Trim25 (NM_001009536) Rat Tagged ORF Clone

CAT#: RR206584

Trim25 (Myc-DDK-tagged ORF) - Rat tripartite motif-containing 25 (Trim25), (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Trim25
• Synonyms: Efp; Znf147
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206584 representing NM_001009536
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGCTGTTTCCTCTGGCCGAGGAGCTGTCGTGCTCCATCTGCCTGGAGCCCTTCAAGGAGCCCG
  TCACCACCCCGTGCGGCCACAATTTCTGCAGGTCATGCCTAAATGAGACGTGGGTCGTCCAGGGCCCGCC
  GTACCGGTGCCCGCAGTGTCGCACCGTGTACCAGGTGCGCCCGCAGCTGCACAAGAACACGGTGCTGTGC
  GCAGTGGTCGAGCAGTTTCTTCAGGCCGAGCAGGCGCGGACCCCCGTGGACGACTGGATCCCGCCTACCC
  GCTCCGCCGCATCCAGCTCAGACACCCAGGTGTCCTGCGACCACTGCCTGAAGGAGGTCGCGGTGAAGAC
  GTGCCTCGTGTGCATGGCCTCTTTCTGCCAGGAGCACTTGCGGCCTCACATCGACAGTCCGGCCTTCCAG
  GACCACCCCCTGCAGCCACCCGTCCGTGACCTGCTGCGCCGCAAATGTTCCAGGCACAACCGCCTTCGCG
  AAATGTTTTGTCCTGAGCACGGAGAGTGCATCTGCCATATCTGCCTGGTAGAGCACAAGACCTGCTCCCC
  TACGATCCTAAGCCAGGCCAGCGCCGACCTGGAGAACAAACTGAGGCAAAAACTCACCATCATGTACAGC
  CACATCAATGGAGCGAGCAAAGCACTGGAGGATGTGAAATCCAAGCAGCAGAATGTGCAGGAGTCTATGA
  AGAGGAAGATGCTACAGCTGAGACAGGAATATTTGGAAATGAAGGCTGTCATCGATGCCGCAGAGACCAG
  CTCCACGCACAAGTTGAAGGAGGAGGAGAAGAGAGTCTATGGAAAGCTCGATACCATCTACCAGGTTCTT
  CTCAAGAAGAAGAACGAGATGCAGAATCTGAAGGCAGAGGTTGAGCTCATCCTGGCCAAAGGGGACGAGT
  TTGAGTTTCTGGAGAAAGCTGCAAAATTGCAGGAAGTGTCAACGAAACCAGTCTACATCCCTAAGATAGA
  CCTGGACCATGATTTGATAAAGGGGGTCTACCAGGGTGCATTGGACCTCAAGAGTGAGCTGAAGCTCTCC
  CTCAAGCAGCTACAGGAAAAGAAGGCTGAGGAGCCCTGCAACTCAGGTAACGTGGGAGACCATGCGCAGT
  CCACATCCAAACCCACACGCCCTGCGAAGAAGACCGCACCGGACAAGAAGACCAAGAAAACCCCTGGTGC
  TGCGGGTGCTGCCGGTGCTGCCGGTGCTGCTGGTGCTGCCGGTCCTACCTCCTACTCTTCCTCATCTAAC
  AAGTTGCCCACCTTTGGAGCTCCTGGACAAGCAACTATCCCTGATGCCACACTCAAACCCAATGCTCCAC
  AACCAAACTCGGCAGCCGTCAAGGCTAAGGTGTTAGAAAACTTCTTAACCATGTCCAGAACAGAGCTTCT
  GGAATATTTCGTTAAAGTCGTCTTCGACTACAATACCGCCCACAACAAAGTGGCTCTGGCAGACAACTAC
  ACCACCGCTTCCGTGGCCGACGGGCTGCAGCACTACCGGTCCCATCCCCAGAGGTTCACCTACTGCTCTC
  AGGTCCTGGGGCTGCACTGCTATAAGAAAGGCATCCACTACTGGGAGGTGGAGCTGCAGAAGAACAACTT
  CTGTGGTGTAGGCGTCTGCTATGGCAGCATGGATCGGCAGGGCCCCGAGAGCCGGCTGGGCCGTAACCCC
  AGCTCATGGTGTGTGGAGTGGTTCAATAACAAGATCTCCGCCTGGCACAACAACATGGAGAAAACGCTGC
  CCTCCACCAAGGCCACGCGAGTCGGCGTGCTACTCAACTGTGACCATGGCTTTGTCATTTTCTTTGCTGT
  CACCGAAAAGGTGTATCTGATGTACAAGTTCAAGGTGGACTTTACTGAAGCTCTGTACCCAGCCTTTTGG
  GTGTTCTCTGCGGGTACCACCCTCTCTATCTGCTCCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206584 representing NM_001009536
  Red=Cloning site Green=Tags(s)
  
  MAELFPLAEELSCSICLEPFKEPVTTPCGHNFCRSCLNETWVVQGPPYRCPQCRTVYQVRPQLHKNTVLC
  AVVEQFLQAEQARTPVDDWIPPTRSAASSSDTQVSCDHCLKEVAVKTCLVCMASFCQEHLRPHIDSPAFQ
  DHPLQPPVRDLLRRKCSRHNRLREMFCPEHGECICHICLVEHKTCSPTILSQASADLENKLRQKLTIMYS
  HINGASKALEDVKSKQQNVQESMKRKMLQLRQEYLEMKAVIDAAETSSTHKLKEEEKRVYGKLDTIYQVL
  LKKKNEMQNLKAEVELILAKGDEFEFLEKAAKLQEVSTKPVYIPKIDLDHDLIKGVYQGALDLKSELKLS
  LKQLQEKKAEEPCNSGNVGDHAQSTSKPTRPAKKTAPDKKTKKTPGAAGAAGAAGAAGAAGPTSYSSSSN
  KLPTFGAPGQATIPDATLKPNAPQPNSAAVKAKVLENFLTMSRTELLEYFVKVVFDYNTAHNKVALADNY
  TTASVADGLQHYRSHPQRFTYCSQVLGLHCYKKGIHYWEVELQKNNFCGVGVCYGSMDRQGPESRLGRNP
  SSWCVEWFNNKISAWHNNMEKTLPSTKATRVGVLLNCDHGFVIFFAVTEKVYLMYKFKVDFTEALYPAFW
  VFSAGTTLSICSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001009536
• ORF Size: 1929 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009536.1, NP_001009536.1
• RefSeq Size: 2447 bp
• RefSeq ORF: 1932 bp
• Locus ID: 494338
• Cytogenetics: 10q26
• MW: 72 kDa
• Gene Summary: may play a role in osteoblast differentiation [RGD, Feb 2006]