Dio3 (NM_017210) Rat Untagged Clone

CAT#: RN215406

Dio3 (untagged ORF) - Rat deiodinase, iodothyronine, type III (Dio3), (Note, selenocysteine protein, internal stop codon, see reference data summary)

Specifications

Product Data

• Type: Rat Untagged Clone
• Symbol: Dio3
• Synonyms: 5DIII; DIOIII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN215406 representing NM_017210
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCGCCAGGCCGCCTCGAGGTTGGTGGTCGGAGAAGGTGAAGGGCCCCCGGGGGCTTCGGGGCCCG
  CGGCCACCATGCTGCGCTCCCTGCTGCTTCACTCTCTGAGGCTCTGCGCCCAGACCGCCTCGTGCCTCGT
  GCTGTTCCCGCGCTTCCTAGGCACGGCCTTCATGCTCTGGCTTTTAGATTTCTTGTGCATCCGCAAGCAT
  TTCCTGCGCCGTCGTCATCCTGACCACCCCGAGCCCGAAGTAGAGCTCAACAGTGAAGGTGAGGAGATGC
  CCCCTGACGACCCACCCATCTGCGTATCCGACGACAACCGGCTGTGCACCCTGGCCTCTCTGAAGGCCGT
  GTGGCATGGCCAGAAGTTGGATTTCTTCAAGCAGGCCCATGAGGGTGGCCCAGCACCTAACTCGGAGGTC
  GTCCGGCCTGATGGCTTCCAGAGCCAGCGCATCCTCGACTACGCACAAGGGACCCGCCCGTTGGTGCTCA
  ATTTTGGCAGCTGTACCTGACCACCGTTCATGGCGCGGATGAGCGCCTTCCAGCGCCTGGTCACCAAGTA
  CCAGCGCGACGTTGACTTCCTTATCATCTACATCGAGGAAGCCCACCCATCCGATGGCTGGGTCACCACA
  GATTCACCCTACGTCATCCCACAGCACCGCAGCTTGGAGGACCGTGTCAGCGCAGCAAGAGTACTTCAGC
  AAGGCGCACCTGGCTGTGCTCTGGTTCTGGACACGATGGCCAACTCCAGCAGTTCCGCATATGGTGCCTA
  TTTTGAGCGCCTCTACGTCATCCAGAGCGGCACCATCATGTACCAGGGAGGCCGTGGCCCCGACGGTTAC
  CAGGTGTCTGAGTTGCGCACTTGGTTGGAGCGCTATGACGAGCAGTTGCATGGTACTAGGCCACGTCGAC
  TCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_017210
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.

Reference Data

• RefSeq: NM_017210.4, NP_058906.3
• RefSeq Size: 1862
• RefSeq ORF: 915
• Locus ID: 29475
• Gene Summary: This is an intronless, imprinted gene that is preferentially expressed from the paternal allele. The encoded protein belongs to the iodothyronine deiodinase family, and catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5' triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. It is highly expressed in placenta, fetal and neonatal tissues, and thought to prevent premature exposure of developing fetal tissues to adult levels of thyroid hormones. It thus plays a critical role in mammalian development by regulating circulating fetal thyroid hormone concentration. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jun 2016]