Elf2 (NM_001033909) Rat Tagged ORF Clone

CAT#: RR200063

Elf2 (Myc-DDK-tagged ORF) - Rat E74-like factor 2 (Elf2), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Elf2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200063 representing NM_001033909
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCAGCAGTGGTTGGCGGCGGAGGCTCTGTTCTGGAGCTTTCCAGCAATGGAGTAGAAAATCAAG
  AGGGAGGGGACACGGTCCCTGAATTTCCGGCAGTGATTGTGGAGCCCGTCCCCAGTGCGAGGTTAGAGCA
  GGGCTACGCTGCCCAGGTGCTGGTGTATGACGATGAGACTTACATGATGCAGGATGTGGCGGAAGAACAA
  GAAGTTGAGACGGAGAATTCAGAAACAGTGGAAGCATCAGTTCACAGCAGTAATGCACACTGTACGGATA
  AGACCATTGAAGCTGCTGAAGCCCTGCTTCATATGGAGTCTCCTACCTGCTTGAGGGATTCAAGAAGTCC
  CGTGGAAGTGTTTGTCCCTCCTTGTATATCAACTCCAGAGTTTATCCATGCTGCGATGAGGCCAGATGTC
  ATTACGGAGACTGTAGTGGAGGTGTCAACAGAGGAGTCTGAACCAATGGATGCCTCGCCTATCCCTACTT
  CACCAGACAGCCACGAACCAATGAAAAAGAAGAAAGTTGGCCGTAAACCAAAGACCCAGCAGTCACCAGT
  TTCCAATGGGTCTCCTGAGTTAGGAGTCAAGAAGAAAGCCAGAGAAGGAAAAGGAAACACAACCTATCTG
  TGGGAGTTTCTTTTAGATCTGCTTCAAGATAAAAATACTTGTCCCAGGTATATTAAATGGACTCAGAGAG
  AAAAGGGCATATTCAAGCTGGTGGACTCAAAGGCTGTCTCGAAGCTCTGGGGAAAGCATAAGAACAAACC
  AGACATGAACTATGAAACTATGGGGCGAGCTCTGAGATACTACTACCAAAGGGGAATTCTTGCAAAGGTT
  GAAGGACAGAGGCTCGTATATCAGTTCAAGGACATGCCCAAAAACATAGTGGTCATTGATGATGACAAAA
  GTGAGACTTGCCCGGAAGACTTGGCAGCAGCTGCTGATGACAAGTCTTTAGAACGAGTGTCACTATCTGC
  AGAAAGTCTCCTGAAGGCAGCGACTGCCGTCCGAGGTGGGAAAAACTCATCTCCTATGAACTGCTCCAGA
  GCAGAGAAGGGTGTGACTAGAGTTGTGAACATCACTTCCCCTGCTCACGATGCTTCATCCAGGTGTCCTA
  CCACCACAGCACCCGTGTCAGCAGCAGCAGCTCCAAGGACAGTTCGTGTGGCAATGCAAGTCCCTGTGGT
  AATGACATCACTGGGTCAAAAGATTTCCACTGTGGCAGTTCAGTCAGTCAATGCAGGCACACCATTAATA
  ACCAGCACCAGTCCAGCTACAGCCAGCTCTCCAAAAGTAGTCATCCAGACGGTCCCGACTGTGATGCCAG
  CCTCCGCTGAGAATGGAGACAGAATCACCATGCAGCCTGCCAAGATTATCACCATCCCCGCTACCCAGCT
  TGCACAGTGTCAGCTGCAGACAAAGTCAAATCTGACTGGGTCAGGAAGCATTAGCCTTGTTGGAACCCCC
  CTGGCTGTGAGAGCGCTCACCCCTGTTTCAATAGCCCACGGTACACCTGTGATGAGACTATCTGTGCCCG
  CTCAGCAGGCTTCTGGCCAGACTCCTCCTCGAGTTATCAGTGCGGTCATAAAAGGGCCAGAGGTTAAATC
  AGAAGCCAAAAAGCAGGAACACGATGTGAAAACTTTGCAGCTGGTAGAAGAAAAGGGGGCAGACGGAAAT
  AAGACAGTAACCCATGTAGTGGTTGTCAGTGCGCCCTCTGCTATCGCCCTTCCTGTGACTATGAAAACCG
  AAGGGCTGGTGACGTGTGAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200063 representing NM_001033909
  Red=Cloning site Green=Tags(s)
  
  MASAVVGGGGSVLELSSNGVENQEGGDTVPEFPAVIVEPVPSARLEQGYAAQVLVYDDETYMMQDVAEEQ
  EVETENSETVEASVHSSNAHCTDKTIEAAEALLHMESPTCLRDSRSPVEVFVPPCISTPEFIHAAMRPDV
  ITETVVEVSTEESEPMDASPIPTSPDSHEPMKKKKVGRKPKTQQSPVSNGSPELGVKKKAREGKGNTTYL
  WEFLLDLLQDKNTCPRYIKWTQREKGIFKLVDSKAVSKLWGKHKNKPDMNYETMGRALRYYYQRGILAKV
  EGQRLVYQFKDMPKNIVVIDDDKSETCPEDLAAAADDKSLERVSLSAESLLKAATAVRGGKNSSPMNCSR
  AEKGVTRVVNITSPAHDASSRCPTTTAPVSAAAAPRTVRVAMQVPVVMTSLGQKISTVAVQSVNAGTPLI
  TSTSPATASSPKVVIQTVPTVMPASAENGDRITMQPAKIITIPATQLAQCQLQTKSNLTGSGSISLVGTP
  LAVRALTPVSIAHGTPVMRLSVPAQQASGQTPPRVISAVIKGPEVKSEAKKQEHDVKTLQLVEEKGADGN
  KTVTHVVVVSAPSAIALPVTMKTEGLVTCEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033909
• ORF Size: 1773 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033909.1, NP_001029081.1
• RefSeq Size: 3199 bp
• RefSeq ORF: 1776 bp
• Locus ID: 361944
• Cytogenetics: 2q26
• MW: 63.2 kDa