Lig3 (NM_001012011) Rat Untagged Clone

CAT#: RN207667

Lig3 (untagged ORF) - Rat ligase III, DNA, ATP-dependent (Lig3), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Lig3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN207667 representing NM_001012011
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTTGGCCTTTAAGACCCTCTTCCCGAGAAACCTCTGTGCCCTTGGCAGAAAAGAACTGTGCCTGT
  TTTCAGAACAGCATCACTGGCCTGCCATAAGACAGTTCAGCCAGTGGTCGGAAACAAATCTCCTTTGTGG
  GTGCTGCCTCCTCCAGAGAAGAAAGCCTGTCCTATCTTTCCAGAGAGGTCATCTAAGACCACGTGCCACC
  CACCTTATTTCGTGGTCAGGGTCGCATGTGGGACTCTGTACTGGCCCCTGTGAGATGGCCGAACAGCGGT
  TCTGTGTGGACTATGCCAAGCGGGGCACAGCTGGCTGCAAGAAATGCAAGGAGAAGATTGTAAAGGGCGT
  ATGCCGCATTGGCAAAGTGGTACCCAATCCCTTCTCAGAGTCTGGGGGTGATATGAAAGAGTGGTACCAC
  ATTAAGTGCATGTTTGAGAAACTGGAGCGGGCGCGGGCCACCACAAAAAAAATTGAAGACCTCACAGAGC
  TGGAAGGCTGGGAAGAGCTGGAAGATAATGAAAAGGAACAGATAAGCCAGCACATTGCAGACCTGTCTTC
  CAAGACAGCTGCCACACCGAAGAAGAAAGCTACTGTCCAGGCTAAGCTGACAACCACTGGCCAAGTGACA
  TCACCAGTGAAAGGCGCTTCGTTTATTACCAGTACCAATCCCCGGAAATTTTCTGGCTTTTCAGCAGCCA
  AACCGAACAACTCTGAGCAAGACCCCTCAAGCCCTGCTCCTAAGACCAGTCTGTCCGCAAGTAAATGTGA
  CCCTAAGCACAAAGATTGTCTACTACGAGAATTCCGGAAGCTGTGTGCCATGGTAGCTGAAAATCCTAGC
  TATAACACCAAGACCCAGATCATCCACGACTTCTTGCAGAAAGGCTCCACAGGAGATGGCTTCCGAGGCG
  ATGTGTACCTGACAGTGAAGCTGCTGCTGCCTGGTGTCATCAAGAGTGTGTACAACTTGAATGATAAGCA
  GATAGTGAAACTGTTCAGCCGAATTTTTAACTGCAACCCAGATGATATGGCCCGGGACCTAGAACAGGGT
  GACGTGTCCGAGACGATCAGAGTCTTCTTTGAGCAGAGCAAGTCTTTTCCCCCAGCTGCCAAAAGCCTCC
  TCACCATCCAGGAGGTGGATGCATTTCTTCTGCACCTCTCCAAGCTCACCAAAGAGGATGAGCAGCAGCA
  GGCCCTGCAGGACATTGCTTCCAGGTGTACAGCCAATGACCTTAAGTGCATTATCCGGCTGATCAAACAC
  GATCTGAAGATGAACTCGGGTGCAAAACACGTGTTAGATGCCCTTGACCCCAATGCTTATGAAGCCTTCA
  AAGCATCACGCAACCTGCAGGATGTGGTGGAGCGGGTCCTTCACAATGAGCAGGAGGTGGAGAAGGACCC
  AGGCCGGCGTCGGGCTCTGAGTGTCCAGGCCTCATTGATGACACCTGTGCAACCCATGCTGGCTGAGGCC
  TGCAAGTCCATTGAGTATGCAATGAAGAAGTGTCCCAATGGCATGTTCTCTGAGATCAAGTACGATGGTG
  AGCGAGTCCAGGTGCATAAGAAGGGAGACCATTTCAGCTACTTCAGCCGCAGTCTCAAGCCTGTCTTGCC
  TCACAAGGTGGCCCACTTTAAGGACTATATTCCCAAAGCGTTTCCTGGGGGTCAAAGCATGATCTTGGAC
  TCTGAAGTGCTCCTGATTGATAACAACACAGGCAAACCACTGCCCTTTGGGACCCTGGGAGTACACAAGA
  AAGCTGCTTTCCAGGATGCTAATGTCTGCCTGTTTGTTTTTGATTGTATCTACTTTAATGATGTCAGCTT
  GATGGACAGGCCTCTCTGTGAGCGACGGAAGTTTCTTCATGATAACATGGTTGAAATCCGTAACCGGATC
  ATGTTCTCAGAAATGAAGCAAGTCACAAAAGCTTCAGACCTGGCTGACATGATAAACCGGGTGATCCGAG
  AGGGCCTGGAAGGTTTGGTGCTAAAGGATGTGAAGGGTACATATGAGCCTGGAAAACGACACTGGCTGAA
  AGTTAAGAAAGACTATTTGAACGAGGGGGCCATGGCTGATACAGCTGATCTGGTGGTTCTTGGGGCCTTC
  TATGGGCAAGGGAGCAAAGGTGGAATGATGTCCATCTTCCTCATGGGCTGCTATGACCCCGACAGCCAGA
  AGTGGTGCACTGTTACAAAATGTGCTGGAGGCCATGACGATGCCACACTTGCCCGCCTGCAGAAAGAGCT
  AGATATGGTGAAGATTAGCAAGGATCCTAGCAAGATACCCAGCTGGCTGAAGATCAACAAGATCTACTAT
  CCTGACTTTATCGTCCCAGACCCAAAGAAAGCTGCTGTGTGGGAGATCACAGGAGCAGAATTCTCTCGAT
  CTGAGGCGCATACTGCTGATGGGATCTCCATCCGATTTCCTCGATGCACCCGAATCCGGGATGACAAGGA
  CTGGAAATCTGCCACTAACCTCCCCCAACTCAAGGAACTGTACCAGCTGTCCAAAGACAAGGCAGACTTC
  GCTGTGGTGGCTGGAGATGAGGGGAGCTCCACTACTGGAGGCAGCAATGGTGAGAATGAGGGCACTGCTG
  GGTCTACTGTGCCCCGCAAGGGCCCCAAGGGACCCCCCAGTAAGTCCTCTGCCAGTGCCAAGAAGACTGA
  ACAGAAGCTGAATGATCCCAGTAGCAGAGGTGGAGAGAAGCTGGCTGTGAAGTCTTCTCCTGTCAAAGTG
  GGGATGAAGAGGAAAGCTGCTGATGAGACCCCAGGCCTGACAAAGAGGCGGCGGGCCAGCCGGCAAAGAG
  GAAGGAGAGCTATGCGAACAGGCAGGAGATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001012011
• Insert Size: 2832 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012011.1, NP_001012011.1
• RefSeq Size: 3874 bp
• RefSeq ORF: 2832 bp
• Locus ID: 303369
• Cytogenetics: 10q26