F12 (NM_001014006) Rat Untagged Clone

CAT#: RN205503

F12 (untagged ORF) - Rat coagulation factor XII (Hageman factor) (F12), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: F12
• Synonyms: HAF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN205503 representing NM_001014006
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGCTCTGTTGTTCCTGGGGTCTCTGCTGATGAGTCTGGACTTGACACTTTCGGCGCCACCGTGGA
  AGTCCAAGGAGTTCAAGGACGGAGCTGGCGATCCCTCTGTGGTTCTCACTGTGGACGGGAAGCTCTGCCA
  CTTTCCCTTTCAGTACCACCGTCGCCTGTACCACAAATGCATCCACAAAGGACAGCCAGGCTCCAGGCCC
  TGGTGTGCTACCACCCCCAACTTTGACGAGGACCAGCAATGGGGATACTGCTTGGAGCCCAAGAAAGTGA
  AAGACCATTGCAGCAAACACAGCCCCTGCCACAAAGGAGGGACGTGTGTCAACACCCCCAACGGCCCGCA
  CTGTCTCTGCCCTGAACACCTCACCGGGAAACATTGCCAGAGAGAGAAATGCTTTGAGTCTCAGCTCCTC
  AAGTTCTTCCATGAGAATGAGATATGGTTTAGAACTGGGCCAGGAGGTGTGGCCAGGTGCCAGTGCAAAG
  GTCCTCAGGCTGTTTGCAAGCTGCTGACCAGTCAGGTTTGCAGGGTCAATCCGTGCCTTAATGGAGGCAC
  CTGCCTCCTCGTGGAGGACCACCGACTGTGCCACTGCCCTGCAGGCTATGCCGGACCTTTTTGCGACTTA
  GACCTTAAGGCGACTTGCTACGAAGACAGGGGTCTCAGCTACCGGGGCCAGGCTAAAACTACTCTGTCGG
  GTGCACCATGTCAGCGGTGGGCCTCGGAGGCCACCTACCGGAACATGACTGAGACGCAAGCTCTAAGCTG
  GGGCCTGGGCCACCACGCATTCTGCCGGAACCCAGATAATGACACACGTCCATGGTGCTACGTCTGGAGT
  GGCGACAGGCTGAGCTGGGACTACTGCGACCTGGAACAGTGCCAGATGCCAACGCTCACATCTCCGGTTT
  CCCCTGAGAGTCACGACATGCTGAAGCCCCGGCCTCCCATATTGCAGATGCCTCAGTTCCCGTCTCTGTC
  CGATGCACTAGACAACTCGACCCGTAATCAGAATGTTGTGTCCAGGACCAGTACGGTGGTCTGCGGACAG
  AGGTTTCGCAAGCGACTGTCCTCGCTCAGGCGCGTGGTGGGCGGACTAGTGGCTCTGCCTGGATCGCATC
  CCTACATCGCTGCACTGTACTGGGGCGACAGCTTCTGCGCAGGCAGTCTCATCGACCCCTGCTGGGTGCT
  GACCGCTGCTCACTGCTTGCAGAAACGGCCAGCGCCCGAGGAACTGACAGTGGTACTTGGTCAAGATCGC
  CATAACCAGAGCTGCGAGAGGTGCCAGACTCTGGCTGTGCACTCCTACCGCCTTCACGAGGGCTTCTCTT
  CCAAAACCTACCAGCATGATTTGGCTCTGCTGCGCCTGCGGGGGAGGAAAAACAGCTGCGCGATCTTGTC
  GCCTCATGTCCAGCCGGTGTGTCTGCCCAGCAGCGCGGCCCCACCCTCTGAGACAGTGCTCTGCGAGGTG
  GCCGGCTGGGGTCATCAGTTCGAGGGGGCTGAAGAATACGCCACCTTTCTGCAGGAGGCACAGGTACCCT
  TCATCTCCCTGGATCGCTGCTCCAGCTCTAACGTGCACGGAGACGCCATCCTGCCTGGGATGCTTTGTGC
  TGGCTTCTTGGAGGGAGGCGCCGATGCCTGTCAGGGTGACTCCGGGGGTCCTCTGGTATGTGATGAAGGA
  GTTACAGAGCGTCAGCTCACCCTGCGAGGAGTCATCAGCTGGGGCTCCGGCTGTGGTGACCGGAACAAGC
  CCGGGGTCTACACTGACGTGGCCAATTACCTGGATTGGATCCAGGAGCATACTGCTTTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001014006
• Insert Size: 1812 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014006.1, NP_001014028.1
• RefSeq Size: 2128 bp
• RefSeq ORF: 1812 bp
• Locus ID: 306761
• Cytogenetics: 17p14