Pou5f1 (NM_001009178) Rat Untagged Clone

CAT#: RN214935

Pou5f1/Oct4 (untagged ORF) - Rat POU class 5 homeobox 1 (Pou5f1/Oct4), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Pou5f1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN214935 representing NM_001009178
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGACACCTGGCTTCAGACTTCGCCTTCTCACCCCCACCTGGTGGGGGTGATGGGTCAGCAGGGC
  TGGAGCCGGGCTGGGTGGACCCTCGAACCTGGCTAAGCTTCCAGGGGCCTCCAAGTGGGCCTGGAATCGG
  ACCAGGTTCAGAGGTGCTGGGGATCTCCCCGTGTCCCCCAGCATACGAGTTCTGTGGAGGGATGGCATAC
  TGTGGACCTCAGGTTGGACTGGGCCTAGTCCCCCAAGTTGGCGTGGAGACTCTGCAGCCCGAGGGCCAGG
  CAGGAGCACGAGTGGAGAGCAACTCGGAGGGAGCCTCCTCTGGGCCCTGTACTGCCCGCCCCAGCGCCGT
  GAAGTTGGAGAAGGTGGAACCTAGTCCCGAGGAGTCCCAGGATATGAAAGCCCTGCAGAAGGAGCTAGAG
  CAGTTTGCCAAGCTGCTGAAACAGAAGAGGATCACCTTGGGGTACACCCAGGCCGACGTGGGGCTCACCC
  TGGGCGTTCTCTTTGGAAAGGTGTTCAGCCAGACAACCATCTGCCGCTTCGAGGCCCTGCAGCTCAGCCT
  TAAGAACATGTGTAAGCTGCGGCCCCTGCTGGAGAAGTGGGTGGAGGAAGCTGACAACAACGAGAACCTT
  CAGGAGATATGCAAATCGGAGACCCTGGTGCAGGCCCGGAAGAGAAAGCGGACTAGCATTGAGAACCGTG
  TGAGGTGGAACCTGGAGAACATGTTTCTGCAGTGCCCGAAGCCCTCCCTGCAGCAGATCACTAGCATTGC
  CAAGCAGCTTGGGCTGGAGAGGGATGTGGTTCGAGTGTGGTTCTGTAACCGGCGCCAGAAGGGGAAAAGA
  TCGAGCATTGAATATTCCCAACGAGAAGAGTATGAGGCCGCGGGGAAACCTTTCCCAGGGGGGGCTGTGT
  CCTTTCCTCTGCCCCCAGGCCCCCACTTTGGTGCTCCAGGCTATGGGAGCCCCCATTTCACCACACTCTA
  CTCGGTCCCTTTTCCTGAGGGCGAGGCCTTTCCCTCTGTTCCTGTCACTGCTCTGGGCTCTCCCATGCAT
  TCAAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001009178
• Insert Size: 1059 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009178.2, NP_001009178.1
• RefSeq Size: 1388 bp
• RefSeq ORF: 1059 bp
• Locus ID: 294562
• Cytogenetics: 20p12