Ngly1 (NM_001014136) Rat Untagged Clone

CAT#: RN207395

Ngly1 (untagged ORF) - Rat N-glycanase 1 (Ngly1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Ngly1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN207395 representing NM_001014136
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCGGCCACACTGGGCAGCTCGTCGAGCTCTGCGTCTCCGGCCGTGGCCGAGTTATGCCAGAACA
  CCCCGGAGACCTTTCTGGAGGCCTCCAAGCTGCTGCTCACCTACGCCGACAACATCCTGAGAAACCCCAG
  TGATGAAAAATACAGATCCATCCGCATTGGAAACACAGCCTTTTCTACTAGACTCTTGCCTGTCAGAGGA
  GCTGTTGAGTGTTTATTTGAAATGGGCTTTGAAGAGGGAGAAACACACCTTATCTTTCCTAAAAAAGCTT
  CAGTGGAACAGCTTCAGAAAATCCGTGACCTGATTGCTGTAGAGAGAAGGAGCAGACTGGATGGATCAAG
  TCAGAAGGTGGAGTTCTCTCAGCACCCTGCTGCTGTCAGGCTTCCTGCCGAGCAGCCTGAGGATCCTACA
  GGATTAATGCAGCACTCAGGAAACCAACCTGGGCAGCCGCTGAGCCTACCCTCTGCTCCACTGGTTGTTG
  GTGATTCAACCATCTTTAAAGTTCTTCAGTCCAACATTCAGCACGTGCAGCTCTATGAAAATCCTGTCCT
  CCAGGAGAAAGCCTTGGCTTGTATCCCAGTCAATGAACTCAAAAGAAAATCTCAAGAAAAACTATTCAGA
  GCTAGAAAGTTAGATAAAGGTACTAAAGTAAGTGATGAGGATTTCCTTCTACTGGAGCTCCTACACTGGT
  TTAAAGAGGAGTTTTTCCACTGGGTGAATAACGTTGTGTGCAGCAGATGTGGCAGAGAGACTAGATCTAG
  AGATGAGGCCTTGCCGCCCAATGATGATGAGCTGAAGTGGGGCGCCAAGAATGTGGAGGATCATTACTGT
  GATGCCTGCCAGTTGAGCAACCGCTTCCCAAGATATAACAACCCAGAGAAACTTTTGGAAACAAGATGTG
  GACGCTGTGGTGAATGGGCCAATTGTTTTACGCTATGCTGCCGTGCATTAGGGTTTGAAGCTCGATATGT
  GTGGGATTACACAGACCATGTTTGGACAGAAGTCTATTCTCCATCTCAGCAGCGGTGGCTGCATTGTGAT
  GCATGTGAGGATGTCTGCGACAAACCTCTTCTTTATGAAATAGGATGGGGAAAGAAGCTCTCCTATATCA
  TAGCATTCTCTAAAGATGAGGTGGTTGACGTCACCTGGAGATACTCTTGTAAACATGAAGAGGTGATGTC
  CAGGAGAACCAAGGTCAAAGAAGAGTTACTTAGAGAAACTATCAATGGGCTTAATAAGCAGAGACAGCTA
  TTATTATCAGAAAGCAGAAGAAAAGAACTTCTCCAGAGGATAATTGTGGAGCTTGTTGAATTTATATCTC
  CCAAAACCCCCCGACCTGGAGAACTTGGTGGAAGGGTATCTGGGTCATTGGCTTGGAGAGTGGCCCGAGG
  TGAGACGTGTCTGGAGAGAAAGGAGATTCTGTTCATCCCTTCTGAAAATGAAAAGATTTCTAAGCAATTC
  CACCTCCGTTATGATATTGTGAGAGATCGTTACATCCGTGTCTCAGACAACAATGCAAACATTTCTGGAT
  GGGAGAACGGTGTGTGGAAAATGGAATCCATATTCAGAAAGGTTGAGAAAGACTGGAACATGGTTTATTT
  GGCCCGAAAAGAAGGATCATCTTTTGCTTATATTTCCTGGAAGTTTGAATGTGGGTCAGCTGGTCTAAAG
  GTGGATAACGTTTCCATCCGGACGAGTAGCCAAAGCTTTGAGACTGGATCAGTGAGGTGGAAACTTCGCT
  CTGAGATGGCTCAAGTCAACCTGCTGGGAGGTAGGTCCAGACAGAAATCTGCGTTCCTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001014136
• Insert Size: 1812 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014136.1, NP_001014158.1
• RefSeq Size: 2234 bp
• RefSeq ORF: 1812 bp
• Locus ID: 361014
• Cytogenetics: 15p16