Enpp7 (NM_001012466) Rat Untagged Clone

CAT#: RN205492

Enpp7 (untagged ORF) - Rat ectonucleotide pyrophosphatase/phosphodiesterase 7 (Enpp7), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Enpp7
• Synonyms: E-NPP 7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN205492 representing NM_001012466
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCACTCAGCTGTCCTCCTCTCTGTGGCTCTAGTCATCCTCCCAGCTTGTGTGACTGGGGGTCCTG
  TCCAAAGGCAGCAGCAGCATAAGCTTCTCCTTGTATCCTTCGATGGCTTCCGCTGGAACTATGACCAAGA
  CGTGGAAACCCCTAACCTGGACTCCATGGCTCAGGAAGGAGTGAAAGCTCGCTACATGACTCCTGCCTTT
  GTCACCATGACCAGCCCCTGCCACTTTACACTGGTCACCGGCAAATACATCGAGAACCATGGGGTGGTCC
  ACAACATGTTCTATAACACCACCAACAAGGTGAGGCTGCCCTACCATGCCACGCTTGGGATCCAGAGGTG
  GTGGGATAACGGCAGCATACCCATCTGGATCACAGCCCAGAGGCAGGGCTTAAAAACCGGCTCCTTCTTC
  TACCCTGGTGGGAACGTCACCTACCAAGGAGAGGCCGTGACGATGAGCCGGAAGGAAGGCGTCTTACACA
  ATTACAAAAACGAGACAGAGTGGAGGGCAAATGTGGACACAGTGATGAAGTGGTTCACGGAGGAGGATGT
  GTCCCTGGTCACTCTCTACTTTGGGGAGCCAGACTCCACTGGCCACAAGTATGGCCCTGAGTCCCAGGAG
  AGGAAGGACATGGTGAAACAGGTAGACAGGACGGTGGGCTACCTCCGGGACAGCATCAAGCGCCACCACC
  TCACAGACAGCCTCAACCTGATAATCACCTCGGACCACGGCATGACGACCGTCAACAAGAAGGCCAGCGA
  CTTGGTCGAGTTCCACAAGTTCCCCAACTTCACCTTCAGGGACATTGAGTTCGAACTCTTGGACTACGGG
  CCCAACGGGATGCTGATCCCCAAGGAAGGGATGCTGGAGAAGGTATACAGTGTCCTCAAGGATGCCCACC
  CCAGGCTGCATGTGTACAAGAAGGAGGACTTCCCGAAGACCTTTCACTACGCCAACAACCCCAGGATCAC
  ATCCCTGCTCATGTACAGTGACCTCGGCTATGTCATTCATGGGAGAGTGAACGTCCAGTTCAACAGTGGT
  GAGCATGGTTTCGACAACCAAGACATGGACATGAAGACCATCTTCCGGGCTGTGGGTCCCAGCTTCAAGG
  CAGGCTTGGAAGTGGAGCCCTTCGAGAGCGTCCATGTGTACGAGCTCATGTGTCAGCTGCTGGGCATTGT
  GCCCGAGCCCAACGATGGGCATCCAGGCGTCCTGCAACCCATGCTCCGGTCAGGATCTCCGCTCAGCAGG
  CAGCACCACTTGGTGGTGGTGTTGATGGGGATCCTAACGGGTCTGGCCAAGGTCGTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001012466
• Insert Size: 1320 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012466.1, NP_001012484.1
• RefSeq Size: 1320 bp
• RefSeq ORF: 1320 bp
• Locus ID: 303729
• Cytogenetics: 10q32.3
• Gene Summary: Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity. [UniProtKB/Swiss-Prot Function]