Ppid (NM_001004279) Rat Untagged Clone

CAT#: RN214228

Ppid (untagged ORF) - Rat peptidylprolyl isomerase D (cyclophilin D) (Ppid), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Ppid
• Synonyms: Cyp-40; CypD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN214228 representing NM_001004279
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCACCCATCCCCAGCAGGCAAGCCCTCCAATTCCAAGAACCCGCGAGTCTTCTTTGACGTGGACA
  TCGGCGGGGAGCGAGTTGGACGAATTGTTTTAGAATTGTTTGCAGATATTGTTCCTAAAACTGCAGAAAA
  TTTTCGTGCATTGTGTACAGGAGAAAAGGGCACTGGACCGACAACTGGGAAACCTCTGCATTTTAAAGGA
  TGCCCTTTCCACCGAATTATTAAGAAATTTATGATTCAGGGTGGAGACTTCTCAAATCAGAATGGGACAG
  GTGGAGAAAGTATTTATGGTGAAAAATTTGAAGATGAGAATTTTCATTATAAGCATGATCGGGAGGGTTT
  GCTGAGCATGGCAAATGCAGGCCCCAATACAAATGGTTCTCAGTTCTTTATCACAACAGTTCCAACTCCT
  CATTTGGATGGGAAACATGTGGTATTTGGTCAAGTAATAAAAGGACTAGGTGTGGCAAGGATGCTTGAAA
  ATGTAGAAGTGAATGGTGAAAAACCTGCCAAACTCTGTGTTATTGCAGAATGTGGAGAATTGAAGGAAGG
  GGATGAATGGGGAATATTCCCCAAAGATGGCTCTGGGGATAGTCATCCAGATTTCCCTGAGGATGCAGAC
  ATAGACTTAAAGGATGTAGATAAAATTTTATTAATATCTGAAGACTTAAAAAACATTGGAAATACTTTTT
  TCAAGTCTCAAAACTGGGAGATGGCTATTAAAAAATATGCAAAGGTTTTAAGGTACTTGGATAGTTCAAA
  GGCTGTTATTGAGAAAGCAGATGTATCCAGACTGCAACCCATAGCCTTAAGTTGTGTGCTGAATATTGGT
  GCTTGTAAATTGAAGATGTCAAATTGGCAGGGGGCAATTGACAGTTGCTTGGAGGCTCTTGAAATGGACC
  CTTCAAACACTAAAGCACTATACCGAAAAGCACAAGGATGGCAAGGATTGAAAGAATATGATCAAGCATT
  GGCTGATCTTAAGAAGGCGCAGGAGATAGCCCCAGGAGATAAAGCTATCCAGGCAGAATTGCTTAAAGTC
  AAACAAATGATAAAGGCACAGAAAGATAAAGAGAAGGCAGTGTATGCAAAAATGTTTGCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001004279
• Insert Size: 1113 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004279.1, NP_001004279.1
• RefSeq Size: 1353 bp
• RefSeq ORF: 1113 bp
• Locus ID: 361967
• Cytogenetics: 2q33
• Gene Summary: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Proposed to act as a co-chaperone in HSP90 complexes such as in unligated steroid receptors heterocomplexes. Different co-chaperones seem to compete for association with HSP90 thus establishing distinct HSP90-co-chaperone-receptor complexes with the potential to exert tissue-specific receptor activity control. May have a preference for estrogen receptor complexes and is not found in glucocorticoid receptor complexes. May be involved in cytoplasmic dynein-dependent movement of the receptor from the cytoplasm to the nucleus. May regulate MYB by inhibiting its DNA-binding activity. Involved in regulation of AHR signaling by promoting the formation of the AHR:ARNT dimer; the function is independent of HSP90 but requires the chaperone activity region. Involved in regulation of UV radiation-induced apoptosis. [UniProtKB/Swiss-Prot Function]