Gck (NM_001270850) Rat Tagged ORF Clone

CAT#: RR216698

Gck (myc-DDK-tagged) - Rat glucokinase (Gck), transcript variant 3

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gck
• Synonyms: GLK; GLUKA; RNGK2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216698 representing NM_001270850
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGATGACAGAGCCAGGATGGAGGCCACCAAGAAGGAAAAGGTCGAGCAGATCCTGGCAGAGTTCC
  AGCTGCAGGAGGAAGACCTGAAGAAGGTGATGAGCCGGATGCAGAAGGAGATGGACCGTGGCCTGAGGCT
  GGAGACCCACGAGGAGGCCAGTGTAAAGATGTTACCCACCTACGTGCGTTCCACCCCAGAAGGCTCAGAA
  GTCGGAGACTTTCTCTCCTTAGACCTGGGAGGAACCAACTTCAGAGTGATGCTGGTCAAAGTGGGAGAGG
  GGGAGGCAGGGCAGTGGAGCGTGAAGACAAAACACCAGATGTACTCCATCCCCGAGGACGCCATGACGGG
  CACTGCCGAGATGATGAAGCACAAGAAACTGCCCCTGGGCTTCACCTTCTCCTTCCCTGTGAGGCACGAA
  GACCTAGACAAGGGCATCCTCCTCAATTGGACCAAGGGCTTCAAGGCCTCTGGAGCAGAAGGGAACAACA
  TCGTAGGACTTCTCCGAGATGCTATCAAGAGGAGAGGGGACTTTGAGATGGATGTGGTGGCAATGGTGAA
  CGACACAGTGGCCACAATGATCTCCTGCTACTATGAAGACCGCCAATGTGAGGTCGGCATGATTGTGGGC
  ACTGGCTGCAATGCCTGCTACATGGAGGAAATGCAGAATGTGGAGCTGGTGGAAGGGGATGAGGGACGCA
  TGTGCGTCAACACGGAGTGGGGCGCCTTCGGGGACTCGGGCGAGCTGGATGAGTTCCTACTGGAGTATGA
  CCGGATGGTGGATGAAAGCTCAGCGAACCCCGGTCAGCAGCTGTACGAGAAGATCATCGGTGGGAAGTAT
  ATGGGCGAGCTGGTACGACTTGTGCTGCTTAAGCTGGTGGACGAGAACCTTCTGTTCCACGGAGAGGCCT
  CGGAGCAGCTGCGCACGCGTGGTGCTTTTGAGACCCGTTTCGTGTCACAAGTGGAGAGCGACTCCGGGGA
  CCGAAAGCAGATCCACAACATCCTAAGCACTCTGGGGCTTCGACCCTCTGTCACCGACTGCGACATTGTG
  CGCCGTGCCTGTGAAAGCGTGTCCACTCGCGCCGCCCATATGTGCTCCGCAGGACTAGCTGGGGTCATAA
  ATCGCATGCGCGAAAGCCGCAGTGAGGACGTGATGCGCATCACTGTGGGCGTGGATGGCTCCGTGTACAA
  GCTGCACCCGAGCTTCAAGGAGCGGTTTCACGCCAGTGTGCGCAGGCTGACACCCAACTGCGAAATCACC
  TTCATCGAATCAGAGGAGGGCAGCGGCAGGGGAGCCGCGCTGGTCTCTGCGGTGGCCTGCAAGAAGGCTT
  GCATGCTGGCCCAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216698 representing NM_001270850
  Red=Cloning site Green=Tags(s)
  
  MLDDRARMEATKKEKVEQILAEFQLQEEDLKKVMSRMQKEMDRGLRLETHEEASVKMLPTYVRSTPEGSE
  VGDFLSLDLGGTNFRVMLVKVGEGEAGQWSVKTKHQMYSIPEDAMTGTAEMMKHKKLPLGFTFSFPVRHE
  DLDKGILLNWTKGFKASGAEGNNIVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDRQCEVGMIVG
  TGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRMVDESSANPGQQLYEKIIGGKY
  MGELVRLVLLKLVDENLLFHGEASEQLRTRGAFETRFVSQVESDSGDRKQIHNILSTLGLRPSVTDCDIV
  RRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPNCEIT
  FIESEEGSGRGAALVSAVACKKACMLAQ
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270850
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001270850.1, NP_001257779.1
• RefSeq Size: 2220
• RefSeq ORF: 1347
• Locus ID: 24385
• MW: 50.1 kDa
• Gene Summary: catalyzes the conversion of ATP and D-glucose to ADP and D-glucose 6-phosphate; may mediate glucosensing in glucose-inhibited and glucose-excited neurons [RGD, Feb 2006]