Sphk1 (NM_001270811) Rat Untagged Clone

CAT#: RN216714

Sphk1 (untagged) - Rat sphingosine kinase 1 (Sphk1), transcript variant 1

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Sphk1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216714 representing NM_001270811
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAACTGGACTCTCCACCAACCTCAAAGGATCTGGCCGCCTCCGGGAATGACGAGGGCGCTCTCACAG
  CCCAGGGAGGTGGAGAGGGCGAGCCCCACGGCCAGTCACCAGACACCCGCCTGGGCAACACCGATAAGCA
  GCTGCACACTGGAGCCGCCGTTACCTCTAGCAGCGCCGGGGCAGCATCGGTGGCTCCTTGTCAGCGGGAG
  CCCCGGGACCTGGCTATGCAACCAGCAGACTGTCCCCGGGGACTGCTCCCACGGCCATGCAGAGTGTTGG
  TGCTGCTGAACCCCCGGGGTGGCAAGGGCAAGGCTCTGAAGCTCTTTCAGAGCCGTGTGCGGCCCCTTCT
  GGAGGAGGCTGAGGTATCCTTTAAACTGATGCTCACCGAACGGCAGAACCATGCCAGGGAGCTGGTGTGT
  GCAGAAGAACTGGGTCACTGGGATGCCTTGGCGGTCATGTCCGGTGACGGTCTGATGCACGAGGTGGTGA
  ATGGGCTAATGGAACGACCTGACTGGGAGAGTGCCATCCAGAAACCCCTATGTAGCCTCCCTGGAGGCTC
  TGGCAATGCGCTGGCAGCTTCTTTGAACTACTATGCTGGGCACGAGCAGGTCACTAATGAAGATCTCCTG
  ATCAACTGCACACTGCTGTTATGCTGCCGGCAGCTGTCACCCATGAATCTGCTGTCCCTGCACACCGCTT
  CCGGACGGCAACTCTATTCTGTTCTCAGTCTGTCCTGGGGTTTCGTTGCTGACGTGGACTTGGAGAGTGA
  GAAATACAGGAGCTTGGGGGAGATTCGTTTCACGGTGGGAACCTTTTTTCGCCTAGCAAGCCTGCGCATT
  TACCAAGGCCAACTGGCCTACCTTCCTGTAGGGAAGGCTGCCTCTAAGATACCTGCTTCCTCACTGGCAC
  AGAAGGGCCCTGCCAACACATACCTCGTTCCTCTGGAGGAGCCAGTGCCACCTCATTGGACTGTGGTGCC
  AGAGCAGGACTTCGTTCTGGTGCTGGTGCTGCTTCACACCCACCTGAGCACAGAGATGTTTGCAGCACCC
  ATGGGCCGCTGCGAGGCTGGTGTTATGCATCTGTTCTATATACGTGCGGGGGTGTCAAGGGCCATGCTGC
  TGCGCCTCTTCCTGGCCATGCAGAAGGGCAAGCATATGGACCTCGACTGCCCGTACTTGGTTCATGTGCC
  AGTGGTTGCCTTCCGCCTGGAACCCAGGAACCAGAGGGGAGTGTTTTCTGTGGACGGGGAGCTGATGGTG
  TGTGAAGCTGTGCAGGGCCAAGTGCACCCAAACTACCTTTGGATGGTCTCTGGTAGCAGTGACTCCCCGT
  CCGGCCGGGACTCCCAGCGGAGGCCACCTCCAGAAGAACCAATATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001270811
• ORF Size: 1377 bp
• Insert Size: 1377
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001270811.1, NP_001257740.1
• RefSeq Size: 2915
• RefSeq ORF: 1377
• Locus ID: 170897
• Gene Summary: catalyzes the conversion of sphingosine to sphingosine 1 phosphate, which plays a role in cell proliferation and differentiation [RGD, Feb 2006]
  Transcript Variant: This variant (1) encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.