Gck (NM_001270850) Rat Untagged Clone

CAT#: RN216698

Gck (untagged) - Rat glucokinase (Gck), transcript variant 3

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Gck
• Synonyms: GLK; GLUKA; RNGK2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216698 representing NM_001270850
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGATGACAGAGCCAGGATGGAGGCCACCAAGAAGGAAAAGGTCGAGCAGATCCTGGCAGAGTTCC
  AGCTGCAGGAGGAAGACCTGAAGAAGGTGATGAGCCGGATGCAGAAGGAGATGGACCGTGGCCTGAGGCT
  GGAGACCCACGAGGAGGCCAGTGTAAAGATGTTACCCACCTACGTGCGTTCCACCCCAGAAGGCTCAGAA
  GTCGGAGACTTTCTCTCCTTAGACCTGGGAGGAACCAACTTCAGAGTGATGCTGGTCAAAGTGGGAGAGG
  GGGAGGCAGGGCAGTGGAGCGTGAAGACAAAACACCAGATGTACTCCATCCCCGAGGACGCCATGACGGG
  CACTGCCGAGATGATGAAGCACAAGAAACTGCCCCTGGGCTTCACCTTCTCCTTCCCTGTGAGGCACGAA
  GACCTAGACAAGGGCATCCTCCTCAATTGGACCAAGGGCTTCAAGGCCTCTGGAGCAGAAGGGAACAACA
  TCGTAGGACTTCTCCGAGATGCTATCAAGAGGAGAGGGGACTTTGAGATGGATGTGGTGGCAATGGTGAA
  CGACACAGTGGCCACAATGATCTCCTGCTACTATGAAGACCGCCAATGTGAGGTCGGCATGATTGTGGGC
  ACTGGCTGCAATGCCTGCTACATGGAGGAAATGCAGAATGTGGAGCTGGTGGAAGGGGATGAGGGACGCA
  TGTGCGTCAACACGGAGTGGGGCGCCTTCGGGGACTCGGGCGAGCTGGATGAGTTCCTACTGGAGTATGA
  CCGGATGGTGGATGAAAGCTCAGCGAACCCCGGTCAGCAGCTGTACGAGAAGATCATCGGTGGGAAGTAT
  ATGGGCGAGCTGGTACGACTTGTGCTGCTTAAGCTGGTGGACGAGAACCTTCTGTTCCACGGAGAGGCCT
  CGGAGCAGCTGCGCACGCGTGGTGCTTTTGAGACCCGTTTCGTGTCACAAGTGGAGAGCGACTCCGGGGA
  CCGAAAGCAGATCCACAACATCCTAAGCACTCTGGGGCTTCGACCCTCTGTCACCGACTGCGACATTGTG
  CGCCGTGCCTGTGAAAGCGTGTCCACTCGCGCCGCCCATATGTGCTCCGCAGGACTAGCTGGGGTCATAA
  ATCGCATGCGCGAAAGCCGCAGTGAGGACGTGATGCGCATCACTGTGGGCGTGGATGGCTCCGTGTACAA
  GCTGCACCCGAGCTTCAAGGAGCGGTTTCACGCCAGTGTGCGCAGGCTGACACCCAACTGCGAAATCACC
  TTCATCGAATCAGAGGAGGGCAGCGGCAGGGGAGCCGCGCTGGTCTCTGCGGTGGCCTGCAAGAAGGCTT
  GCATGCTGGCCCAGTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001270850
• ORF Size: 1347 bp
• Insert Size: 1347
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001270850.1, NP_001257779.1
• RefSeq Size: 2220
• RefSeq ORF: 1347
• Locus ID: 24385
• Gene Summary: catalyzes the conversion of ATP and D-glucose to ADP and D-glucose 6-phosphate; may mediate glucosensing in glucose-inhibited and glucose-excited neurons [RGD, Feb 2006]
  Transcript Variant: This variant (3) differs in the 5' UTR and coding region and uses two alternate splice junctions compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus and contains two alternate internal segments compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.