGWISKDPYSTQYGDMHFYDYFSDCWDWKVFPKARLVSEYGYQSW
  PSFSTLQKVSRQEDWSYSSRFSLHRQHHGNGNNEMLHQVQLHFQLPQRRDPVRAFKDTIYLTQVMQAQCI
  KTETEFYLRSRSEIVNGEGHTMGALYWQLNDIWQAPSWASLEYGGKWKMLHYFAQRFFAPLLPVGFEDEG
  VFYVYGVSDLHKDYPTKLTVRLHRWSSQKPLCTFVSLSAVIKAGEAMVLFQMPVSKLLKRCKECTRDTCV
  VSFYLSTDNELFSPTNYHFLSSLKDAKGMVKANITVSISQKGDLFVFDLKSSTSAITPFVWLDVGSIPGR
  FSDNGFLMIKKELSVLFYPWKPTSKSELQQAFSVTSLTDLY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001031655
• ORF Size: 2643 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001031655.1, NP_001026825.1
• RefSeq Size: 3688 bp
• RefSeq ORF: 2646 bp
• Locus ID: 310864
• Cytogenetics: 2q43
• MW: 101.2 kDa