Il6st (NM_001008725) Rat Untagged Clone

CAT#: RN210402

Il6st (untagged ORF) - Rat interleukin 6 signal transducer (Il6st), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Il6st
• Synonyms: Ac1055; Gp130; Il-6rb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN210402 representing NM_001008725
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGCACTAAGGATCTGGCTAATGCAAGCTTTGCTTATTTTCCTCACTACTGAGTCTATAGGTCAAC
  TTGTGGAACCATGTGGTTATATCTACCCTGAGTTTCCAGTTGTCCAGCGTGGCTCTAACTTCACTGCCAC
  TTGTGTGCTAAAGGAGAAGTGTCTGCAGGTGTACTCCGTGAATGCCACTTACATCGTGTGGAAGACAAAC
  CATGTTGCCGTTCCTAAGGAGCAGGTCACAGTCATCAACAGAACGGCATCCAGTGTCACATTCACAGACG
  TGGTCTTTCAGAACGTCCAGCTCACCTGCAATATCCTGTCATTTGGACAGATCGAGCAGAATGTGTATGG
  AATCACCATACTTTCAGGCTATCCTCCAGATATACCTACAAATTTGAGTTGCATTGTGAATGAGGGGAAG
  AACATGCTGTGTCAGTGGGACCCAGGAAGGGAGACATACCTTGAAACAAACTACACTTTGAAGTCAGAGT
  GGGCAACAGAGAAGTTTCCTGATTGTCGAACAAAGCATGGCACGAGCTCCTGCATGATGGGCTATACCCC
  CATCTATTTTGTCAACATCGAGGTCTGGGTGGAGGCCGAGAATGCCCTTGGGAATGTCTCCTCAGAGCCT
  ATCAATTTTGACCCCGTGGATAAAGTGAAACCCAGCCCACCTCATAATTTGTCAGTGACCAACTCAGAAG
  AATTATCCAGTATATTAAAACTAGCATGGGTCAATTCAGGTTTGGACAGTATTTTAAGGCTGAAGTCGGA
  CATCCAATATAGGACCAAAGATGCCTCAACTTGGATTCAGGTCCCTCTTGAAGATACAGTGTCTCCCCGG
  ACTTCGTTCACTGTTCAGGATCTCAAGCCTTTTACAGAATATGTGTTTAGGATTCGGTCCATTAAGGAGA
  ATGGGAAGGGCTACTGGAGTGACTGGAGTGAGGAGGCGAGCGGGACCACATATGAAGACAGACCATCCAA
  AGCGCCAAGTTTCTGGTATAAGGTAAATGCAAACCATCCACAGGAGTATAGGTCTGCACGGCTCATATGG
  AAGACATTGCCCCTTTCTGAAGCCAATGGGAAGATCTTGGATTATGAAGTGGTTCTTACACAGTCCAAGT
  CAGTTTCGCAAACTTACACAGTTAACGGCACAGAGTTGATAGTAAACCTCACCAATAACCGCTATGTAGC
  GTCTCTAGCAGCAAGAAATGTGGTCGGCAAGTCCCCTGCAACCGTCCTCACCATCCCCGGCTCCCACTTC
  AAAGCTTCTCACCCCGTAGTGGATCTTAAAGCATTTCCAAAAGATAACCTGCTCTGGGTAGAATGGACAC
  CGCCATCTAAACCTGTGAACAAATACATACTAGAGTGGTGTGTGTTGTCAGAGAACTCACCCTGCATCCC
  AGACTGGCAGCAAGAAGATGGCACTGTGAATCGGACCCACTTAAGAGGAAGCCTACTGGAGAGCAAGTGC
  TATCTGATCACAGTAACCCCAGTGTTTCCCGGTGGGCCTGGAAGCCCTGAGTCCATGAAGGCGTACCTCA
  AACAAGCAGCTCCTTCTAAAGGACCGACTGTTCGGACAAAGAAAGTGGGGAAAAATGAAGCTGTCTTAGA
  GTGGGACCATCTTCCTGTTGATGTCCAGAACGGATTCATTAGAAACTACTCCATATCTTATAGAACCAGT
  GTTGGAAAAGAAATGGTTGTGCGTGTGGATTCTTCTCACACAGAATACACACTGTCCTCTTTGAGTAGCG
  ATACACTGTACATGGTCCACATGGCAGCATACACAGAAGAAGGTGGGAAGGATGGGCCGGAATTCACTTT
  TACAACACTAAAGTTTGCTCAAGGAGAAATCGAAGCCATAGTCGTGCCTGTGTGCTTAGCTTTCCTCCTG
  ACAACGCTGCTGGGAGTCTTGTTCTGCTTTAATAAACGAGACCTAATTAAAAAACACATCTGGCCGAATG
  TCCCAGATCCTTCAAAGAGTCATATTGCCCAGTGGTCACCTCACACCCCCCCAAGGCACAATTTTAACTC
  CAAAGATCAGATGTACTCAGATGCCAATTTCACTGACGTAAGCGTTGTGGAAATAGAAGCAAACAACAAA
  AAGCCTTGTCCAGATGACCTGAAATCCTTGGACCTGTTCAAGAAGGAGAAAATAAGTACAGAAGGGCACA
  GCAGTGGCATTGGGGGGTCCTCGTGCATGTCCTCTTCTAGGCCCAGCATCTCCAGCAGTGAGGAGAACGA
  GTCTGCTCAGAGCACCGCAAGCACGGTGCAGTACTCCACCGTGGTGCACAGCGGCTACAGGCACCAGGTG
  CCGTCAGTGCAAGTGTTCTCAAGGTCTGAGTCCACCCAGCCCCTGCTAGACTCCGAGGAGCGGCCAGAGG
  ACCTGCAGCTGGTGGATAGTGTGGACAGCGGTGACGAGATCTTACCCAGGCAACAGTATTTCAAACAAAG
  CTGCAGTCAGCCTGGAGCCAGTCCAGATGTTTCACACTTCGAAAGGTCAAGCCAGGTTCCGTCAGGCAGT
  GAGGAGGATTTTGTCAGACTGAAACAGCAGCAGGTTTCAGATCACATTTCAGAGCCCTATGGCTCCGAGC
  AACGGAGGCTGTTTCAGGAAGGTTCTGTGGCAGATGCTCTTGGCACAGGGACTGATGGACAGATAGAGAG
  ATTCGAATCTGTTGGAATGGAGACAGCGATGGATGAAGACATTTCCAAAAGTTACTTGCCACAGACTGTA
  AGACAAGGTGGCTACATGCCACAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001008725
• Insert Size: 2757 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008725.3, NP_001008725.2
• RefSeq Size: 7454 bp
• RefSeq ORF: 2757 bp
• Locus ID: 25205
• Cytogenetics: 2q14
• Gene Summary: IL-6 signal transducing molecule [RGD, Feb 2006]