Ago1 (NM_001191765) Rat Untagged Clone

CAT#: RN217268

Ago1 (untagged) - Rat argonaute RISC catalytic component 1 (Ago1)


  "NM_001191765" in other vectors (1)

Reconstitution Protocol

USD 870.00

5 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Symbol Ago1
Synonyms Eif2c1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN217268 representing NM_001191765
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGCGGGACCCTCGGGAGCAGCTGCAGGTGCCTACCTGCCTCCCCTGCAGCAGGTGTTCCAGGCCC
CTCGCCGGCCTGGCATCGGCACTGTGGGCAAACCGATCAAGCTTCTGGCCAATTACTTTGAGGTGGACAT
TCCTAAGATTGACGTCTACCATTACGAGGTGGACATCAAGCCGGATAAGTGTCCTCGCAGAGTCAACCGG
GAGGTGGTGGAGTACATGGTCCAGCATTTCAAACCGCAGATCTTTGGGGATCGCAAGCCTGTGTACGATG
GAAAGAAGAACATTTACACAGTTACAGCACTGCCTATTGGCAATGAGAGGGTTGACTTTGAGGTGACCAT
CCCTGGGGAAGGGAAGGATAGAATTTTTAAGGTCTCCATCAAGTGGCTAGCCATCGTGAGCTGGCGCATG
CTGCATGAAGCCTTGGTCAGTGGCCAGATCCCTGTGCCCTTGGAGTCCGTGCAAGCCCTGGATGTGGCCA
TGAGGCATCTGGCATCTATGAGGTACACCCCTGTGGGCCGCTCCTTCTTCTCACCGCCTGAGGGCTACTA
CCACCCGCTGGGGGGTGGGCGCGAGGTCTGGTTCGGCTTTCACCAGTCTGTGCGCCCTGCCATGTGGAAG
ATGATGCTCAACATTGATGTCTCAGCCACTGCCTTCTACAAAGCACAGCCAGTGATTGAGTTCATGTGTG
AGGTCCTGGACATCAGGAACATAGATGAACAGCCCAAGCCCCTCACGGATTCCCAGCGCGTTCGGTTTAC
CAAGGAGATAAAGGGCCTGAAGGTGGAAGTGACCCACTGTGGACAGATGAAGAGGAAATACCGCGTGTGT
AATGTTACCCGTCGCCCTGCTAGCCATCAGACGTTTCCCTTGCAGCTGGAGAGCGGACAGACCGTGGAGT
GCACCGTGGCACAATATTTCAAGCAGAAATACAACCTTCAGCTCAAGTATCCTCACCTGCCCTGCCTGCA
AGTTGGGCAAGAGCAGAAGCATACCTATTTGCCCCTCGAGGTCTGTAACATTGTGGCTGGGCAGCGGTGC
ATTAAGAAGCTGACTGACAACCAGACTTCAACCATGATAAAGGCTACAGCTAGGTCGGCCCCAGACAGAC
AGGAGGAGATCAGTCGCCTGATGAAGAACGCCAGCTACAACTTGGATCCCTACATCCAGGAATTTGGAAT
CAAAGTGAAGGATGACATGACGGAGGTGACGGGGCGAGTGCTGCCGGCGCCCATCTTGCAGTACGGCGGC
CGGAACCGGGCCATTGCTACACCCAACCAAGGTGTCTGGGACATGCGTGGGAAACAGTTCTACAATGGGA
TTGAGATCAAAGTCTGGGCCATCGCCTGCTTCGCACCCCAAAAACAATGTCGAGAAGAGGTGCTCAAGAA
CTTCACGGACCAGCTTCGGAAGATCTCCAAGGATGCAGGGATGCCCATCCAGGGTCAGCCATGTTTCTGC
AAATACGCACAGGGGGCGGACAGCGTGGAGCCCATGTTCCGGCATCTCAAGAATACCTACTCAGGACTGC
AGCTCATTATTGTCATCCTGCCCGGGAAGACGCCGGTGTATGCTGAAGTGAAGCGTGTTGGAGATACGCT
CTTGGGAATGGCGACACAGTGTGTGCAGGTGAAGAATGTGGTCAAGACCTCACCTCAGACTCTGTCCAAC
CTCTGCCTCAAGATCAATGTCAAACTCGGTGGCATCAACAACATCCTTGTCCCACACCAGCGCTCTGCTG
TTTTCCAACAGCCAGTGATTTTTCTGGGAGCAGACGTTACACACCCCCCGGCTGGGGACGGGAAGAAACC
ATCTATCACAGCAGTGGTAGGCAGTATGGATGCACACCCCAGCCGATACTGTGCCACTGTGCGTGTACAG
CGTCCACGGCAGGAGATCATTGAGGACTTGTCCTATATGGTGCGGGAGCTGCTCATCCAGTTCTACAAGT
CCACCCGATTCAAGCCCACCCGAATCATCTTCTACCGAGATGGGGTCCCTGAAGGCCAGCTGCCTCAGAT
CCTTCACTATGAGCTGCTTGCCATTCGAGATGCCTGCATCAAACTGGAAAGAGACTACCAGCCTGGGATC
ACGTACATCGTGGTACAGAAGCGACATCACACCCGCCTCTTTTGTGCTGACAAGAATGAGCGGATTGGGA
AGAGTGGTAACATCCCAGCTGGGACCACTGTGGACACCAACATCACTCACCCGTTTGAGTTTGACTTCTA
CCTGTGCAGCCATGCAGGCATCCAGGGCACCAGCCGACCATCCCATTATTATGTCCTTTGGGATGACAAC
CGTTTCACAGCAGATGAGCTCCAGATCTTGACATACCAGCTGTGCCACACTTATGTACGATGCACACGTT
CTGTCTCTATCCCAGCACCTGCCTACTATGCCCGCCTGGTGGCTTTCCGGGCCCGATACCACCTGGTGGA
CAAGGAGCATGACAGTGGAGAGGGGAGCCACATATCTGGGCAGAGCAATGGGCGAGACCCCCAGGCCCTG
GCCAAAGCCGTGCAGGTTCACCAGGATACTCTACGCACCATGTACTTCGCTTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001191765
ORF Size 2574 bp
Insert Size 2574
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This clone expresses the complete ORF with c-terminal tags of Myc-DDK.
Reference Data
RefSeq NM_001191765.2, NP_001178694.1
RefSeq Size 7378
RefSeq ORF 2574
Locus ID 313594
Gene Summary This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to a C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015]
Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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