Nphs1 (NM_022628) Rat Untagged Clone

CAT#: RN208911

Nphs1 (untagged ORF) - Rat nephrosis 1 homolog, nephrin (human) (Nphs1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Nphs1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN208911 representing NM_022628
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGCTAAGAGAGTCACTGTCAGAGGTGCCCGGACAAGCCCAATACACAGAACGTCCAGTTTGACTC
  CCCTGCTGCTCATGGGAATGCTGACCTCAGGCCTGGCCGAGTCGCCAGTCCCCACCTCAGCACCTCAAGG
  CTTCTGGGCTCTGTCTGAAAACCTGACTGCGGTGGAAGGGACAACAGTTAAGCTATGGTGCGGTGTCAGG
  GCCCCTGGCAGTGTGGTGCAGTGGGCTAAGGATGGGCTGCTTCTGGGTCCAAACCCGAAGATGCCAGGCT
  TCCCGAGGTACAGCCTGGAAGGAGATCGTGCTAAAGGCGAGTTCCACCTGCTTATTGAAGCCTGTGACCT
  CAGTGATGACGCAGAGTATGAATGCCAAGTCGGCCGCTCAGAGTTGGGTCCCGAGCTTGTGTCTCCTAAA
  GTAATCCTCTCCATTCTAGTTTCCCCCAAGGTGCTTCTGTTGACCCCCGAGGCAGGAAGCACAGTGACCT
  GGGTAGCTGGGCAGGAGTATGTGGTCACCTGTGTGTCTGGAGATGCAAAACCAGCACCTGACATCACCTT
  CATCCAGAGTGGACGAACTATATTGGACGTCTCCTCCAATGTGAATGAGGGATCAGAGGAGAAACTCTGC
  ATCACAGAGGCCGAAGCCAGGGTGATACCCCAGAGCTCGGATAACGGGCAGTTACTGGTCTGTGAGGGTT
  CCAACCCAGCTTTGGACACTCCCATAAAGGCTTCATTCACCATGAATATTCTGTTTCCCCCAGGACCTCC
  TGTCATTGATTGGCCAGGCCTGAATGAGGGGCATGTGAGGGCAGGGGAGAACCTGGAGCTGCCCTGCACA
  GCCAGAGGTGGCAATCCACCTGCTACCCTGCAGTGGCTGAAGAACGGTAAACCAGTGTCCACAGCCTGGG
  GCACCGAGCATGCCCAGGCAGTGGCCCACAGCGTGCTGGTGATGACTGTACGACCTGAAGACCATGGAGC
  TCGGCTCAGCTGTCAGTCCTACAACAGCGTGTCTGCAGGGACCCAGGAGAGAAGCATCACACTACAGGTC
  ACCTTTCCCCCAAGCGCCATTACCATCCTGGGATCTGTATCACAATCGGAGAACAAGAACGTGACCCTTT
  GCTGCCTGACCAAGTCCAGTCGCCCACGGGTCCTGCTGCGATGGTGGTTGGGTGGACGGCAGCTGCTGCC
  CACAGATGAGACAGTCATGGATGGCCTGCATGGTGGCCACATCTCCATGTCCAATCTCACATTCTTGGTG
  CGGAGAGAAGACAATGGCCTGCCCCTCACCTGTGAAGCCTTCAGTGACGCCTTCAGCAAGGAGACCTTCA
  AGAAGTCACTCACCTTGAATGTGAAATACCCTGCCCAGAAGCTGTGGATTGAGGGGCCCCCAGAGGGACA
  GTACATCCGGACTGGGACTCGGGTGAGGCTGGTATGCTTGGCCATCGGAGGCAACCCAGACCCCTCCCTC
  ATCTGGTTTAAGGATTCACGTCCGGTGAGCGAGCCCCGGCAGCCCCAGGAGCCCCGGCGTGTGCAGCTGG
  GCAGTGTGGAGAAGTCCGGGAGCACTTTCTCCCGCGAGCTGGTGTTGATCATAGGTCCGCCGGACAACCG
  AGCCAAGTTCTCCTGCAAGGCGGGTCAGCTCAGTGCGTCTACGCAGCTGGTGGTGCAGTTCCCCCCAACC
  AACCTGACCATCCTGGCCAACTCGTCCGCGCTGCGCCCAGGCGACGCCTTGAACTTGACCTGCGTCAGCA
  TCAGCAGCAACCCCCCAGTCAACTTGTCTTGGGACAAGGAAGGAGAGAGGCTGGAAGATGTGGCTGCAAA
  ACCCCAGAGTGCACCGTTCAAAGGCTCCGCTGCATCCAGGAGTGTTTTTCTCAGAGTGTCATCCCGAGAC
  CACGGTCAACGGGTCACCTGCCGGGCCCACAGCGAGGCACTCCGTGAAACCGTGAGCTCCTTCTACCGCT
  TCAATGTGCTGTATCCTCCAGAATTCCTGGGGGAGCAAGTCCGGGCAGTGACCGTGGTGGAGCAGGGCCA
  GGTGCTGCTGCCGGTGTCGGTGTCCGCTAACCCCGCCCCCGAGGCCTTCAACTGGACCTTCCGAGGCTAC
  CGCCTCAGCCCAGCTGGGGGTCCCCGGCACCGTATCCTGTCTGGAGGGGCTCTGCAGCTGTGGAATGTGA
  CCCGAGCTGACGATGGCTTTTATCAGCTGCACTGCCAGAACTCAGAGGGCACCGCTGAGGCGCTGTTGAA
  GCTGGACGTGCATTATGCTCCCACCATCCGTGCCCTCCGGGACCCTACTGAGGTGAATGTTGGGGGTTCT
  GTGGACATAGTCTGCACCGTTGACGCCAATCCCATCCTCCCAGAGATGTTCAGCTGGGAGAGACTGGGAG
  AAGAAGAGGAGGATCTGAACCTGGACGACATGGAGAAAGTTTCCAAGGGATCCACGGGGCGTCTGCGGAT
  TCGCCAAGCCAAGCTATCCCAGGCTGGTGCCTACCAGTGCATCGTGGACAATGGGGTGGCTCCTGCAGCC
  AGAGGACTGGTTCGTCTTGTCGTCCGATTTGCTCCCCAGGTGGATCAGCCTACTCCCCTAACAAAAGTGG
  CTGCCGCTGGGGACAGCACCAGCTCAGCCACACTGCACTGCCGTGCCCGGGGTGTCCCCAACATCGACTT
  CACTTGGACCAAAAACGGGGTCCCTCTGGATCTCCAAGACCCCAGGTACACAGAGCACAGGTACCACCAG
  GGTGTTGTCCACAGCAGCCTCTTGACCATCGCTAATGTGTCTGCGGCCCAGGACTATGCCCTCTTCAAAT
  GCACGGCCACCAATGCCCTTGGCTCTGACCACACCAACATCCAGCTCGTCAGCATCAGCCGCCCTGACCC
  TCCACTGGGACTGAAGGTTGTCAGCATAAGCCCTCACTCGGTGGGGCTGGAGTGGAAGCCTGGCTTTGAT
  GGGGGTCTGCCTCAGAGGTTCCAAATCAGGTACGAGGCCCTCGAGACCCCAGGATTCCTCCACGTGGATG
  TCCTACCTACACAGGCCACTACCTTCACGCTGACTGGGCTGAAGCCTTCTACACGATATAGGATCTGGCT
  GTTGGCCAGCAATGCCCTGGGGGACAGTGGATTGACGGACAAGGGGATCCAGGTCTCCGTCACTACCCCA
  GGCCCCGACCAGGCTCCTGAAGACACAGACCACCAGCTGCCCACAGAGCTGCCTCCAGGACCCCCAAGGC
  TGCCCCTGCTGCCTGTGCTCTTTGCAGTTGGTGGTCTTCTGCTGCTCTCCAATGCCTCCTGTGTTGGGGG
  TCTCCTCTGGCGGAGAAGACTGAGGCGCCTTGCTGAGGAGATCTCAGAGAAGACAGAGGCAGGGTCGGAG
  GACAGGATCAGGAATGAATATGAGGAGAGTCAGTGGACTGGGGACCGGGACACGAGAAGCTCCACGGTTA
  GCACAGCAGAAGTGGACCCAAATTACTACTCCATGAGGGACTTCAGCCCCCAGCTTCCCCCAACACTGGA
  GGAGGTGCTGTATCACCAAGGTGCTGAAGGCGAGGACATGGCCTTCCCCGGACACCTGCATGATGAAGTG
  GAGAGAGCCTATGGCCCGCCTGGGGCCTGGGGACCCCTCTATGATGAAGTACGAATGGACCCCTATGACC
  TTCGCTGGCCTGAGGTCCAGTGTGAGGATCCGAGGGGAATCTACGACCAGGTGGCAGCAGACATGGATGC
  TGTGGAAGCTAGCTCTCTGCCGTTTGAGCTGAGGGGACATCTGGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_022628
• ORF Size: 3759 bp
• Insert Size: 3759
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_022628.1, NP_072150.1
• RefSeq Size: 5820
• RefSeq ORF: 3759
• Locus ID: 64563
• Gene Summary: renal protein localized specifically to glomerular capillary walls; may contribute to the development of proteinuria in renal disease [RGD, Feb 2006]