C1qa (NM_001008515) Rat Untagged Clone

CAT#: RN205235

C1qa (untagged ORF) - Rat complement component 1, q subcomponent, alpha polypeptide (C1qa), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: C1qa
• Synonyms: MGC105755
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN205235 representing NM_001008515
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCTCTCAGGGATGGCTGGTGGCTTGTGTGCTGGCCGTGACCCTGGTATGGACAGTGGCTGAAG
  ATGTCTGCAGAGCACCCAACGGGAAGGACGGGGTTGCAGGAATTCCTGGCCGCCCAGGGAGGCCGGGTCT
  CAAAGGAGAGAGAGGGGAGCCAGGAGCTGCCGGCATCCGGACCGGTATCCGAGGTCTTAAAGGAGACATG
  GGGGAATCTGGGCCCCCTGGCAAACCCGGCAATGTGGGGTTCCCAGGGCCCACTGGGCCCCTGGGGAACA
  GCGGCCCCCAAGGGTTGAAAGGTGTGAAAGGCAATCCGGGCAATATCAGGGACCAGCCCCGGCCAGCTTT
  CTCAGCTATTCGGCAGAACCCACCGACGTATGGCAACGTGGTTGTCTTTGACAAGGTCCTCACCAACCAG
  GAGAATCCATACCAGAACCGCACAGGTCACTTCATCTGTGCGGTGCCCGGTTTCTATTACTTCACCTTCC
  AAGTGATCTCCAAGTGGGACCTTTGTCTGTCTATCGTGTCCTCCTCCCGGGGCCAGCCCAGGAATTCCCT
  TGGTTTCTGTGACACCAACAGCAAGGGGCTCTTCCAGGTGTTAGCAGGGGGCACTGTGCTTCAATTGCAA
  CGAGGGGACGAGGTGTGGATTGAGAAGGACCCAGCAAAGGGCCGCATTTACCAGGGTACTGAAGCCGACA
  GCATCTTCAGTGGATTCCTCATTTTTCCCTCGGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001008515
• Insert Size: 738 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008515.1, NP_001008515.1
• RefSeq Size: 1025 bp
• RefSeq ORF: 738 bp
• Locus ID: 298566
• Cytogenetics: 5q36
• Gene Summary: C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes. [UniProtKB/Swiss-Prot Function]