Azin1 (NM_001301702) Rat Untagged Clone

CAT#: RN216695

Azin1 (untagged) - Rat antizyme inhibitor 1 (Azin1), transcript variant 2

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Azin1
• Synonyms: Oazi; Oazin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216695 representing NM_001301702
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGGATTTATTGACGATGCAAACTACTCCGTTGGCCTGTTGGATGAAGGAACAAACCTTGGAAATG
  TTATTGATAACTATGTTTATGAACATACCCTGACAGGAAAAAATGCATTTTTTGTGGGGGATCTTGGGAA
  GATCGTGAAGAAGCACAGTCAGTGGCAGAACGTGGTGGCTCAGATAAAGCCATTTTACATGGTGAAGTGC
  AACTCCACTCCAGCCGTACTGGAGATCTTGGCAGCTCTTGGAACTGGATTTGCTTGTTCCAGCAAAAATG
  AAATGGCTTTAGTGCAAGAATTAGGCGTATCTCCAGAAAACATCATTTATACAAGTCCTTGTAAGCAAGC
  ATCTCAGATAAAGTACGCAGCAAAAGTTGGAGTAAATATTATGACATGTGACAATGAAGTTGAATTAAAG
  AAAATTGCGAGGAATCACCCAAATGCCAAGGTCTTACTACATATTGCAACAGAAGATAATATTGGAGGTG
  AAGATGGTAATATGAAGTTTGGCACTACACTGAAGAACTGTAGGCATCTTTTGGAATGTGCCAAGGAACT
  TGATGTCCAAATAATTGGGGTTAAATTTCATATTTCAAGTGCTTGCAAAGAATATCAAGTCTATGTCCAT
  GCCCTGTCTGATGCTCGATGTGTGTTTGACATGGCTGGGGAGTTTGGCTTTACAATGAACATGTTAGACA
  TCGGTGGAGGCTTCACAGGAACTGAAATTCAGTTGGAAGAGGTTAATCATGTTATCAGTCCTCTGTTGGA
  TATTTACTTCCCTGAAGGATCTGGCATTCAGATCATTTCCGAACCTGGAAGCTACTATGTATCTTCTGCA
  TTTACGCTTGCAGTCAATATTATTGCTAAGAAAGTTGTTGAAAATGATAAATTATCCTCTGGAGTAGAAA
  AAAATGGGAGTGACGAGCCAGCCTTCGTGTACTACATGAATGACGGTGTTTATGGTTCTTTTGCAAGTAA
  GCTTTCTGAGGACTTAAATACTGTTCCAGAGGTTCACAAGAAATACAAGGAAGATGAGCCTCTGTTTACA
  AGCAGCCTTTGGGGTCCATCCTGTGACGAGCTTGATCAAATTGTGGAAAGTTGTCTTCTTCCTGAGCTGA
  GCGTGGGAGATTGGCTTATCTTTGATAACATGGGAGCAGATTCTTTGCACGGACCGTCTGCTTTTAGTGA
  CACTCAGAGGCCAGCTATTTATTTCATGATGTCACTCAGTGATTGGTATGAGATGCAAGATGCTGGAATT
  ACTTCAGATGCAATGATGAAAAACTTCTTCTTTGCACCCTCTTGCATTCAGCTGAGCCAAGAAGACAACT
  TCTCCACTGAAGCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001301702
• ORF Size: 1347 bp
• Insert Size: 1347
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001301702.1, NP_001288631.1
• RefSeq Size: 4186
• RefSeq ORF: 1347
• Locus ID: 58961
• Gene Summary: The protein encoded by this gene belongs to the antizyme inhibitor family, which plays a role in cell growth and proliferation by maintaining polyamine homeostasis within the cell. Antizyme inhibitors are homologs of ornithine decarboxylase (ODC, the key enzyme in polyamine biosynthesis) that have lost the ability to decarboxylase ornithine; however, retain the ability to bind to antizymes. Antizymes negatively regulate intracellular polyamine levels by binding to ODC and targeting it for degradation, as well as by inhibiting polyamine uptake. Antizyme inhibitors function as positive regulators of polyamine levels by sequestering antizymes and neutralizing their effect. This gene encodes antizyme inhibitor 1, the first member of this gene family that is ubiquitously expressed, and is localized in the nucleus and cytoplasm. Overexpression of antizyme inhibitor 1 gene has been associated with increased proliferation, cellular transformation and tumorigenesis. Gene knockout studies showed that homozygous mutant mice lacking functional antizyme inhibitor 1 gene died at birth with abnormal liver morphology. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014]
  Transcript Variant: This variant (2) lacks an internal 5' non-coding exon, thus has a shorter 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.