Tspan2 (NM_022589) Rat Untagged Clone

CAT#: RN208985

Tspan2 (untagged ORF) - Rat tetraspanin 2 (Tspan2), (10 ug)

Product Images

Circular map for RN208985

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Tspan2
• Synonyms: Tspan-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >NM_022589 ORF sequence, RN208985 may differ due to SNPs.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGGCGTTTTCGCGGGGGCCTGCGGTGCATCAAGTATTTGCTGCTCGGCTTCAACCTGCTGTTCTGG
  CTAGCCGGATCGGCCGTTATTGCATTTGGACTATGGTTTCGATTTGGAGGTACCATAAAGGATTTATCA
  TCAGAGGAGAAGTCCCCAGAGTATTTCTATGTGGGGCTGTATGTGCTGGTTGGAGCTGGAGCCCTAATG
  ATGGCTGTGGGTTTCTTTGGGTGCTGTGGAGCCATGCGTGAGTCACAGTGTGTGCTCGGATCTTTTTTT
  ACCTGTCTGCTGGTGATATTCGCTGCTGAAGTAACCACTGGAGTGTTTGCATTTATAGGCAAGGATGTA
  GCTATACGACACGTACAGAGCATGTATGAGGAGGCCTACAGCGATTACGTTAGGGACAGGGGAAGGGGA
  AATGGAACTCTCATTACCTTCCACTCAGCATTTCAGTGCTGTGGAAAAGAAAGCTCTGAACAAGTCCAG
  CCCACGTGTCCAAAGGAGCTTCCAGGCCACAAGAATTGCATCGACAAAATTGAAACCATAATCAGTGTT
  AAGCTCCAGCTCATTGGAATTGTTGGTATTGGGATTGCGGGTCTCACGATCTTTGGCATGATATTCAGC
  ATGGTGCTCTGCTGTGCAATACGGAACTCACGCGATGTGATTTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  MGRFRGGLRCIKYLLLGFNLLFWLAGSAVIAFGLWFRFGGTIKDLSSEEKSPEYFYVGLYVLVGAGALM
  MAVGFFGCCGAMRESQCVLGSFFTCLLVIFAAEVTTGVFAFIGKDVAIRHVQSMYEEAYSDYVRDRGRG
  NGTLITFHSAFQCCGKESSEQVQPTCPKELPGHKNCIDKIETIISVKLQLIGIVGIGIAGLTIFGMIFS
  MVLCCAIRNSRDVI*
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Fully Sequenced ORF

  >RN208985 representing NM_022589
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCGTTTTCGCGGGGGCCTGCGGTGCATCAAGTATTTGCTGCTCGGCTTCAACCTGCTGTTCTGGC
  TAGCCGGATCGGCCGTTATTGCATTTGGACTATGGTTTCGATTTGGAGGTACCATAAAGGATTTATCATC
  AGAGGAGAAGTCCCCAGAGTATTTCTATGTGGGGCTGTATGTGCTGGTTGGAGCTGGAGCCCTAATGATG
  GCTGTGGGTTTCTTTGGGTGCTGTGGAGCCATGCGTGAGTCACAGTGTGTGCTCGGATCTTTTTTTACCT
  GTCTGCTGGTGATATTCGCTGCTGAAGTAACCACTGGAGTGTTTGCATTTATAGGCAAGGATGTAGCTAT
  ACGACACGTACAGAGCATGTATGAGGAGGCCTACAGCGATTACGTTAGGGACAGGGGAAGGGGAAATGGA
  ACTCTCATTACCTTCCACTCAGCATTTCAGTGCTGTGGAAAAGAAAGCTCTGAACAAGTCCAGCCCACGT
  GTCCAAAGGAGCTTCCAGGCCACAAGAATTGCATCGACAAAATTGAAACCATAATCAGTGTTAAGCTCCA
  GCTCATTGGAATTGTTGGTATTGGGATTGCGGGTCTCACGATCTTTGGCATGATATTCAGCATGGTGCTC
  TGCTGTGCAATACGGAACTCACGCGATGTGATTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_022589
• Insert Size: 666 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022589.2
• RefSeq Size: 3833 bp
• RefSeq ORF: 666 bp
• Locus ID: 64521
• UniProt ID: Q9JJW1
• Cytogenetics: 2q34
• MW: 24.2 kDa
• Gene Summary: may mediate signalling in oligodendrocytes in the early stages of their terminal differentiation into myelin-forming glia; may function in stabilizing the mature sheath [RGD, Feb 2006]