Ago1 (NM_001191765) Rat Untagged Clone

CAT#: RN217268

Ago1 (untagged) - Rat argonaute RISC catalytic component 1 (Ago1)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Ago1
• Synonyms: Eif2c1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217268 representing NM_001191765
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGCGGGACCCTCGGGAGCAGCTGCAGGTGCCTACCTGCCTCCCCTGCAGCAGGTGTTCCAGGCCC
  CTCGCCGGCCTGGCATCGGCACTGTGGGCAAACCGATCAAGCTTCTGGCCAATTACTTTGAGGTGGACAT
  TCCTAAGATTGACGTCTACCATTACGAGGTGGACATCAAGCCGGATAAGTGTCCTCGCAGAGTCAACCGG
  GAGGTGGTGGAGTACATGGTCCAGCATTTCAAACCGCAGATCTTTGGGGATCGCAAGCCTGTGTACGATG
  GAAAGAAGAACATTTACACAGTTACAGCACTGCCTATTGGCAATGAGAGGGTTGACTTTGAGGTGACCAT
  CCCTGGGGAAGGGAAGGATAGAATTTTTAAGGTCTCCATCAAGTGGCTAGCCATCGTGAGCTGGCGCATG
  CTGCATGAAGCCTTGGTCAGTGGCCAGATCCCTGTGCCCTTGGAGTCCGTGCAAGCCCTGGATGTGGCCA
  TGAGGCATCTGGCATCTATGAGGTACACCCCTGTGGGCCGCTCCTTCTTCTCACCGCCTGAGGGCTACTA
  CCACCCGCTGGGGGGTGGGCGCGAGGTCTGGTTCGGCTTTCACCAGTCTGTGCGCCCTGCCATGTGGAAG
  ATGATGCTCAACATTGATGTCTCAGCCACTGCCTTCTACAAAGCACAGCCAGTGATTGAGTTCATGTGTG
  AGGTCCTGGACATCAGGAACATAGATGAACAGCCCAAGCCCCTCACGGATTCCCAGCGCGTTCGGTTTAC
  CAAGGAGATAAAGGGCCTGAAGGTGGAAGTGACCCACTGTGGACAGATGAAGAGGAAATACCGCGTGTGT
  AATGTTACCCGTCGCCCTGCTAGCCATCAGACGTTTCCCTTGCAGCTGGAGAGCGGACAGACCGTGGAGT
  GCACCGTGGCACAATATTTCAAGCAGAAATACAACCTTCAGCTCAAGTATCCTCACCTGCCCTGCCTGCA
  AGTTGGGCAAGAGCAGAAGCATACCTATTTGCCCCTCGAGGTCTGTAACATTGTGGCTGGGCAGCGGTGC
  ATTAAGAAGCTGACTGACAACCAGACTTCAACCATGATAAAGGCTACAGCTAGGTCGGCCCCAGACAGAC
  AGGAGGAGATCAGTCGCCTGATGAAGAACGCCAGCTACAACTTGGATCCCTACATCCAGGAATTTGGAAT
  CAAAGTGAAGGATGACATGACGGAGGTGACGGGGCGAGTGCTGCCGGCGCCCATCTTGCAGTACGGCGGC
  CGGAACCGGGCCATTGCTACACCCAACCAAGGTGTCTGGGACATGCGTGGGAAACAGTTCTACAATGGGA
  TTGAGATCAAAGTCTGGGCCATCGCCTGCTTCGCACCCCAAAAACAATGTCGAGAAGAGGTGCTCAAGAA
  CTTCACGGACCAGCTTCGGAAGATCTCCAAGGATGCAGGGATGCCCATCCAGGGTCAGCCATGTTTCTGC
  AAATACGCACAGGGGGCGGACAGCGTGGAGCCCATGTTCCGGCATCTCAAGAATACCTACTCAGGACTGC
  AGCTCATTATTGTCATCCTGCCCGGGAAGACGCCGGTGTATGCTGAAGTGAAGCGTGTTGGAGATACGCT
  CTTGGGAATGGCGACACAGTGTGTGCAGGTGAAGAATGTGGTCAAGACCTCACCTCAGACTCTGTCCAAC
  CTCTGCCTCAAGATCAATGTCAAACTCGGTGGCATCAACAACATCCTTGTCCCACACCAGCGCTCTGCTG
  TTTTCCAACAGCCAGTGATTTTTCTGGGAGCAGACGTTACACACCCCCCGGCTGGGGACGGGAAGAAACC
  ATCTATCACAGCAGTGGTAGGCAGTATGGATGCACACCCCAGCCGATACTGTGCCACTGTGCGTGTACAG
  CGTCCACGGCAGGAGATCATTGAGGACTTGTCCTATATGGTGCGGGAGCTGCTCATCCAGTTCTACAAGT
  CCACCCGATTCAAGCCCACCCGAATCATCTTCTACCGAGATGGGGTCCCTGAAGGCCAGCTGCCTCAGAT
  CCTTCACTATGAGCTGCTTGCCATTCGAGATGCCTGCATCAAACTGGAAAGAGACTACCAGCCTGGGATC
  ACGTACATCGTGGTACAGAAGCGACATCACACCCGCCTCTTTTGTGCTGACAAGAATGAGCGGATTGGGA
  AGAGTGGTAACATCCCAGCTGGGACCACTGTGGACACCAACATCACTCACCCGTTTGAGTTTGACTTCTA
  CCTGTGCAGCCATGCAGGCATCCAGGGCACCAGCCGACCATCCCATTATTATGTCCTTTGGGATGACAAC
  CGTTTCACAGCAGATGAGCTCCAGATCTTGACATACCAGCTGTGCCACACTTATGTACGATGCACACGTT
  CTGTCTCTATCCCAGCACCTGCCTACTATGCCCGCCTGGTGGCTTTCCGGGCCCGATACCACCTGGTGGA
  CAAGGAGCATGACAGTGGAGAGGGGAGCCACATATCTGGGCAGAGCAATGGGCGAGACCCCCAGGCCCTG
  GCCAAAGCCGTGCAGGTTCACCAGGATACTCTACGCACCATGTACTTCGCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001191765
• ORF Size: 2574 bp
• Insert Size: 2574
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001191765.2, NP_001178694.1
• RefSeq Size: 7378
• RefSeq ORF: 2574
• Locus ID: 313594
• Gene Summary: This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to a C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015]
  Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.