March7 (NM_001012087) Rat Untagged Clone

CAT#: RN213552

41340 (untagged ORF) - Rat membrane-associated ring finger (C3HC4) 7 (March7), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: March7
• Synonyms: Axot
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN213552 representing NM_001012087
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCTAAACCTTCCAGGATTCCAAGAAGAATTTCTGTTCAACCCTCTGGCTCTTTAAGTGCTAGGA
  TGGTATCTGGAAACCGAGGAACCAGTTTAAATGATTCCTATCATTCTAGAGACTCTTCCTTTAGACTGGA
  TTCTGAATATCAGTCTGCCTCAGCGTCAGCATCTGCATCACCATATCAGTCTACTTGGTACAGTGAGTCT
  GAGATAACTCAGGGAGCACGGTCACGGTCACAAAACCAGCAAAGGGATCATGATTCAAAAAGACCTAAAC
  TTTCTTGTACAAACTGTACGTCTACCTCAGCTGGGAGAAATATTGGAAGTGGTTTAAATACACTGTCAGA
  TTCTTCTTGGAGGCCTGGTCAAGTTCCGAGATCTTCTTCAATGGTCCTTGGTTCATTTGGAACAGACTTG
  ATGAGAGAGAGGAGAGATCTGGAGAGGAGAAGGGACTCCTCCATTAGCAGTCTTATGGACTATAGTCACC
  GGAGTGGGGATTTCACAGCTCCAGCATATGTTCAAGAAAGAGTTCCTTCTTCATATTCACAGGGAGCAAG
  ACCAAAAGAGAATGCCGCGAACACTTTACAGTTGAATTCATCCACCAATCACCAACTGCCTTCTGAGCAT
  CAGACAGTACCAAGTTCTAGGGACTCTAGTAGAAGTTCTTTCAGATCCCATTTTTCTCCAAGACAATCAG
  AATCTTTTCGAAACAGTTCACATCCTGCATTTTCATATCTTTCAAGTAGAAATGAAACTCCAACTATAAG
  CAGTTCAGAAAGGGCTGGCTCGTCTCAGAGACCATTTCAAGAATCTTCTGACAATGAAGGTAGGCGTACA
  ACTAGGAGATTGCTGTCACGGATAGCTTCTAGCATGTCATCTACCTTCTTTTCACGAAGATCTAGTCAAG
  ATTCCTTAAATACAAGGTCACTGAGTTCCGAAAATTATATTTCTCCAAGAACCCTGACTTCACAGTCTCG
  GAATAATGGAGCATCATCTTCTGAAGTTAATGATGGCAGGGCGTCTGAAGCGTCTCAGGGATTTAGGTTT
  CTTAGGCGAAGATGGGGTTTGTCGTCTCTTAGCCAGAATCATAGCTCTGAACCAGATGCAGAAAACTTTA
  ACCAAGAATCAGAAGGTAGAAATACAGGACCATGGTTGTCTTCCTCACTTAGAAATAGATGCACACCTTT
  GTTCTCTAGAAGGAGGCGAGAGGGAAGAGATGAGTCTTCAAGAATATCTCCATCAGATGTACCTCCTAGA
  TCTCATCTTTTCAGAAGAGAATCAAATGAAGTAGTTCACCTTGAAGCACAGGGGGACTCCCTTGGGGCAG
  CTGCCAGCAGACCACAAGCATCCGGAGCATCAGGCAATGCTTCTGCAAGTGGATCCACACCAGATTTGCC
  ACAGGGTGGAAGAAATACAGGAATAGCAGGGATCCTTCCTGGCTCCTTATTCCGGTTTGCAGTTCCGCCA
  GCACTTGGCAGTAACCTGACTGACAATGTCACGATTACCGTAGATATTATTCCTTCAGGTTGGAGTTCAG
  CTGATGGGAAAAGTGAAAAAGCTAAAAGTGCACCTTCAAGAGACCCAGAAAAACTTCAGAAAATCAAAGA
  AAGCCTCCTCTTAGAGGACTCTGATGAAGAAGAAGAAGGGGACTTATGTAGAATTTGCCAGATGGCAGCA
  GCGTCATCATCTAATTTATTGATAGAGCCATGCAAATGCACTGGGAGTCTGCAGTATGTCCATCAAGAGT
  GTATGAAGAAGTGGTTACAGGCCAAAATTAATTCTGGTTCTTCATTAGAGGCTGTAACCACCTGTGAACT
  CTGTAAAGAGAAGTTGCAACTTAACCTGGAGGATTTTGATATTCATGAACTACATAGAGCTCATGCAAAT
  GAACAAGCTGAGTATGAGTTTATCAGCTCTGGTCTCTACCTAGTTGTCTTACTGCACTTGTGTGAACAAA
  GCTTTTCTGATATGATGGGAAATACAATCGAACCAAGCACGCGCGTCCGATTTATTAACCTTGCAAGAAC
  TCTTCAGGCACATATGGAAGATCTCGAAACTTCAGAGGATGAGTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001012087
• Insert Size: 2079 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012087.1, NP_001012087.1
• RefSeq Size: 2421 bp
• RefSeq ORF: 2079 bp
• Locus ID: 311059
• Cytogenetics: 3q21
• Gene Summary: E3 ubiquitin-protein ligase which may specifically enhance the E2 activity of HIP2. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. [UniProtKB/Swiss-Prot Function]