Slc19a1 (NM_001035232) Rat Untagged Clone

CAT#: RN216818

Slc19a1 (untagged) - Rat solute carrier family 19 (folate transporter), member 1 (Slc19a1), transcript variant 2

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Slc19a1
• Synonyms: MTX1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216818 representing NM_001035232
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCCCACTGGCCAGGTGGCAGAAAAGCAAGCCTGCGAAGAGCCCAGGCAGGACCGAGAACTTAAGT
  CCTGGCGATGGCTCGTGTTCTACCTCTGCTTCTTTGGCTTCATGGCACAGTTGCGACCTGGGGAGAGCTT
  CATCACACCCTACCTCCTGGAACGTAACTTCACCAAGGAGCAGGTGACCAACGAGATCATTCCGATGCTG
  CCCTACTCCCACCTGGCCGTGCTGGTCCCCATCTTCCTGCTCACCGACTACCTACGATACAAGCCAGTCC
  TGGTCCTACAGTGCCTGAGCTTCGTGTGCGTGTGGCTGCTGCTGCTCCTGGGGACCTCCGTCGTGCACAT
  GCAGCTCATGGAAGTCTTCTACAGCATCACCATGGCGGCGCGCATCGCCTACTCCTCCTACATATTCTCC
  CTGGTGCAGCCCTCCCGCTACCAGCGCATGGCCAGCTACTCGCGGGCCGCAGTCCTACTGGGCGTCTTCA
  TCAGCTCCGTGCTGGGCCAGGTGCTGGTCACCCTAGGAGGCATCTCCACCTATATGCTCAACTGTATCTC
  CCTGGGCTTCATCCTCTTCAGCCTGAGCCTCTCCCTCTTCCTAAAGCGTCCTAAGCGGAGCCTCTTTTTC
  AACCGGAGCGCACTTGTGCAAGGAGCCTTGCCCTGTGAGCTGGATCAGATGCACCCGGGACCTGGCCGTC
  CAGAGCCCAGGAAACTGGAGCGCATGCTGGGCACTTGCAGGGACTCCTTCCTGGTACGCATGCTAAGCGA
  ACTGGTGAAGAACGTCCGGCAACCACAGCTGCGTCTCTGGTGCCTCTGGTGGGTCTTCAACTCGGCAGGC
  TACTACCTGATCACCTACTATGTCCATGTCCTGTGGAAAATCACTGACAGCAGACTCAACTACAACGGTG
  CCGTAGATGCCGCCTCCACACTTCTGAGCGCCATCACGGCCTTCACCGCGGGCTTCGTGAACATCCGCTG
  GGCTCTGTGGTCAAAGCTGGTCATCGCGAGTGTCATAGCCATCCAGGCTGGCCTGGTTTTCTGTATGTTC
  CAGATCCCGGACATCTGGGTCTGCTATGTGACCTTCGTGCTTTTCCGTGGGGCCTACCAGTTCCTTGTGC
  CCATTGCCACTTTTCAGATTGCATCTTCCCTGTCTAAAGAGCTCTGTGCATTGGTCTTCGGGATCAACAC
  TTTCCTTGCCACTGCGCTTAAGACCTCGATCACACTCGTGGTCTCTGACAAGCGGGGTCTGGGCCTCCAA
  GTCCATCAACAGTTCCGCATCTACTTCATGTACTTCCTGACGCTGTCCATCATCTGCCTTGCCTGGGCTG
  GGCTGGACGGCTTGAGGTACTACCGGCGTGGTCGCCACCAGCCGCTGGCCCAGGCCCAGGCTCTGAGTCC
  CTTGGAGGATTCTGTGCAGGCGATAAGCCTACAGGATGGAGACCTAAGGAGACCGCAGCCCTCGGCACCA
  CAGCTGCTCCCAGAGGATGGCAGCGTAGAAGACGGCAGGGCAGACTTGAGAGTCGAAGCCAAGGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001035232
• Insert Size: 1539 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001035232.1, NP_001030309.1
• RefSeq Size: 2303 bp
• RefSeq ORF: 1539 bp
• Locus ID: 29723
• Cytogenetics: 20p12
• Gene Summary: mouse homolog facilitates transport of methotrexate and folic acid [RGD, Feb 2006]
  Transcript Variant: This variant (2) uses a different segment for its 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.