Nnt (NM_001013157) Rat Untagged Clone

CAT#: RN209345

Nnt (untagged ORF) - Rat nicotinamide nucleotide transhydrogenase (Nnt), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Nnt
• Synonyms: MGC109126
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN209345 representing NM_001013157
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACATCTACTGAAAACGGTGGTGACTGGCTGTTTATGTCCTTCTGTTAGCAATTTGGGGTCCTGTA
  AGGTTCTACCTGGGAAGAAAGACTTTTTACGAACATTTCGTACCCACCAGGCACTGTGGTGTAAATCCCC
  GGCAAAACCAGGAATTCCATATAAGCAGTTGACAGTTGGGGTCCCCAAGGAGATTTTCCAAAATGAGAAG
  CGAGTTGCCTTGTCTCCTGCTGGTGTCCAGGCCCTGGTCAAGCAGGGTTTTAACGTTGTTGTGGAATCAG
  GAGCAGGCGAAGCTTCCAAATTCCCAGATGATCTCTACAGAGCAGCAGGGGCCCAGATCCAAGGAACGAA
  GGAAGTTCTGGCTTCTGATCTGGTGGTCAAAGTGAGGGCCCCCATGGTGAACCCGACGTTAGGTGCTCAT
  GAAGCTGACTTTTTAAAGCCCTCAGGAACTCTAATTAGCTTCATTTACCCAGCCCAAAATCCAGACTTGC
  TAAATAAACTCTCCGAAAGAAAAACTACAGTTTTGGCGATGGACCAGGTTCCGAGGGTGACAATAGCTCA
  GGGCTATGATGCTCTCAGCTCCATGGCCAACATTTCTGGTTATAAGGCTGTTGTTTTAGCAGCCAATCAT
  TTTGGAAGGTTTTTCACTGGTCAGATCACAGCTGCTGGAAAAGTCCCCCCAGCGAAGATCCTGATAGTTG
  GTGGTGGCGTTGCCGGGCTCGCTTCTGCAGGGGCAGCAAAGTCAATGGGCGCAGTTGTCCGAGGATTTGA
  TACAAGAGCTGCCGCCTTGGAACAGTTCAAATCCCTTGGTGCTGAACCCTTGGAGGTGGACTTGAAGGAG
  TCTGGTGAGGGCCAAGGAGGATACGCGAAGGAGATGTCCAAAGAGTTTATTGAGGCGGAAATGAAGCTCT
  TTGCTCAGCAATGCAAGGAAGTGGACATCCTTATCAGCACAGCTCTGATTCCAGGTAAGAAAGCTCCTGT
  TCTATTTAGTAAAGAAATGATCGAGTCCATGAAGGAAGGTTCAGTAGTTGTGGATTTAGCTGCTGAGGCC
  GGCGGAAACTTTGAAACGACTAAACCGGGAGAACTTTACGTTCATAAGGGAATCACTCATATTGGCTACA
  CAGACCTCCCCAGCCGCATGGCCACTCAGGCTAGCACTCTGTACTCCAACAACATCACCAAGCTCCTGAA
  GGCCATCAGCCCCGACAAGGACAATTTTCACTTTGAAGTGAAGGATGACTTTGACTTTGGTACAATGAGT
  CACGTCATTCGAGGAACCGTGGTGATGAAGGATGGCAATGTGATTTTCCCAGCTCCCACACCAAAAAATA
  TTCCCAAAGAAGCCCCAGCCAAACAGAAGACCGTGGCTGAGCTGGAAGCAGAGAAGGCAGGCACTGTCTC
  AATGTACACGAAGACACTGAGGACAGCATCCGTGTATTCAGCAGGTCTCACCGGGATGCTAGGTTTGGGC
  ATCGTGGCCCCCAATCTAGCCTTTTCTCAGATGGTGACCACCTTTGGCCTGGCGGGCATTATTGGTTACC
  ACACTGTCTGGGGAGTGACCCCTGCTCTCCACTCACCACTGATGTCTGTGACCAATGCTATCTCAGGTTT
  GACTGCAGTTGGTGGGCTGGCGTTGATGGGAGGGCATTTTTATCCCTCCACAACTTCCCAGAGTCTTGCT
  GCTCTTGCTACATTCATATCGTCAGTCAACATTGCAGGTGGTTTTCTGGTGACTCAGAGAATGTTGGACA
  TGTTCAAGCGACCCACGGACCCTCCAGAATACAATTACCTGTATCTGCTCCCTGGCGGCACCTTCGTGGG
  GGGATATTTAGCAGCTCTCTACGGTGGTTATAACATCGAAGAAATCATGTACCTGGGCTCAGGTTTGTGC
  TGTGTTGGGGCCCTGGGCGGCCTTTCCACTCAGGGAACAGCTCGACTCGGCAATGCCTTGGGCATGATTG
  GGGTTGCAGGAGGCCTGGCAGCTACCCTTGGAGGCCTGAAGCCGGACCCACAGCTGTTAGCTCAGATGTC
  CGGAGCAATGGCGATGGGCGGCACCATCGGACTGGCGATTGCAAAGCGGATCCAGATCTCCGACCTGCCT
  CAACTGGTTGCTGCTTTCCACAGTTTAGTGGGTCTGGCGGCAGTGCTCACATGCATGGCCGAGTACATCG
  TGGAGTATCCACATTTCGCAATGGATGCCACTTCAAATTTCACCAAGATTGTGGCCTACATTGGCACTTA
  CATCGGCGGAGTCACCTTTAGTGGGTCTCTGGTTGCCTACGGAAAATTACAGGGTATTCTGAAGTCAGCC
  CCTCTGCTGCTCCCTGGAAGGCACGCGCTCAATGCAGGCTTGCTGGCTGCTAGCGTGGGGGGAATAATCC
  CATTTATGGCTGACCCTAGTTTTACCACAGGCATCATGTGTCTGGGCTCTGTGTCCGCACTCTCTACTCT
  GATGGGTGTGACCTTGACAGCTGCCATTGGCGGTGCTGACATGCCTGTTGTTATCACTGTGCTAAACAGC
  TACTCCGGCTGGGCTCTGTGTGCGGAGGGCTTCCTGCTCAACAACAACCTGTTGACCATTGTGGGAGCAC
  TCATCGGCTCCTCGGGCGCCATCTTGTCGTACATCATGTGTGTGGCAATGAACCGTTCCCTGGCAAATGT
  AATTCTCGGAGGCTATGGTACGACTTCAACAGCTGGTGGAAAACCCATGGAAATTTCCGGCACACATACA
  GAAATCAACCTGGACAATGCAGTCGAGATGATCCGGGAAGCTAACAGCATTGTTATTACTCCAGGATATG
  GGCTCTGTGCAGCTAAAGCTCAGTACCCCATTGCCGATTTGGTGAAGATGCTTACCGAACAAGGCAAGAA
  AGTCAGGTTTGGAATCCACCCAGTTGCGGGCCGAATGCCTGGTCAGCTTAATGTGCTCCTGGCCGAGGCA
  GGAGTGCCCTATGATATTGTGCTAGAAATGGATGAGATCAACAGTGATTTCCCAGATACCGATCTGGTTC
  TTGTCATTGGAGCTAATGACACCGTGAATTCAGCGGCTCAGGAAGACCCCAATTCTATTATTGCAGGCAT
  GCCAGTCCTCGAGGTCTGGAAGTCCAAACAGGTCATTGTCATGAAGAGGTCCCTTGGTGTTGGCTACGCT
  GCAGTGGACAATCCAATCTTCTACAAACCTAACACAGCCATGCTTCTGGGGGACGCCAAGAAGACATGTG
  ATGCTCTGCAGGCCAAAGTTCGAGAATCCTATCAGAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001013157
• Insert Size: 3261 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013157.1, NP_001013175.1
• RefSeq Size: 4149 bp
• RefSeq ORF: 3261 bp
• Locus ID: 310378
• Cytogenetics: 2q15