Uba1 (NM_001014080) Rat Untagged Clone

CAT#: RN211933

Uba1 (untagged ORF) - Rat ubiquitin-like modifier activating enzyme 1 (Uba1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Uba1
• Synonyms: Ube1x
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN211933 representing NM_001014080
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAGCTCGCCGCTGTCCAAGAAACGTCGCGTGTCCGGGCCTGATCCAAAGCCAGGTTCTAACTGCT
  CCTCTGCACAGTCAGTGCTGTCCGAAGTGTCTTCAGTGCCAACCAACGGAATGGCGAAGAACGGCAGTGA
  AGCAGACATAGACGAGAGCCTTTACTCCCGGCAGCTGTACGTTTTGGGCCATGAGGCAATGAAAATGCTC
  CAGACATCCAGTGTCCTCGTCTCAGGCTTGCGGGGCTTAGGTGTCGAGATTGCTAAGAACATCATCCTTG
  GTGGGGTCAAGGCTGTCACCCTACATGACCAAGGCACTACACAGTGGGCTGATCTCTCCTCCCAGTTTTA
  CCTTCGGGAGGAGGACATTGGTAAAAATCGAGCTGAGGTATCCCAGCCCCGACTTGCTGAACTCAACAGC
  TATGTACCTGTTACTGCCTACACTGGGCCTCTTGTTGAAGACTTCCTTAGTGGTTTCCAGGTGGTGGTCC
  TTACCAACAGCCCCCTGGAAGAACAGCTTCGAGTGGGTGAGTTCTGTCATAGCCGTGGCATCAAGCTAGT
  GGTGGCAGATACAAGAGGCCTATTTGGGCAACTTTTCTGTGATTTTGGAGAGGAAATGGTTCTCACAGAT
  TCCAATGGGGAGCAGCCACTCAGTGCTATGGTTTCAATGGTCACCAAGGACAACCCCGGTGTGGTTACCT
  GCCTTGATGAGGCCCGACATGGCTTTGAGACTGGTGACTTCGTCTCATTCTCAGAAGTACAGGGCATGGT
  CCAACTCAATGGATGTCAGCCCATAGAGATCAAAGTGCTGGGTCCTTATACTTTTAGTATCTGTGACACC
  TCCAACTTCTCTGACTACATCCGTGGAGGCATCGTCAGCCAGGTCAAAGTACCTAAAAAGATTAGCTTTA
  AATCCTTGCCAGCATCACTGGCAGAGCCTGACTTTGTGATGACTGACTTTGCCAAGTATTCTCGCCCTGC
  CCAACTGCACATTGGCTTTCAAGCTCTGCACCAATTCTGTGCTCAGCACAACCGACCACCTCGACCACGA
  AATGAGGAAGATGCAACAGAACTGGTGACCCTAGCTCAGGCTGTGAATGCTCGGTCCCCACCTGCAGTAC
  AACAGGACAACGTGGATGAAGACCTTATTCGGAAGCTAGCTTATGTTGCTGCTGGGGACCTGGCACCTAT
  AAATGCTTTCATTGGGGGCCTCGCTGCCCAGGAAGTCATGAAGGCCTGTTCTGGAAAGTTTATGCCCATC
  ATGCAGTGGTTGTACTTTGATGCTCTTGAATGTCTCCCAGAGGACAAAGAGGCTCTGACAGAGGACAAGT
  GCCTCCCACGTCAGAACCGTTATGATGGGCAGGTAGCTGTATTTGGCTCAGACCTTCAAGAGAAGCTGGG
  CAAGCAAAAGTACTTCCTGGTGGGTGCAGGGGCCATTGGCTGTGAATTGCTCAAGAACTTTGCCATGATT
  GGGTTAGGCTGTGGAGAGGGTGGAGAGGTCGTGGTCACAGACATGGACACCATTGAGAAATCGAATCTGA
  ACCGACAGTTTCTTTTCCGGCCCTGGGATGTCACGAAGTTAAAGTCTGACACGGCCGCTGCAGCTGTGCG
  ACAGATGAATCCTTACATCCAGGTGACAAGCCACCAGAACCGTGTAGGCCCTGACACGGAGCGCATCTAT
  GATGATGATTTTTTCCAAAATTTGGATGGTGTGGCCAATGCACTGGACAATGTAGATGCCCGGATGTACA
  TGGATCGCCGCTGTGTGTACTATCGGAAGCCACTGCTAGAGTCTGGCACACTGGGCACAAAGGGCAATGT
  GCAGGTGGTGATCCCCTTCCTGACAGAATCATACAGCTCTAGCCAGGACCCACCAGAGAAATCCATCCCC
  ATTTGTACCCTGAAAAACTTTCCCAATGCCATCGAACACACTCTACAGTGGGCCCGGGATGAATTTGAAG
  GCCTTTTCAAGCAGCCAGCAGAAAATGTTAATCAGTACCTCACAGACTCCAAATTTGTGGAGCGGACATT
  GCGGCTGGCGGGTACCCAGCCATTGGAGGTGCTGGAGGCTGTGCAGCGCAGCCTGGTGCTGCAGCGACCA
  CAGACTTGGGGCGACTGTGTGACCTGGGCCTGCCACCACTGGCACACCCAGTACTGTAACAACATACGGC
  AGCTGCTGCACAACTTTCCTCCCGACCAGCTCACCAGCTCAGGGGCCCCTTTCTGGTCTGGACCCAAACG
  ATGTCCACACCCACTTACTTTTGATGTTAACAATACATTGCATCTGGATTATGTGATGGCTGCTGCCAAC
  CTTTTTGCCCAGACCTATGGGTTGACTGGCTCCCAAGACCGAGCTGCTGTGGCCTCACTCCTACAGTCAG
  TACAAGTTCCAGAGTTCACCCCCAAGTCTGGTGTCAAGATCCATGTTTCTGACCAGGAGTTGCAGAGCGC
  CAATGCCTCTGTTGATGACAGCCGTCTCGAGGAGCTCAAAGCCACACTGCCGAGCCCAGACAAGTTACCT
  GGATTTAAGATGTACCCCATTGATTTTGAAAAGGACGATGACAGCAATTTCCACATGGATTTCATTGTGG
  CTGCATCTAATCTTCGGGCCGAAAACTATGATATTTCCCCTGCAGACCGGCACAAGAGCAAGCTGATCGC
  AGGGAAGATCATCCCAGCTATCGCCACAACCACAGCTGCTGTGGTTGGCCTTGTGTGTCTGGAACTCTAC
  AAGGTAGTTCAGGGGCACCAACAACTTGATTCCTACAAAAATGGTTTCCTGAATTTGGCCCTGCCCTTCT
  TTGGGTTTTCGGAACCTCTTGCTGCGCCTCGTCACCAGTACTATAATCAAGAGTGGACATTGTGGGATCG
  CTTTGAAGTACAAGGGCTACAGCCTAATGGTGAGGAGATGACTCTCAAGCAGTTCCTTGATTACTTTAAG
  ACGGAGCACAAATTAGAGATCACCATGCTGTCCCAGGGTGTGTCCATGCTCTATTCTTTCTTCATGCCAG
  CTGCTAAGCTCAAGGAACGGTTGGATCAGCCGATGACAGAGATTGTGAGCCGAGTGTCAAAGAGAAAGCT
  GGGCCGCCATGTGCGTGCACTGGTGCTTGAGCTGTGCTGCAACGATGAAAGTGGCGAGGACGTCGAGGTC
  CCCTATGTACGATATACAATTCGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001014080
• Insert Size: 3177 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014080.1, NP_001014102.1
• RefSeq Size: 3618 bp
• RefSeq ORF: 3177 bp
• Locus ID: 314432
• Cytogenetics: Xq11