Ephx1 (NM_001034090) Rat Untagged Clone

CAT#: RN216705

Ephx1 (untagged) - Rat epoxide hydrolase 1, microsomal (xenobiotic) (Ephx1), transcript variant 1

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Ephx1
• Synonyms: mEH; MEH8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216705 representing NM_001034090
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCTGGAACTTGTCCTGGCTTCCCTTCTGGGCTTTGTCATCTACTGGTTTGTCTCCCGGGACAAGG
  AGGAAACCTTACCACTAGGAGATGGATGGTGGGGGCCAGGGTCAAAGCCATCAGCCAAAGAAGATGAGAG
  CATCCGGCCCTTCAAGGTGGAAACATCAGATGAGGAGATCAAGGACTTACACCAGAGGATAGATAGGTTC
  CGGGCATCCCCACCTTTGGAGGGCAGCCGCTTCCACTATGGCTTCAACTCCAACTACATGAAGAAAGTGG
  TGTCCTACTGGAGGAACGAGTTTGACTGGAGGAAGCAGGTGGAGATCCTCAACCAGTACCCTCACTTCAA
  GACCAAGATCGAAGGGCTTGACATCCACTTCATCCATGTGAAGCCTCCCCAGCTGCCCTCAGGGCGCACC
  CCAAAGCCCTTGCTGATGGTGCATGGCTGGCCTGGATCCTTCTATGAGTTTTACAAGATCATCCCACTAC
  TGACTGACCCCAAGTCCCACGGTCTGAGTGACGAGCACGTGTTTGAAGTCATCTGTCCCTCGATTCCTGG
  CTATGGCTACTCAGAGGCATCCAGCAAGAAAGGTTTAAATTCGGTGGCCACTGCGAGGATTTTCTACAAG
  CTGATGACACGGCTGGGCTTCCAGAAATTCTACATTCAAGGCGGGGACTGGGGGTCCCTCATCTGCACCA
  ACATGGCCCAGATGGTTCCCAACCACGTGAAAGGCCTGCACTTAAATATGGCTTTCATTTCGAGAAGTTT
  TTACACCATGACTCCTCTCCTGGGCCAACGCTTCGGGAGATTCCTTGGCTACACAGAGAAGGATATCGAG
  CTCTTGTACCCCTATAAGGAGAAGGTTTTCTACAGCATCATGAGGGAGAGTGGCTACTTACACATCCAAG
  CCACCAAGCCAGACACTGTGGGCTGTGCTCTCAATGACTCTCCCGTGGGCCTGGCTGCCTACATCTTAGA
  GAAGTTCTCCACCTGGACCAAGTCAGAGTACCGTGAACTGGAGGATGGAGGCCTGGAGAGGAAGTTCTCC
  CTGGATGATCTGCTGGTTAACATCATGATCTACTGGACGACAGGAACCATTGTCTCCTCCCAACGCTACT
  ACAAGGAGAATTTGGGCCAGGGCATCATGGTCCATAAACATGAGGGGATGAAGGTCTTTGTGCCCACTGG
  CTTTTCAGCCTTCCCTTCCGAGCTACTGCATGCCCCAGAAAAGTGGGTGAAGGTCAAGTACCCCAAACTC
  ATCTCCTATTCCTACATGGAACGTGGGGGCCACTTTGCTGCCTTTGAAGAGCCCAAGCTTCTGGCCCAGG
  ACATCCGCAAGTTCGTGTCCCTGGCTGAGCTGCAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001034090
• Insert Size: 1368 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034090.3, NP_001029262.1
• RefSeq Size: 1732 bp
• RefSeq ORF: 1368 bp
• Locus ID: 25315
• Cytogenetics: 13q26
• Gene Summary: catalyzes the hydration of a wide range of xenobiotic arene oxides [RGD, Feb 2006]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.