Trim25 (NM_001009536) Rat Untagged Clone

CAT#: RN206584

Trim25 (untagged ORF) - Rat tripartite motif-containing 25 (Trim25), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Trim25
• Synonyms: Efp; Znf147
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN206584 representing NM_001009536
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGCTGTTTCCTCTGGCCGAGGAGCTGTCGTGCTCCATCTGCCTGGAGCCCTTCAAGGAGCCCG
  TCACCACCCCGTGCGGCCACAATTTCTGCAGGTCATGCCTAAATGAGACGTGGGTCGTCCAGGGCCCGCC
  GTACCGGTGCCCGCAGTGTCGCACCGTGTACCAGGTGCGCCCGCAGCTGCACAAGAACACGGTGCTGTGC
  GCAGTGGTCGAGCAGTTTCTTCAGGCCGAGCAGGCGCGGACCCCCGTGGACGACTGGATCCCGCCTACCC
  GCTCCGCCGCATCCAGCTCAGACACCCAGGTGTCCTGCGACCACTGCCTGAAGGAGGTCGCGGTGAAGAC
  GTGCCTCGTGTGCATGGCCTCTTTCTGCCAGGAGCACTTGCGGCCTCACATCGACAGTCCGGCCTTCCAG
  GACCACCCCCTGCAGCCACCCGTCCGTGACCTGCTGCGCCGCAAATGTTCCAGGCACAACCGCCTTCGCG
  AAATGTTTTGTCCTGAGCACGGAGAGTGCATCTGCCATATCTGCCTGGTAGAGCACAAGACCTGCTCCCC
  TACGATCCTAAGCCAGGCCAGCGCCGACCTGGAGAACAAACTGAGGCAAAAACTCACCATCATGTACAGC
  CACATCAATGGAGCGAGCAAAGCACTGGAGGATGTGAAATCCAAGCAGCAGAATGTGCAGGAGTCTATGA
  AGAGGAAGATGCTACAGCTGAGACAGGAATATTTGGAAATGAAGGCTGTCATCGATGCCGCAGAGACCAG
  CTCCACGCACAAGTTGAAGGAGGAGGAGAAGAGAGTCTATGGAAAGCTCGATACCATCTACCAGGTTCTT
  CTCAAGAAGAAGAACGAGATGCAGAATCTGAAGGCAGAGGTTGAGCTCATCCTGGCCAAAGGGGACGAGT
  TTGAGTTTCTGGAGAAAGCTGCAAAATTGCAGGAAGTGTCAACGAAACCAGTCTACATCCCTAAGATAGA
  CCTGGACCATGATTTGATAAAGGGGGTCTACCAGGGTGCATTGGACCTCAAGAGTGAGCTGAAGCTCTCC
  CTCAAGCAGCTACAGGAAAAGAAGGCTGAGGAGCCCTGCAACTCAGGTAACGTGGGAGACCATGCGCAGT
  CCACATCCAAACCCACACGCCCTGCGAAGAAGACCGCACCGGACAAGAAGACCAAGAAAACCCCTGGTGC
  TGCGGGTGCTGCCGGTGCTGCCGGTGCTGCTGGTGCTGCCGGTCCTACCTCCTACTCTTCCTCATCTAAC
  AAGTTGCCCACCTTTGGAGCTCCTGGACAAGCAACTATCCCTGATGCCACACTCAAACCCAATGCTCCAC
  AACCAAACTCGGCAGCCGTCAAGGCTAAGGTGTTAGAAAACTTCTTAACCATGTCCAGAACAGAGCTTCT
  GGAATATTTCGTTAAAGTCGTCTTCGACTACAATACCGCCCACAACAAAGTGGCTCTGGCAGACAACTAC
  ACCACCGCTTCCGTGGCCGACGGGCTGCAGCACTACCGGTCCCATCCCCAGAGGTTCACCTACTGCTCTC
  AGGTCCTGGGGCTGCACTGCTATAAGAAAGGCATCCACTACTGGGAGGTGGAGCTGCAGAAGAACAACTT
  CTGTGGTGTAGGCGTCTGCTATGGCAGCATGGATCGGCAGGGCCCCGAGAGCCGGCTGGGCCGTAACCCC
  AGCTCATGGTGTGTGGAGTGGTTCAATAACAAGATCTCCGCCTGGCACAACAACATGGAGAAAACGCTGC
  CCTCCACCAAGGCCACGCGAGTCGGCGTGCTACTCAACTGTGACCATGGCTTTGTCATTTTCTTTGCTGT
  CACCGAAAAGGTGTATCTGATGTACAAGTTCAAGGTGGACTTTACTGAAGCTCTGTACCCAGCCTTTTGG
  GTGTTCTCTGCGGGTACCACCCTCTCTATCTGCTCCAAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001009536
• Insert Size: 1932 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009536.1, NP_001009536.1
• RefSeq Size: 2447 bp
• RefSeq ORF: 1932 bp
• Locus ID: 494338
• Cytogenetics: 10q26
• Gene Summary: may play a role in osteoblast differentiation [RGD, Feb 2006]