Arhgef12 (NM_001013246) Rat Untagged Clone

CAT#: RN205717

Arhgef12 (untagged ORF) - Rat Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Arhgef12
• Synonyms: Larg
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN205717 representing NM_001013246
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGCACACAGTCTACTATCACGGACAGGTTTCCCCTCAAAAAACCTATAAGGCATGGAAGTATTT
  TGAACCGAGAGTCACCAACAGATAAGAAACAGAAAGTCGAACGCAGCACATCACATGATTTTGACCCCAC
  AGATAGCTCCTCCAAGAAGACAAAGTCTAGTTCAGAGGAGAGTAGATCCGAGATCTATGGTCTTGTTCAG
  CGCTGTGTGATCATCCAGAAAGATGACAATGGATTCGGGCTGACAGTCAGCGGGGACAATCCAGTCTTCG
  TACAATCTGTCAAAGAAGATGGAGCAGCCATGCGCGCTGGAGTACAAACAGGTGATCGAATCATCAAGGT
  GAATGGGACTCTGGTGACTCATTCAAATCATCTGGAGGTGGTGAAACTAATCAGATCTGGTTCCTATGTA
  GCTCTCACTGTCCAGGGACGCCCACCTGGGTCGCCCCAGATTCCACTTGCTGAGTCTGAAGTAGAACCAT
  CAGTCATTGGACATATGTCTCCCATTATGACATCCCCTCATTCCCCTGGAGCAGCCGGGAACATGGAGAG
  AATCACTAGTCCTGTGCTCATGGGGGAGGAAAACAATGTGGTCCATAACCAGAAAGTAGAGATTCTGAGG
  AAAATGTTACAGAAAGAACAGGAGCGTCTGCAGTTACTGCAGGAAGATTACAACCGAACGGCTACCCAGA
  GATTGCTCAAGGAGATCCAGGAGGCCAAGAAGCACATCCCCCAGCTGCAGGAGCAGCTGTCCAAAGCCAC
  AGGCTCTGCTCAGGACGGAGCTGTAATTGCACCCTCCAGGCCATTAGGTGATGCCCTAACACTCAGCGAG
  GCAGAAACGGACCCTGGAGACGGCCTGTGCAGGACTGACTGGAGCAGTGGAGATGCTTCTCGGCCCAGTA
  GTGACAGTGCAGATAGCCCCAAGAGTAGCCTGAAGGAAAGGGTTTGTCTCGAGGACACCCCAGAGAGGAC
  TGAAGGAGTTCAGGATGCTGACACACAGTCGCTGGTCGGCAGTCCCTCCACCCGTGGCGCCCCTCATATT
  ATCGGAGCAGAGGATGATGATTTTGGTACTGAACACGAGCAGATCAATGGACAGTGCAGCTGTTTCCAGA
  GCATTGAGTTGCTGAAGTCTCGCCCTGCCCACTTGGCTGTTTTCTTACACCATGTAGTCTCACAGTTTGA
  CCCTGCAACATTGCTCTGTTATCTCTATTCAGACCTGTATAAGCAGACGAACTCCAAGGAGACTCGGCGG
  GTCTTCCTGGAGTTTCATCAGTTCTTCTTGGACCGATCTGCACACCTGAGAGTTTCCGTCCCCGAGGAAA
  TCTCTGTGGATCTAGAAAAGAGAAGACCCGAGCTCATTCCTGAGGATCTGCATCGCCATTACATTCAAAC
  AATGCAGGAGAGGGTTCACCCTGAAGTTCAGAGGCACTTGGAGGATTTTCGACAGAAACGCAGCATGGGA
  CTGACCTTGGCTGAGAGTGAGCTGACCAAACTTGATGCAGAGAGAGACAAGGACCGGGGAACTCTGGAGA
  AGGAGCGAGCATGTGCGGAACAGATCGTTACCAAAATTGAAGAAGTGTTGATGACTGCTCAGGCTGTAGA
  AGAAGAAAGGAGTTCCACAATGCAGTATGTAATTCTCATGTATATGAAGTATTTGGGAGTAAAAGTGAAA
  GAACCTCGAAACTTGGAGCACAAGCGGGGTCGGATCGGCTTCCTTCCAAAGATCAAGCAAAGCATGAAGA
  AAGATAGAGAGGGCGAAGAAAAAGGGAAGCGAAGAGGATTCCCCAGCATTTTGGGACCCCCAAGGAGACC
  AAGCCGCCACGACAACAGTGCAATTGGCAGGGCCATGGAGATACAGAAGCTTCGCCACCCGAAGCACTTG
  TCCGCACCCTCATCTATGAGTCCTGAGCTTCAGGACCCTGCTAAGTTGCGCCAGAGTGGAGTAGCCAATG
  AAGGAACAGACACTGGATATCTGCCTGCCAGTTCCATGTCCTCTGCCACCTCAGGGGCTGCTCTTTCCCA
  AGAGGGAGGGAGAGAGAATGACACAGGAGCAAAACAAGTTGGAGACGCGGTGGCACCTGGAGACTGCTTG
  GATAGCATACCTCGAGTCCCCAATACTGTTTTTGACTTCCCCCCGCCTCTGCTAGATCAAGTGCAGGAGG
  AGGAATGTGAAGTGGAGAGGGTGGCAGAACATGGAACCCCAAAACCCTTTCGAAAGTTTGACAGCATCGC
  CTTTGGAGAAAGTCAGAGTGAGGACGAGCAGTTTGAAAATGACCTGGAAACAGATCCACCCAACTGGCAG
  CAGCTCGTTAGCAGAGAAGTGCTACTGGGACTGAAACCCTCCGAGATCAAGAGACAGGAAGTGATTAATG
  AATTGTTCTACACTGAGAGAGCGCATGTCCGAACACTGAAGGTTCTTGACCAAGTATTCTATCAGCGAGT
  GTCCAGGGAAGGGATTCTATCACCTTCCGAGCTGCGCAAGATTTTTTCAAACTTGGAGGATATCCTTCAA
  CTTCATGTTGGGTTGAATGAGCAGATGAAAGCTGTCCGAAAAAGGAACGAGACCTCAGTTATTGATCATA
  TTGGGGAAGATCTGCTCCTTTGGTTCAGTGGACCAGGGGAGGAGAAGCTGAAGCACGCTGCTGCCACATT
  CTGCAGTAACCAGCCTTTCGCCCTGGAAATGATCAAGTCTCGCCAGAAAAAGGATTCTCGATTCCAGACT
  TTTGTGCAAGATGCTGAGAGTAACCCCCTGTGCCGTCGTCTTCAGCTGAAGGATATCATTCCCACCCAAA
  TGCAGAGGCTCACCAAGTACCCCCTTCTTTTGGATAATATCGCCAAATACACAGAATGGCCTCCAGAAAG
  GGAAAAGGTGAAGAAGGCTGCAGATCAATGTCGTCAGATCTTAAATTATGTAAATCAGGCTGTCAGGGAG
  GCAGAAAACAAGCAGCGCTTAGAAGACTATCAGCGTCGCCTTGACACCTCCAGTCTCAAGTTGTCTGAGT
  ACCCGAATGTTGATGAGCTCAGGAATTTGGATTTAACAAAAAGAAAGATGATTCATGAAGGGCCGTTGGT
  CTGGAAGGTGAATAGAGATAAAAGCATCGATCTGTATACACTGCTGCTGGAAGACATTCTTGTGCTGTTA
  CAGAAGCAGGACGACAGGCTGGTACTGAGGTGCCACAGCAAGATCCTGGCCTCCACAGCTGACAGCAAGC
  ACACCTTCAGTCCTGTCATTAAGCTCAGCACAGTGCTGGTCCGACAGGTGGCCACAGATAACAAAGCTTT
  ATTCGTCATCTCCATGTCAGATAATGGAGCCCAAATTTATGAATTGGTAGCACAGACGGTTTCTGAAAAG
  ACGGTCTGGCAAGACCTCATCTGTCGGATGGCCGCATCAGTGAAGGAGCAGTCCACAAAGCCGATTCCCC
  TCCCACAGCCGCCACCTTGCGAAGGAGACAATGATGAAGAAGAACCAGCCAAGTTAAAAGTGGAGCACCA
  TGATCTTTCTGTCGCTGGTCTGCAGAGTCCAGACAGAGTTTTGGGATTAGAATCTCCCTTAATTTCGTCA
  AAACCTCAGTCCCATTCAATGAATACCCCTGGCAAATCAGAGGCAGAGGCAGAACATCTCTTTGTGACCG
  CAACACAGTTTGCAAAGGAGCAGCATGCAAATGGGACCTTCAAGGAAGGTGATGGCGGGTATCCAGTCAC
  AATCTCAGATCCACACTTGCCCGTGTCAGAGGAGCGGTGGGCATTGGACGCACTGAGAAATTTGGGCTTG
  TTGAAGCAGTTGCTGGTTCAGCAGTTGGGGCTCACGGAGAAGACTACTCAGGAAGACTGGCGGTCCTTCT
  CAAGATACAGACCGGCTTCTGAAGAGGCACAGGCGGACAGTGGCATTCCGGACTTGGAAAGTGTTAAGGC
  TTGTCATGCCGGGGAAGGACAGATGTCTTTTAAAACTGGAACTGGTGATGTTGCAACTTGTGACAGTCCA
  CGGACTTCAACTGAGTCCTGTGCTGTACAGGATTCGGTGATACTGGCTTCCCAAGACAGCCAGGCAAGCA
  ACATCATAGAAATGGACCACATGCTCCTGACCCCAGAGGTGCCTACTACTGCAGAGCCAGAGGGGGGTCT
  GGATGACAGTGGAGAGCACTTTTTTGATGCCCGTGAAGCACATAGCGATGATAATCCATCTGAAGGTGAT
  GGCGCAGTCAGAAAGGAAGAGGAGGATGTTAATGTGCGCATCTCAGGAAACTGTTTGATCCTTGATGGCT
  ATGATGCAGTGCAGGAGAGCTCCACAGATGAGGAGATTGCCTCCTCATTCACCCTGCAGCCTGTGACAGG
  CCTCCCTTCTGTGGACTCCAGCCACCAGCAGCAACACTCCCCTCAGAATGCCATTTCCGAGGGGGCGATT
  TCACCATCCACCCCAGAATTCCTGGTCCAGCGACGCTGGAGAGCTGTGGATGCTTGCTCTGAGGTCCAGA
  GTCCCTCTGCTTGGACAGATTCACAAAGCCAAATCCTGGAGTACATTCATAAAATAGAGGCTGACCTGGA
  ACACTTAAAGAAGGTGGAGGAAAGTTACACCCTTCTTTGCCAAAGGCTGGCTGGCTCAGCCCTCCCCGAC
  AAGGTCTCAGATAAAAGTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001013246
• Insert Size: 4641 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013246.1, NP_001013264.1
• RefSeq Size: 4668 bp
• RefSeq ORF: 4641 bp
• Locus ID: 367072
• Cytogenetics: 8q22