Enpp7 (NM_001012466) Rat Tagged ORF Clone

CAT#: RR205492

Enpp7 (Myc-DDK-tagged ORF) - Rat ectonucleotide pyrophosphatase/phosphodiesterase 7 (Enpp7), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Enpp7
• Synonyms: E-NPP 7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205492 representing NM_001012466
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCACTCAGCTGTCCTCCTCTCTGTGGCTCTAGTCATCCTCCCAGCTTGTGTGACTGGGGGTCCTG
  TCCAAAGGCAGCAGCAGCATAAGCTTCTCCTTGTATCCTTCGATGGCTTCCGCTGGAACTATGACCAAGA
  CGTGGAAACCCCTAACCTGGACTCCATGGCTCAGGAAGGAGTGAAAGCTCGCTACATGACTCCTGCCTTT
  GTCACCATGACCAGCCCCTGCCACTTTACACTGGTCACCGGCAAATACATCGAGAACCATGGGGTGGTCC
  ACAACATGTTCTATAACACCACCAACAAGGTGAGGCTGCCCTACCATGCCACGCTTGGGATCCAGAGGTG
  GTGGGATAACGGCAGCATACCCATCTGGATCACAGCCCAGAGGCAGGGCTTAAAAACCGGCTCCTTCTTC
  TACCCTGGTGGGAACGTCACCTACCAAGGAGAGGCCGTGACGATGAGCCGGAAGGAAGGCGTCTTACACA
  ATTACAAAAACGAGACAGAGTGGAGGGCAAATGTGGACACAGTGATGAAGTGGTTCACGGAGGAGGATGT
  GTCCCTGGTCACTCTCTACTTTGGGGAGCCAGACTCCACTGGCCACAAGTATGGCCCTGAGTCCCAGGAG
  AGGAAGGACATGGTGAAACAGGTAGACAGGACGGTGGGCTACCTCCGGGACAGCATCAAGCGCCACCACC
  TCACAGACAGCCTCAACCTGATAATCACCTCGGACCACGGCATGACGACCGTCAACAAGAAGGCCAGCGA
  CTTGGTCGAGTTCCACAAGTTCCCCAACTTCACCTTCAGGGACATTGAGTTCGAACTCTTGGACTACGGG
  CCCAACGGGATGCTGATCCCCAAGGAAGGGATGCTGGAGAAGGTATACAGTGTCCTCAAGGATGCCCACC
  CCAGGCTGCATGTGTACAAGAAGGAGGACTTCCCGAAGACCTTTCACTACGCCAACAACCCCAGGATCAC
  ATCCCTGCTCATGTACAGTGACCTCGGCTATGTCATTCATGGGAGAGTGAACGTCCAGTTCAACAGTGGT
  GAGCATGGTTTCGACAACCAAGACATGGACATGAAGACCATCTTCCGGGCTGTGGGTCCCAGCTTCAAGG
  CAGGCTTGGAAGTGGAGCCCTTCGAGAGCGTCCATGTGTACGAGCTCATGTGTCAGCTGCTGGGCATTGT
  GCCCGAGCCCAACGATGGGCATCCAGGCGTCCTGCAACCCATGCTCCGGTCAGGATCTCCGCTCAGCAGG
  CAGCACCACTTGGTGGTGGTGTTGATGGGGATCCTAACGGGTCTGGCCAAGGTCGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205492 representing NM_001012466
  Red=Cloning site Green=Tags(s)
  
  MGHSAVLLSVALVILPACVTGGPVQRQQQHKLLLVSFDGFRWNYDQDVETPNLDSMAQEGVKARYMTPAF
  VTMTSPCHFTLVTGKYIENHGVVHNMFYNTTNKVRLPYHATLGIQRWWDNGSIPIWITAQRQGLKTGSFF
  YPGGNVTYQGEAVTMSRKEGVLHNYKNETEWRANVDTVMKWFTEEDVSLVTLYFGEPDSTGHKYGPESQE
  RKDMVKQVDRTVGYLRDSIKRHHLTDSLNLIITSDHGMTTVNKKASDLVEFHKFPNFTFRDIEFELLDYG
  PNGMLIPKEGMLEKVYSVLKDAHPRLHVYKKEDFPKTFHYANNPRITSLLMYSDLGYVIHGRVNVQFNSG
  EHGFDNQDMDMKTIFRAVGPSFKAGLEVEPFESVHVYELMCQLLGIVPEPNDGHPGVLQPMLRSGSPLSR
  QHHLVVVLMGILTGLAKVV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012466
• ORF Size: 1317 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012466.1, NP_001012484.1
• RefSeq Size: 1320 bp
• RefSeq ORF: 1320 bp
• Locus ID: 303729
• Cytogenetics: 10q32.3
• MW: 49.8 kDa
• Gene Summary: Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity. [UniProtKB/Swiss-Prot Function]