Sphk1 (NM_001270811) Rat Tagged ORF Clone

CAT#: RR216714

Sphk1 (myc-DDK-tagged) - Rat sphingosine kinase 1 (Sphk1), transcript variant 1

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sphk1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216714 representing NM_001270811
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAACTGGACTCTCCACCAACCTCAAAGGATCTGGCCGCCTCCGGGAATGACGAGGGCGCTCTCACAG
  CCCAGGGAGGTGGAGAGGGCGAGCCCCACGGCCAGTCACCAGACACCCGCCTGGGCAACACCGATAAGCA
  GCTGCACACTGGAGCCGCCGTTACCTCTAGCAGCGCCGGGGCAGCATCGGTGGCTCCTTGTCAGCGGGAG
  CCCCGGGACCTGGCTATGCAACCAGCAGACTGTCCCCGGGGACTGCTCCCACGGCCATGCAGAGTGTTGG
  TGCTGCTGAACCCCCGGGGTGGCAAGGGCAAGGCTCTGAAGCTCTTTCAGAGCCGTGTGCGGCCCCTTCT
  GGAGGAGGCTGAGGTATCCTTTAAACTGATGCTCACCGAACGGCAGAACCATGCCAGGGAGCTGGTGTGT
  GCAGAAGAACTGGGTCACTGGGATGCCTTGGCGGTCATGTCCGGTGACGGTCTGATGCACGAGGTGGTGA
  ATGGGCTAATGGAACGACCTGACTGGGAGAGTGCCATCCAGAAACCCCTATGTAGCCTCCCTGGAGGCTC
  TGGCAATGCGCTGGCAGCTTCTTTGAACTACTATGCTGGGCACGAGCAGGTCACTAATGAAGATCTCCTG
  ATCAACTGCACACTGCTGTTATGCTGCCGGCAGCTGTCACCCATGAATCTGCTGTCCCTGCACACCGCTT
  CCGGACGGCAACTCTATTCTGTTCTCAGTCTGTCCTGGGGTTTCGTTGCTGACGTGGACTTGGAGAGTGA
  GAAATACAGGAGCTTGGGGGAGATTCGTTTCACGGTGGGAACCTTTTTTCGCCTAGCAAGCCTGCGCATT
  TACCAAGGCCAACTGGCCTACCTTCCTGTAGGGAAGGCTGCCTCTAAGATACCTGCTTCCTCACTGGCAC
  AGAAGGGCCCTGCCAACACATACCTCGTTCCTCTGGAGGAGCCAGTGCCACCTCATTGGACTGTGGTGCC
  AGAGCAGGACTTCGTTCTGGTGCTGGTGCTGCTTCACACCCACCTGAGCACAGAGATGTTTGCAGCACCC
  ATGGGCCGCTGCGAGGCTGGTGTTATGCATCTGTTCTATATACGTGCGGGGGTGTCAAGGGCCATGCTGC
  TGCGCCTCTTCCTGGCCATGCAGAAGGGCAAGCATATGGACCTCGACTGCCCGTACTTGGTTCATGTGCC
  AGTGGTTGCCTTCCGCCTGGAACCCAGGAACCAGAGGGGAGTGTTTTCTGTGGACGGGGAGCTGATGGTG
  TGTGAAGCTGTGCAGGGCCAAGTGCACCCAAACTACCTTTGGATGGTCTCTGGTAGCAGTGACTCCCCGT
  CCGGCCGGGACTCCCAGCGGAGGCCACCTCCAGAAGAACCAATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216714 representing NM_001270811
  Red=Cloning site Green=Tags(s)
  
  MQLDSPPTSKDLAASGNDEGALTAQGGGEGEPHGQSPDTRLGNTDKQLHTGAAVTSSSAGAASVAPCQRE
  PRDLAMQPADCPRGLLPRPCRVLVLLNPRGGKGKALKLFQSRVRPLLEEAEVSFKLMLTERQNHARELVC
  AEELGHWDALAVMSGDGLMHEVVNGLMERPDWESAIQKPLCSLPGGSGNALAASLNYYAGHEQVTNEDLL
  INCTLLLCCRQLSPMNLLSLHTASGRQLYSVLSLSWGFVADVDLESEKYRSLGEIRFTVGTFFRLASLRI
  YQGQLAYLPVGKAASKIPASSLAQKGPANTYLVPLEEPVPPHWTVVPEQDFVLVLVLLHTHLSTEMFAAP
  MGRCEAGVMHLFYIRAGVSRAMLLRLFLAMQKGKHMDLDCPYLVHVPVVAFRLEPRNQRGVFSVDGELMV
  CEAVQGQVHPNYLWMVSGSSDSPSGRDSQRRPPPEEPI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270811
• ORF Size: 1374 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001270811.1, NP_001257740.1
• RefSeq Size: 2915
• RefSeq ORF: 1377
• Locus ID: 170897
• MW: 49.9 kDa
• Gene Summary: catalyzes the conversion of sphingosine to sphingosine 1 phosphate, which plays a role in cell proliferation and differentiation [RGD, Feb 2006]