Mapk10 (NM_001270556) Rat Untagged Clone

CAT#: RN216728

Mapk10 (untagged) - Rat mitogen activated protein kinase 10 (Mapk10), transcript variant 1

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Mapk10
• Synonyms: Jnk3; SAPb; SAPKC; Serk2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216728 representing NM_001270556
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCTCCATTTCTTATACTACTGCAGTGAACCAACCTTGGATGTGAAAATTGCCTTTTGTCAGGGAT
  TCGACAAACACGTGGATGTGTCTTCTGTTGCCAAACATTACAACATGAGCAAAAGCAAGGTAGATAACCA
  GTTCTACAGTGTGGAAGTGGGAGACTCAACCTTCACAGTTCTAAAGCGCTACCAGAACCTGAAGCCGATC
  GGCTCTGGGGCTCAGGGAATAGTTTGTGCTGCGTATGACGCTGTCCTCGACAGAAATGTGGCCATTAAGA
  AGCTCAGCAGACCCTTCCAGAACCAAACTCATGCCAAGAGGGCTTACCGGGAGCTGGTCCTCATGAAGTG
  TGTGAACCATAAAAACATTATTAGCTTATTAAATGTCTTTACACCCCAGAAAACACTGGAGGAGTTCCAA
  GATGTTTACTTAGTGATGGAACTGATGGACGCCAACTTGTGTCAGGTGATTCAGATGGAGCTGGACCACG
  AGCGGATGTCGTACTTGCTGTACCAGATGCTGTGCGGCATCAAACACCTCCACTCCGCTGGGATCATCCA
  CAGGGACTTAAAACCCAGTAACATCGTAGTCAAGTCTGATTGCACACTGAAAATCCTGGACTTTGGACTG
  GCCAGGACAGCGGGCACAAGCTTCATGATGACTCCGTATGTGGTGACGAGATATTACAGAGCCCCCGAGG
  TCATCCTGGGCATGGGCTACAAGGAGAACGTGGACATATGGTCTGTGGGCTGCATCATGGGAGAAATGGT
  TCGTCACAAAATCCTCTTTCCCGGAAGGGACTATATTGACCAGTGGAACAAAGTCATAGAGCAGCTAGGA
  ACTCCGTGTCCAGAATTCATGAAGAAATTGCAGCCCACCGTCAGAAACTACGTGGAGAACCGGCCCAAGT
  ATGCAGGCCTCACCTTCCCCAAGCTCTTTCCAGATTCCCTCTTCCCAGCGGATTCCGAGCACAATAAACT
  TAAAGCCAGCCAAGCCAGGGACTTGTTGTCAAAGATGTTAGTGATTGACCCAGCGAAGAGGATATCGGTG
  GATGACGCATTGCAGCATCCGTACATCAACGTTTGGTACGACCCTGCTGAAGTGGAGGCGCCTCCGCCTC
  AGATATATGACAAGCAACTGGATGAAAGGGAGCACACCATCGAAGAATGGAAAGAACTCATCTACAAGGA
  AGTAATGAACTCAGAAGAGAAGACTAAGAACGGCGTAGTCAAAGGCCAGCCCTCACCTTCAGGTGCAGCA
  GTGAACAGCAGTGAGAGTCTCCCTCCATCCTCGTCTGTCAACGACATCTCCTCCATGTCCACCGACCAGA
  CCCTCGCATCCGACACTGACAGCAGCCTGGAAGCCTCGGCGGGACCGCTGGGTTGTTGCAGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001270556
• ORF Size: 1395 bp
• Insert Size: 1395
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001270556.1, NP_001257485.1
• RefSeq Size: 2759
• RefSeq ORF: 1395
• Locus ID: 25272
• Gene Summary: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as integration points for multiple biochemical signals, and thus are involved in a wide variety of cellular processes, such as proliferation, differentiation, transcription regulation and development. This kinase is specifically expressed in a subset of neurons in the nervous system and is activated by threonine and tyrosine phosphorylation. Targeted deletion of this gene in mice suggests that it may have a role in stress-induced neuronal apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2017]
  Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the shorter isoform (1).