Egln2 (NM_001004083) Rat Untagged Clone

CAT#: RN212035

Egln2 (untagged ORF) - Rat EGL nine homolog 2 (C. elegans) (Egln2), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Egln2
• Synonyms: HIF-PH1; HPH-1; HPH-3; PHD-1; PHD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN212035 representing NM_001004083
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGCCCGTGCCAGCCGCAGGCCCTGAATCAAGCTCTCCCTCAGTTGCCAGGGTCTGTGTCAGAGT
  CCTTGGAGCCTAGCCGAGCCAGGATGGGGGTGGAGAGTTACCTGCCCTGTCCTCTGCTCCCCTCCTACCA
  CCGTTCAGGAGCATCTGGGGAGGCCTCGGCTGGCAACGGGACCCCCAGAACCACAGCCACTGCTACTACT
  ACCACTGCCAGTCCCCTGCGGGAGGGCTTTGGTGGGCAGGATGGTGGTGAGCTGTGGCCACTGCAGAGTG
  AAGGTGCTGCTGCGTTGGTTACCAAGGAGTGCCAGCGACTGGCGGCCCAGGGTGCCCGACCTGAGGCCCC
  CAAACGGAAGTGGGCCAAGGATGGTGGGGATGCCCCTTCACCCAGCAAGCGACCGTGGGCCAGGCAAGAG
  AACCAGGAGGCCAAGGGGGAAAGTGGTGTGGGCTGTGACAGCGGTGGCGGCAGCAGCAACAGCACTACCC
  ATAGCAGTGGCGAGGCAAGTTCTAGGCTGAGGGAGGAAGCCCAGCCCTCTGCACCTGAGCGCCTGGCCTT
  GGACTATATTGTGCCTTGCATGCGGTACTATGGTATCTGCGTCAAGGACAACTTCTTGGGGGCAGTACTG
  GGTGGCCGTGTGCTGGCTGAGGTGGAAGCCCTGAAGTGGGGCGGGCGTCTACGTGATGGGCAACTAGTGA
  GCCAGCGGGCGATCCCACCGCGCAGCATTCGTGGGGACCAGATTGCCTGGGTAGAAGGCCACGAGCCAGG
  CTGCCGGAGCATTGGTGCCCTCATGGCTCATGTGGACGCAGTAATCCGCCACTGTGCAGGGCGGCTGGGC
  AACTACGTCATCAATGGGCGCACCAAGGCCATGGTGGCGTGTTACCCAGGCAATGGGCTCGGGTACGTGA
  GGCATGTTGACAATCCCCACGGCGATGGGCGCTGCATCACCTGTATCTATTACCTGAATCAGAACTGGGA
  TGTTAAGGTGCATGGCGGCCTGCTGCAGATCTTCCCTGAGGGTCGGCCAGTGGTAGCCAACATCGAGCCA
  CTCTTTGACCGGTTGCTCATTTTCTGGTCTGACCGACGGAATCCACATGAGGTGAAGCCAGCCTATGCCA
  CCAGGTACGCCATCACTGTCTGGTATTTTGATGCCAAGGAGCGGGCAGCAGCCAGAGACAAGTATCAGCT
  AGCATCGGGACAGAAAGGTGTTCAAGTACCCGTGTCACAGCCAGCTACACCTACCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001004083
• Insert Size: 1248 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004083.1, NP_001004083.1
• RefSeq Size: 2013 bp
• RefSeq ORF: 1248 bp
• Locus ID: 308457
• Cytogenetics: 1q21
• Gene Summary: HIF-prolyl hydroxylase 1; might be involved in might play a role in hypoxic preconditioning [RGD, Feb 2006]