Nfasc (NM_001160315) Rat Untagged Clone

CAT#: RN217514

Nfasc (untagged) - Rat neurofascin (Nfasc), transcript variant 4

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Nfasc
• Synonyms: NF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217514 representing NM_001160315
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGGCAGCAGGCGCCACCCTGGGTCCACGTAGCCCTCATCCTCTTCCTCCTCAGCCTCGGAGGGG
  CCATTGAGATTCCGATGGATCTGACCCAACCCCCAACGATCACCAAGCAGTCGGTGAAGGACCACATCGT
  GGACCCCCGAGATAACATCCTGATTGAATGTGAAGCTAAAGGGAACCCCGCCCCCAGTTTCCACTGGACT
  CGCAACAGCAGGTTCTTCAACATTGCCAAGGACCCACGGGTGTCCATGAGGAGGAGGTCTGGGACCTTGG
  TGATCGACTTCCGCAGTGGTGGGCGGCCTGAGGAGTACGAAGGGGAGTACCAGTGCTTTGCCCGGAACAA
  ATTCGGCACAGCTCTTAGCAACCGCATCCGCCTGCAGGTGTCCAAATCTCCCCTGTGGCCCAAGGAAAAC
  CTAGACCCCGTCGTGGTTCAAGAGGGTGCCCCCTTAACCCTGCAGTGCAACCCCCCACCTGGCCTCCCAT
  CCCCCGTCATCTTCTGGATGAGCAGCTCCATGGAGCCCATCACCCAGGACAAGCGTGTCTCCCAGGGTCA
  CAACGGGGACCTGTACTTCTCCAACGTCATGCTGCAGGACATGCAGACCGACTACAGCTGCAATGCACGC
  TTCCACTTCACCCACACCATTCAGCAGAAGAATCCCTTCACCCTCAAAGTCCTCACCACCCGAGGAGTTG
  CGGAAAGAACACCTAGCTTCATGTATCCCCAGGGCACGTCAAGCAGTCAGATGGTACTGCGCGGCATGGA
  CCTGCTGCTGGAGTGCATTGCCTCTGGGGTCCCAACACCAGATATTGCATGGTACAAGAAAGGTGGGGAC
  CTCCCATCTGACAAGGCCAAGTTCGAGAATTTTAACAAGGCTCTGCGCATCACCAATGTCTCTGAAGAGG
  ACTCTGGGGAGTATTTCTGCCTGGCCTCCAACAAGATGGGCAGCATCCGGCACACGATCTCGGTGAGAGT
  AAAGGCTGCCCCATACTGGCTGGATGAGCCCAAGAACTTGATCCTGGCTCCTGGTGAAGATGGGAGGCTG
  GTGTGTCGAGCCAATGGGAACCCGAAGCCGACCGTCCAGTGGCTGGTGAATGGAGACCCTTTGCAATCGG
  CACCACCCAACCCCAACCGTGAGGTGGCCGGAGACACTATCATCTTCCGGGACACTCAGATCAGCAGCAG
  GGCAGTGTACCAGTGTAACACATCCAACGAACATGGCTACCTGCTGGCCAATGCCTTCGTCAGCGTATTA
  GATGTACCCCCTCGGATGCTGTCTCCCCGGAACCAGCTCATCAGGGTGATCCTTTACAACCGGACGCGAC
  TGGACTGTCCGTTCTTTGGGTCTCCCATTCCAACACTCCGATGGTTTAAGAATGGGCAAGGAAGCAACCT
  GGATGGTGGTAACTACCACGTCTACGAAAATGGCAGCCTGGAAATCAAGATGATTCGCAAAGAGGACCAA
  GGCATCTACACCTGTGTGGCCACCAACATCCTGGGAAAAGCTGAAAATCAAGTCCGCCTGGAGGTCAAAG
  ACCCCACCAGGATCTACAGGATGCCTGAAGACCAGGTGGCCAAGAGGGGCACCACAGTGCAGCTGGAGTG
  CCGTGTGAAGCATGACCCCTCCTTGAAACTCACAGTCTCCTGGCTGAAGGATGACGAGCCACTCTACATT
  GGAAACAGGATGAAAAAGGAAGATGACTCCCTGACCATCTTCGGAGTGGCAGAGCGGGACCAAGGCAGTT
  ACACGTGCATGGCCAGCACCGAGCTGGACCAGGACCTGGCAAAGGCCTACCTCACTGTTCTAGGGCGGCC
  AGACCGACCCAGGGACCTGGAGCTCACTGACCTGGCGGAAAGGAGTGTGAGGCTGACCTGGATCCCGGGG
  GATGATAACAACAGTCCCATCACAGACTACGTTGTCCAGTTTGAAGAGGACCAGTTCCAGCCAGGAGTCT
  GGCATGACCACTCCAAGTTCCCAGGCAGTGTCAACTCAGCCGTCCTCCATCTGTCCCCGTATGTCAACTA
  TCAGTTCCGAGTCATCGCTGTCAACGAGGTTGGGAGCAGCCACCCCAGCCTTCCATCCGAGCGGTACCGA
  ACAAGCGGGGCACCCCCTGAATCCAACCCCAGTGATGTGAAGGGCGAAGGGACACGAAAAAACAATATGG
  AGATCACATGGACGCCTATGAATGCTACCTCCGCCTTTGGCCCCAACCTGCGCTACATTGTCAAGTGGCG
  ACGGAGAGAAACCCGAGAGACTTGGAACAATGTCACCGTGTGGGGCTCTCGCTATGTGGTGGGGCAGACC
  CCTGTCTACGTACCCTATGAGATCCGAGTCCAGGCTGAAAATGACTTTGGGAAAGGCCCGGAGCCTGAAA
  CCGTCATTGGGTACTCGGGGGAAGATTATCCCAGGGCTGCACCCACTGAAGTTAAAATCCGAGTCCTGAA
  CAGCACAGCCATCAGCCTTCAGTGGAACCGCGTCTACTCTGACACGGTCCAGGGCCAGCTCAGAGAGTAT
  CGAGCTTACTACTGGAGGGAAAGCAGTTTGCTGAAGAACCTGTGGGTGTCTCAGAAGAGACAGCAGGCCA
  GCTTCCCTGGCGACCGCCCCCGGGGCGTGGTGGGTCGCCTGTTCCCCTACAGTAACTACAAGCTGGAGAT
  GGTTGTGGTCAATGGGAGAGGTGATGGGCCTCGAAGTGAAACCAAGGAATTCACCACCCCGGAAGGAGTA
  CCCAGTGCCCCCAGGCGTTTCAGAGTCCGACAGCCCAACCTGGAGACCATCAACCTGGAATGGGATCACC
  CAGAGCACCCCAACGGGATCCTGATTGGATACACGCTCAGATACGTGCCCTTTAATGGAACCAAACTGGG
  AAAGCAGATGGTGGAAAACTTCTCTCCCAATCAGACCAAGTTCTCGGTGCAGAGAGCAGACCCCGTGTCC
  CGTTACCGCTTCTCCCTCAGTGCCAGGACGCAGGTGGGCTCTGGAGAAGCAGCCACGGAGGAGTCCCCAA
  CACCTCCAAATGAAGCTACTCCAACTGCAGCTTACACCAACAACCAGACTGACATCGCCACCCAGGGCTG
  GTTCATCGGGCTCATGTGTGCCATTGCCCTTCTGGTGCTGATCCTGCTTATCGTCTGCTTCATCAAGAGG
  AGTCGTGGCGGCAAGTACCCAGTGCGAGAAAAGAAGGATGTCCCCTTGGGCCCTGAAGACCCCAAAGAAG
  AAGATGGTTCATTTGACTACAGTGACGAGGACAACAAGCCCCTGCAGGGCAGCCAGACATCTCTGGACGG
  CACCATCAAGCAGCAGGAGAGTGACGACAGCCTAGTGGACTACGGCGAGGGTGGCGAGGGCCAGTTCAAC
  GAAGATGGCTCCTTTATTGGCCAGTACACTGTCAGAAAGGACAAGGAGGAGACCGAGGGCAATGAGAGCT
  CAGAGGCCACATCTCCAGTCAATGCCATCTATTCCCTGGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001160315
• ORF Size: 3474 bp
• Insert Size: 3474
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.

Reference Data

• RefSeq: NM_001160315.1, NP_001153787.1
• RefSeq Size: 9785
• RefSeq ORF: 3474
• Locus ID: 116690
• Gene Summary: This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined. [provided by RefSeq, May 2009]
  Transcript Variant: This variant (4) represents use of an alternate promoter and 5' UTR, and has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The resulting isoform (4) includes the third fibronectin type 3 (FNIII) repeat, lacks the mucin-like domain, and lacks two segments in the N-terminal and central regions, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.