Shank2 (NM_001004133) Rat Tagged ORF Clone

CAT#: RR200193

Shank2 (Myc-DDK-tagged ORF) - Rat SH3 and multiple ankyrin repeat domains 2 (Shank2), transcript variant 2, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Shank2
• Synonyms: CortBP1; ProSAP1; Spank-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200193 representing NM_001004133
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAGCGTCCCCGGCGGTGGAGCAGCCACCGTGATGATGACCGGTTACAATAATGGTCGCTATCCCC
  GGAATTCTCTCTACAGTGACTGCATTATTGAGGACAAGACGGTGGTCCTGCAGAAGAAAGACAACGAGGG
  CTTTGGATTTGTGCTCCGAGGGGCAAAAGCTGATACCCCCATTGAGGAATTCACACCCACGCCGGCATTT
  CCAGCCCTGCAGTACCTGGAGTCCGTGGATGAAGGAGGGGTGGCATGGCAAGCCGGACTAAGGACCGGGG
  ACTTCTTGATTGAGGTTAACAATGAAAATGTCGTCAAGGTGGGCCACAGGCAGGTGGTGAACATGATCCG
  CCAGGGAGGGAATCACCTCGTCCTTAAGGTCGTCACGGTGACCAGGAATCTAGACCCTGATGATACAGCC
  AGAAAGAAAGCTCCCCCACCTCCAAAGCGGGCTCCGACCACGGCCCTCACCGTGCGTTCCAAGTCCATGA
  CAGCGGAATTGGAGGAACTCGTGGATAAAGATAAACCGGAAGAGATAGTCCCAGCCTCCAAGCCCTCCAG
  GACTGCAGAGAACGTGGCCATAGAATCCAGGGTGGCAACCATCAAGCAGCGGCCCACAAGCCGGTGCTTT
  CCAGCTGCCTCTGATGTGAACTCCGTGTACGAGCGCCAAGGGATTGCTGTAATGACGCCCACGGTCCCTG
  GGAGCCCGAAAGGCCCATTTCTGGGCCTCCCTCGAGGTACGATGCGAAGGCAGAAATCGATAGACAGCAG
  AATCTTTCTATCAGGGATAACAGAGGAAGAGCGGCAGTTTCTGGCTCCCCCAATGCTGAAGTTCACCCGA
  AGCTTGTCCATGCCAGACACTTCTGAGGACATCCCCCCTCCGCCACAGTCTGTGCCCCCCTCTCCCCCTC
  CACCTTCCCCCACCACATACAACTGTCCCAGGTCCCCGACTCCAAGAGTCTATGGGACAATTAAGCCTGC
  GTTCAACCAGAACCCCGTCGCCAAGGTGCCCCCAGCCACCAGGTCTGACACTGTGGCCACCATGATGCGG
  GAAAAGGGGATGTTCTACAGGAGAGAGCTGGACCGCTTTTCCCTGGACTCAGAAGACGTCTACAGCCGCA
  GCCCCGCCCCACAGGCTGCCTTCCGCACCAAGCGGGGACAGATGCCTGAGAACCCGTACTCGGAGGTGGG
  AAAGATAGCCAGCAAGGCCGTCTATGTCCCTGCCAAGCCAGCCAGGCGGAAGGGCATGCTGGTAAAGCAG
  TCCAACGTGGAGGACAGCCCTGAGAAGACGTGCTCCATCCCCATCCCAACCATCATAGTCAAGGAACCCT
  CCACCAGCAGCAGCGGCAAGAGCAGCCAGGGGAGCAGCATGGAGATCGACCCCCAGGCCACTGAGCCCGG
  CCAGCTGCGGCCAGATGACAGCCTCACCGTCAGCAGCCCTTTCGCGGCAGCCATCGCTGGGGCCGTGCGT
  GACCGGGAGAAGCGTCTGGAAGCCAGGAGGAATTCCCCGGCCTTCCTCTCCACCGACCTGGGAGATGAGG
  ACGTGGGTCTGGGGCCTCCTGCTCCCCGGATGCAGCCCTCCAAGTTTCCCGAGGAGGGTGGGTTTGGTGA
  TGAGGATGAAACGGAACAGCCGCTATTGCCTACCCCTGGAGCAGCGCCCAGGGAGCTGGAGAACCACTTC
  CTAGGTGGTGGTGAGGCTGGTGCTCAGGGGGAGGCTGGGGGACCCCTGAGTTCCACATCCAAAGCCAAGG
  GGCCTGAAAGTGGCCCAGCAGCCGCCCTCAAGAGCAGCAGCCCAGCCAGCCCTGAGAATTACGTGCACCC
  ACTCACGGGGCGGCTGCTCGACCCCAGCTCCCCGCTGGCCCTGGCACTGTCCGCCAGGGACCGAGCCATG
  CAGGAGTCCCAGCAAGGACACAAGGGGGAGGCCCCCAAGGCCGACCTTAACAAGCCTCTTTACATCGATA
  CCAAAATGCGGCCCAGTGTGGAGTCCGGCTTTCCACCGGTCACCAGACAGAACACCAGGGGTCCCCTGCG
  AAGGCAGGAGACAGAGAACAAGTATGAGACGGACCTGAGCAAGGACCGGAGGGCTGACGACAAGAAGAAC
  ATGCTGATCAACATCGTGGACACTGCCCAGCAGAAGTCAGCCGGCCTACTGATGGTGCACACAGTGGACA
  TTCCCGTGGCGGGGCCACCCCTGGAGGAAGAGGAGGACAGAGAGGATGGGGATACAAAGCCAGACCACTC
  ACCCTCCACAGTGCCAGAAGGCGTTCCCAAAACCGAAGGTGCTTTACAGATCTCCGCTGCCCCGGAGCCC
  GCCGCTGCGCCCGGCAGGACCATCGTGGCAGCGGGCTCCGTGGAAGAGGCGGTAATTCTGCCATTCCGCA
  TCCCCCCTCCCCCTCTGGCGTCCGTGGACTTGGATGAGGACTTTCTTTTCACAGAACCATTGCCTCCTCC
  CCTGGAATTCGCCAATAGTTTTGATATCCCCGACGACCGGGCAGCTTCAGTTCCCGCTCTGGCTGACCTG
  GTCAAGCAGAAGAAAAGTGACACCCCTCAGCCCCCTTCGTTGAACTCCAGCCAACCAGCCAACTCTACAG
  ACAGTAAGAAGCCAGCCGGCATCTCAAACTGTCTGCCCTCCTCATTCCTGCCACCCCCCGAAAGCTTTGA
  CGCGGTCACCGACTCGGGGATCGAGGAGGTGGACAGCCGGAGTAGCAGCGACCACCACCTGGAGACAACC
  AGCACCATCTCCACAGTGTCCAGCATCTCCACGCTGTCTTCAGAGGGCGGGGAGAGCATGGATACTTGCA
  CAGTCTATGCAGACGGGCAAGCCTTTGTGGTTGACAAGCCCCCAGTACCTCCAAAGCCAAAAATGAAGCC
  CATCGTTCACAAAAGCAACGCACTTTACCAAGACACGCTCCCAGAAGAGGACACAGATGGCTTTGTGATC
  CCCCCACCTGCACCCCCGCCCCCGCCCGGCAGTGCCCAGGCCGGTGTGGCGAAGGTCATCCAGCCAAGGA
  CCTCCAAGTTGTGGGGTGATGTTACAGAGGTCAAAAGCCCAATTCTCTCAGGCCCAAAGGCAAATGTCAT
  TAGTGAGTTAAACTCCATTCTGCAGCAGATGAACAGGGGGAAATCGGTCAAGCCTGGGGAAGGGCTGGAG
  CTGCCCGTGGGAGCCAAGTCAGCTAACCTCGCTCCAAGAAGCCCGGAGGTCATGAGCACTGTCTCAGGTA
  CACGGAGCACGACGGTCACCTTCACTGTCCGCCCCGGAACCTCCCAGCCCATCACCCTACAGAGCCGGCC
  CCCTGACTATGAAAGCAGGACCTCAGGACCGAGACGTGCCCCAAGCCCTGTGGTTTCGCCAACGGAATTG
  AGCAAAGAGATTCTGCCCACCCCTCCGTCTGCTGCAGCAGCCTCTCCCTCCCCCACACTCTCAGATGTCT
  TTAGCCTTCCAAGCCAGTCCCCTGCAGGGGACCTCTTTGGCTTGAACCCAGCAGGACGCAGCAGGTCACC
  ATCTCCTTCAATATTGCAACAGCCAATCTCAAATAAGCCTTTTACAACTAAGCCTGTCCACCTGTGGACG
  AAACCAGATGTGGCAGACTGGCTGGAAAGTCTGAACTTGGGTGAACACAAGGAGACGTTCATGGACAATG
  AGATTGACGGCAGCCATCTGCCAAACCTCCAGAAGGAAGACTTGATAGATCTTGGGGTGACTCGAGTTGG
  GCACAGGATGAACATAGAAAGGGCTTTGAAACAGCTGCTGGACAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200193 representing NM_001004133
  Red=Cloning site Green=Tags(s)
  
  MMSVPGGGAATVMMTGYNNGRYPRNSLYSDCIIEDKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAF
  PALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVNMIRQGGNHLVLKVVTVTRNLDPDDTA
  RKKAPPPPKRAPTTALTVRSKSMTAELEELVDKDKPEEIVPASKPSRTAENVAIESRVATIKQRPTSRCF
  PAASDVNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTR
  SLSMPDTSEDIPPPPQSVPPSPPPPSPTTYNCPRSPTPRVYGTIKPAFNQNPVAKVPPATRSDTVATMMR
  EKGMFYRRELDRFSLDSEDVYSRSPAPQAAFRTKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQ
  SNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSMEIDPQATEPGQLRPDDSLTVSSPFAAAIAGAVR
  DREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRMQPSKFPEEGGFGDEDETEQPLLPTPGAAPRELENHF
  LGGGEAGAQGEAGGPLSSTSKAKGPESGPAAALKSSSPASPENYVHPLTGRLLDPSSPLALALSARDRAM
  QESQQGHKGEAPKADLNKPLYIDTKMRPSVESGFPPVTRQNTRGPLRRQETENKYETDLSKDRRADDKKN
  MLINIVDTAQQKSAGLLMVHTVDIPVAGPPLEEEEDREDGDTKPDHSPSTVPEGVPKTEGALQISAAPEP
  AAAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFLFTEPLPPPLEFANSFDIPDDRAASVPALADL
  VKQKKSDTPQPPSLNSSQPANSTDSKKPAGISNCLPSSFLPPPESFDAVTDSGIEEVDSRSSSDHHLETT
  STISTVSSISTLSSEGGESMDTCTVYADGQAFVVDKPPVPPKPKMKPIVHKSNALYQDTLPEEDTDGFVI
  PPPAPPPPPGSAQAGVAKVIQPRTSKLWGDVTEVKSPILSGPKANVISELNSILQQMNRGKSVKPGEGLE
  LPVGAKSANLAPRSPEVMSTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRTSGPRRAPSPVVSPTEL
  SKEILPTPPSAAAASPSPTLSDVFSLPSQSPAGDLFGLNPAGRSRSPSPSILQQPISNKPFTTKPVHLWT
  KPDVADWLESLNLGEHKETFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004133
• ORF Size: 3756 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004133.1, NP_001004133.1
• RefSeq Size: 4636 bp
• RefSeq ORF: 3759 bp
• Locus ID: 171093
• Cytogenetics: 1q42
• MW: 134.3 kDa
• Gene Summary: binds to cortactin and alpha-latrotoxin receptor and is involved in the structural and functional organization of the post synaptic density [RGD, Feb 2006]