Elf2 (NM_001012181) Rat Tagged ORF Clone

CAT#: RR200064

Elf2 (Myc-DDK-tagged ORF) - Rat E74-like factor 2 (Elf2), transcript variant 2, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Elf2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200064 representing NM_001012181
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACGTCTCTGCATGAGGGACCCACGAACCAGCTGGATCTGCTCATCCGGGCCGTGGAAGCATCAG
  TTCACAGCAGTAATGCACACTGTACGGATAAGACCATTGAAGCTGCTGAAGCCCTGCTTCATATGGAGTC
  TCCTACCTGCTTGAGGGATTCAAGAAGTCCCGAGTTTATCCATGCTGCGATGAGGCCAGATGTCATTACG
  GAGACTGTAGTGGAGGTGTCAACAGAGGAGTCTGAACCAATGGATGCCTCGCCTATCCCTACTTCACCAG
  ACAGCCACGAACCAATGAAAAAGAAGAAAGTTGGCCGTAAACCAAAGACCCAGCAGTCACCAGTTTCCAA
  TGGGTCTCCTGAGTTAGGAGTCAAGAAGAAAGCCAGAGAAGGAAAAGGAAACACAACCTATCTGTGGGAG
  TTTCTTTTAGATCTGCTTCAAGATAAAAATACTTGTCCCAGGTATATTAAATGGACTCAGAGAGAAAAGG
  GCATATTCAAGCTGGTGGACTCAAAGGCTGTCTCGAAGCTCTGGGGAAAGCATAAGAACAAACCAGACAT
  GAACTATGAAACTATGGGGCGAGCTCTGAGATACTACTACCAAAGGGGAATTCTTGCAAAGGTTGAAGGA
  CAGAGGCTCGTATATCAGTTCAAGGACATGCCCAAAAACATAGTGGTCATTGATGATGACAAAAGTGAGA
  CTTGCCCGGAAGACTTGGCAGCAGCTGCTGATGACAAGTCTTTAGAACGAGTGTCACTATCTGCAGAAAG
  TCTCCTGAAGGCAGCGACTGCCGTCCGAGGTGGGAAAAACTCATCTCCTATGAACTGCTCCAGAGCAGAG
  AAGGGTGTGACTAGAGTTGTGAACATCACTTCCCCTGCTCACGATGCTTCATCCAGGTGTCCTACCACCA
  CAGCACCCGTGTCAGCAGCAGCAGCTCCAAGGACAGTTCGTGTGGCAATGCAAGTCCCTGTGGTAATGAC
  ATCACTGGGTCAAAAGATTTCCACTGTGGCAGTTCAGTCAGTCAATGCAGGCACACCATTAATAACCAGC
  ACCAGTCCAGCTACAGCCAGCTCTCCAAAAGTAGTCATCCAGACGGTCCCGACTGTGATGCCAGCCTCCG
  CTGAGAATGGAGACAGAATCACCATGCAGCCTGCCAAGATTATCACCATCCCCGCTACCCAGCTTGCACA
  GTGTCAGCTGCAGACAAAGTCAAATCTGACTGGGTCAGGAAGCATTAGCCTTGTTGGAACCCCCCTGGCT
  GTGAGAGCGCTCACCCCTGTTTCAATAGCCCACGGTACACCTGTGATGAGACTATCTGTGCCCGCTCAGC
  AGGCTTCTGGCCAGACTCCTCCTCGAGTTATCAGTGCGGTCATAAAAGGGCCAGAGGTTAAATCAGAAGC
  CAAAAAGCAGGAACACGATGTGAAAACTTTGCAGCTGGTAGAAGAAAAGGGGGCAGACGGAAATAAGACA
  GTAACCCATGTAGTGGTTGTCAGTGCGCCCTCTGCTATCGCCCTTCCTGTGACTATGAAAACCGAAGGGC
  TGGTGACGTGTGAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200064 representing NM_001012181
  Red=Cloning site Green=Tags(s)
  
  MATSLHEGPTNQLDLLIRAVEASVHSSNAHCTDKTIEAAEALLHMESPTCLRDSRSPEFIHAAMRPDVIT
  ETVVEVSTEESEPMDASPIPTSPDSHEPMKKKKVGRKPKTQQSPVSNGSPELGVKKKAREGKGNTTYLWE
  FLLDLLQDKNTCPRYIKWTQREKGIFKLVDSKAVSKLWGKHKNKPDMNYETMGRALRYYYQRGILAKVEG
  QRLVYQFKDMPKNIVVIDDDKSETCPEDLAAAADDKSLERVSLSAESLLKAATAVRGGKNSSPMNCSRAE
  KGVTRVVNITSPAHDASSRCPTTTAPVSAAAAPRTVRVAMQVPVVMTSLGQKISTVAVQSVNAGTPLITS
  TSPATASSPKVVIQTVPTVMPASAENGDRITMQPAKIITIPATQLAQCQLQTKSNLTGSGSISLVGTPLA
  VRALTPVSIAHGTPVMRLSVPAQQASGQTPPRVISAVIKGPEVKSEAKKQEHDVKTLQLVEEKGADGNKT
  VTHVVVVSAPSAIALPVTMKTEGLVTCEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012181
• ORF Size: 1557 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012181.1, NM_001012181.2, NP_001012181.1
• RefSeq Size: 3519 bp
• RefSeq ORF: 1560 bp
• Locus ID: 361944
• Cytogenetics: 2q26
• MW: 55.7 kDa