Zc3h14 (NM_001033951) Rat Tagged ORF Clone

CAT#: RR200172

Zc3h14 (Myc-DDK-tagged ORF) - Rat zinc finger CCCH type containing 14 (Zc3h14), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zc3h14
• Synonyms: Npuk68
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200172 representing NM_001033951
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAATCGGCACCGAGATCAGCCGCAAGATCCGGAGTGCCATTAAGGGGAAATTACAAGAATTAGGAG
  CTTACGTGGATGAAGAACTTCCTGATTACATTATGGTGATGGTGGCCAACAAGAAAAGTCAGGACCAAAT
  GACAGAGGACCTGTCCCTGTTTCTAGGGAACAACACAATTCGATTCACCGTATGGCTCCATGGTGTATTA
  GATAAACTTCGTTCTGTTACAACTGAGCCCTCTAGTCTAAAGTCTCCTGATACCAGCATCTTTGATAGTA
  ACGTGCCTTCAAACAAGAGCAGTTTTAGTCGGGGAGATGAGAGAAGGCATGAAGCTGCCATTCCTCCCCT
  TGCCGTTTCTAGCTCTAGACCTGAGAAAAGGGATTCCAGAGTTTCTACAAGTTCACAGGAGCACAAATCC
  ACTAACGTCAGACATTCATATGATGATGGAGCTTCCACCAGGCTAATGTCAACAGTGAAACCTTTGAGGG
  AGCCAGCACCCTCTGAAGATGTGATTGATATCAAGCCAGAACCAGATGATCTCATTGATGAAGACCTCAA
  TTTTGTGCAGGAGAATTCCTTATCTCAGAAAAAACCTACAGTGACACTTACATACGGTTCTTCTCGCCCT
  TCTATAGAAATTTATCGACCACCTGCAAGTAGAAATGCAGACACTGGTACTCATTTAAACAGGCCGCAAC
  TTCAGCAGCAGCAAAGCAGTACCCATACTGCCAAGCAGCTGGATGGACAGAGCAGCCAGGTGTATGAAGC
  AGGACGGTTGTGTGAACCAGAAGTACTTGGCAGCGTAGAAGATACTTATAGCCCTTTCTTCAGAAACAAC
  TTGGATAAAATGAATATTGAGGAGGAAAACTTTCGGAAGAGAAAGTTGCCTGTGGTAAGTTCAGTTGTTA
  AAGTAAAAAGATTTAGCCATGATGGAGAAGAGGAGGAAGAAGATGAGGATTATGGGACCCGGGTAGGAAG
  CTTGTCCAGCAGTGTGTCAGTACCAGCAAAACCTGAGAGGAGACCTTCTCTTCCACCTTCTAAACAAGCT
  AACAAGAATCTAATTTTGAAGGCTATCTCTGAAGCTCAAGAGTCTGTAACAAAAACAACTAACTATCCTG
  CAGTTCCACAGAAACAGACACTTCCAGTTGCTCCTAGAACTCGAACTTCTCAAGAAGAAGTGCTCGCAGA
  AATGGTCCAGGGGCAAAACAGGGCCCCCAGAATAAGCCCCCCAGTTAAAGAAGAGGAAGCAAAAGGAGAT
  AATGCAGAAAAAATTGAAGGAACTCAACAGAGGCAATTGTTATCCCGACTGCAAATTGATCCAGTAACGG
  TAGACACAATGGAGCTGAGTCAAGATTACTATGACATGGAATCCATGGTCCATGCAGACACAAGATCATT
  TATTCTGAAGAAGCCAAAGCTGTCTGAGGAAATAGTAGTGACACCAAACCAGGATTCGGGGATGAAGACT
  GCAGATGCCCTTCGGGTCCTTTCAGGACACCTTATGCAGACACGAGATCTTGTACAACCAGATAAACCTG
  CAAGTCCCAAGTTTATAGTGACGCTGGATGGTGTCCCCAGCCCCCCAGGATACATGTCAGATCAAGAGGA
  GGAGATGTGCTTTGAAGGAATGAAACCCGTAAACCAAACTTCAGCCTCAAACAAGGGACTCAGAGGCCTC
  CTCCACCCACAGCAGTTGCATTTGCTGAGCAGGCAGCTTGAGGACCCAGATGGTAGCTTTTCCAACGCCG
  AGATGACTGACCTGAGTGTGGCACAGAAACCAGAAAAACTCCTGGAGCGCTGCAAGTACTGGCCTGCTTG
  TAAAAATGGGGATGAGTGTGTGTACCATCACCCCATTTCACCTTGCAAAGCCTTTCCCAACTGTAAATTT
  GCTGAGAAATGTTTGTTTGTGCATCCAAATTGTAAATATGACGCAAAGTGTACTAAAGCAGATTGTCCCT
  TCACTCACATGAGTAGAAGAGGTCCAGTACTGACTCCAAAACCAGCAGTGTCGTCACCAGCGCCATCTTC
  TAATGGCCAGTTCTGCCGTTACTTCCCTGCCTGTAAGAAAATGGAGTGTCCCTTCTACCACCCCAAACAC
  TGTAGGTTTAATACCCAGTGTACAAGACCAGACTGTACGTTTTATCACCCCACTATTACTGTGCCACCAA
  GACATGCCTTGAAATGGATTCGACCTCAAACCAGTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200172 representing NM_001033951
  Red=Cloning site Green=Tags(s)
  
  MEIGTEISRKIRSAIKGKLQELGAYVDEELPDYIMVMVANKKSQDQMTEDLSLFLGNNTIRFTVWLHGVL
  DKLRSVTTEPSSLKSPDTSIFDSNVPSNKSSFSRGDERRHEAAIPPLAVSSSRPEKRDSRVSTSSQEHKS
  TNVRHSYDDGASTRLMSTVKPLREPAPSEDVIDIKPEPDDLIDEDLNFVQENSLSQKKPTVTLTYGSSRP
  SIEIYRPPASRNADTGTHLNRPQLQQQQSSTHTAKQLDGQSSQVYEAGRLCEPEVLGSVEDTYSPFFRNN
  LDKMNIEEENFRKRKLPVVSSVVKVKRFSHDGEEEEEDEDYGTRVGSLSSSVSVPAKPERRPSLPPSKQA
  NKNLILKAISEAQESVTKTTNYPAVPQKQTLPVAPRTRTSQEEVLAEMVQGQNRAPRISPPVKEEEAKGD
  NAEKIEGTQQRQLLSRLQIDPVTVDTMELSQDYYDMESMVHADTRSFILKKPKLSEEIVVTPNQDSGMKT
  ADALRVLSGHLMQTRDLVQPDKPASPKFIVTLDGVPSPPGYMSDQEEEMCFEGMKPVNQTSASNKGLRGL
  LHPQQLHLLSRQLEDPDGSFSNAEMTDLSVAQKPEKLLERCKYWPACKNGDECVYHHPISPCKAFPNCKF
  AEKCLFVHPNCKYDAKCTKADCPFTHMSRRGPVLTPKPAVSSPAPSSNGQFCRYFPACKKMECPFYHPKH
  CRFNTQCTRPDCTFYHPTITVPPRHALKWIRPQTSE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033951
• ORF Size: 2208 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033951.1, NP_001029123.1
• RefSeq Size: 3449 bp
• RefSeq ORF: 2211 bp
• Locus ID: 192359
• Cytogenetics: 6q32
• MW: 82.6 kDa
• Gene Summary: recognized by an antibody against a liver cell chromatin fraction [RGD, Feb 2006]