Rtn3 (NM_080909) Rat Tagged ORF Clone

CAT#: RR200211

Rtn3 (Myc-DDK-tagged ORF) - Rat reticulon 3 (Rtn3), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rtn3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200211 representing NM_080909
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGTCATCGGCGGCCACTCAGTCTCCGTCAGTCTCCTCGTCGTCCTCCGGGGCCGAGCCGTCAA
  CGCTTGGCGGCGGCGGCGGGAGCCCTGGAGCCTGCCCCGCCCTGGGGGCGAAGAGCTGCGGCTCCTCCTG
  TGCGGTGGGATTGAGCTCTCTTTGCTCTGATGAGCCACCATCAAAAATTATGACTTCCTCCTTTCTTTCA
  TCTTCTGAAATATATAACCCTGACCCTACAACACCACTTGGAAGTGAAACATTAGGCAGCCATCTTGTAG
  CTAAAGACGGAAAAGATCCCTTGGTTCTTCTAGATAAGAAAACTCTGGACTCACCTCAGGGGACCAACAA
  GGACAGAGTAGACACTCCGGTTTCTCTTGCAGCTGACATTCCTTGCAGCCACCCTTCTATTCCAGACAGT
  TTCCCAGAACAACCTACTTTTCTTTCAAAGGAAACTGATCCAACAGAAGAGTGGGTAGTTAAAAACCAAG
  AACCCAAGAACCCAAGTGAAGTTCCAAGTGGAGAGGACAGAAGTGCACTGGATCTTGGGCAGCGTAAGGC
  AGAACACATTTGTACACATTCCTTGTCTCCATCTGAACCTGCAGTAGCCAGTGTAGAAAAAGATTCTCCT
  GAGTCACCATTTGAAGTAATTATTGACAAAGCAACATTTGACAGAGAATTTAAAGATTTGTATAAGGAAA
  GCACAGATGATTTGGGTGGCTGGGCAGCTCACGGTGACAGAGAATCCCCAGCAGATTTATTGGAAATGAA
  TGACAAAGTGTTTCCACTGAGAAATAAAGAGGCAGGGCGTTACCCATCCTCTGCACTGCTCGGTAGACAA
  TTCTCACACACTACAGCAGCACTGGAAGAAGTGTCTAGATGTGTGAATGATATGCATAACTTTACTAATG
  AAATACTGACTTGGGACTTAGATCCCCAAGCAAAACAACAGGCGGACAAATCACCTTGCACCACAGAAAG
  TACAGGACTAGACAGGAGTGAACATCGCTCAGAAATTCCAGTTATAAATCTTAAAACAAGCCCTCAACAG
  AAAATGCCTGTATGTTCTTTTAATGGGAGCACTCCCATTACTAGATCCACAGGTGACTGGACAGAAACAT
  TTACAGAAGGAAAACCTGTAAAAGGCTACCTCAGCTCCACAAAAGAAGCTGGTGGCAAAGGTGTACCAGA
  CAGTTCTCAGCCTATCTCAGGCTCTGGAGCAGCCACAGTGGAAGTGACTTTACCTGACCTGAGGGGTACC
  TGGCCTAACTCTGTGCTAGGGGAAGTCACAGAGGTTGATAGTTCTGGGGAATCTGATGACACAGTAATAG
  AGGACACCACAGAAAATCCCTCAGTTAAGAACAACAAAGTTTTGCCAGAAAAATCTGACTCCCTTCCAAG
  TGCTGCTGTAAAAACAAGTGAAAGGGAAAACAAAGAGACTACCAGTCATGAAACTGTGAGGAGTGAAATG
  TATGAAAACTCTGAGCAGCAGCAGGCCCATGCAGAAACTCCTACTCAGAGGAGTCTGGAAGGTCAGATGT
  CTCCACAAGTACCTAATATGCTGAATGAAGTCATACCTGAAAATCTTGCTATGACTGACACAACCAGAGT
  TTGCTCAGCAGCACCTCCAAGTGTTCTTAGTGAGACTGGATTCTCACTAACTGTGCCAGCTTCTGCCAAG
  TCAGAATCTTTGCTTGGAAAAAATGTTGAAGATACAGATGGTTCCTCTCCAGAGGACTTGATGGCTGCCC
  TCACAGGAACTGAGGAGAAGGGGACAGTGGATAAAGAGAAAGGTGATGTTTTGGAAGCAGTGTTAGAGAA
  GATAGCAAACGTTAAAAACACTTTGCCTGTGGAACTCTTGCATGAAAATAAGCTAAGTAGTTCTGAAACC
  AAAAATACTAAAAGCAAATACAGTGAACACAGCAGAGAAACAAATGGAGGTGCCCCCAAGGTGTCTTCCG
  ACTTAGAGCAGGAGCAGCTCACCATTAGAGCCATTAAAGAATTAGGGGCTAAGCAGGTGCAGGCTGAAAG
  AATGTCTCCTGGAGGAAAACTCAAGCGAACCTTTGATCCACAGTCTGGGCCACAGAATTCATCTGACATC
  CTAGAACACACAGATATCACAACTGGATCTGATCTTGGGATTCCCAAAAAGCCTACTATCATCAAAGACA
  CTAGAATAGATTCTATTTCCAGCCTTACCAAGACTGAAACAGTTAACAAGCATGTTCTAGCAAGACTTCT
  CAGTGATTTCCCAGTGCATGATCTGATTTTCTGGCGAGATGTGAAGAAGACTGGGTTTGTCTTTGGCACC
  ACGCTGATCATGCTGCTCTCTCTGGCAGCTTTCAGTGTTATCAGTGTGGTCTCTTACCTCATCCTGGCTC
  TACTCTCTGTCACCATCAGCTTCAGAGTCTACAAGTCTGTCATCCAAGCTGTGCAGAAGTCAGAAGAAGG
  ACATCCATTCAAGGCCTACCTGGATGTGGACATTACACTGTCCTCAGAAGCTTTCCACAGCTACATGAAT
  GCTGCAATGGTGCATGTCAACAAGGCCCTCAAACTCATTATTCGTCTCTTTCTGGTAGAAGACTTGGTTG
  ACTCCTTGAAGCTGGCTGTCTTCATGTGGCTGATGACCTACGTCGGTGCTGTTTTTAACGGAATTACCCT
  TCTGATTCTCGCCGAGCTGCTGGTTTTCAGCGTCCCAATTGTCTATGAGAAGTATAAGACACAGATTGAC
  CACTATGTTGGGATTGCCCGGGATCAGACCAAGTCAATTGTTGAAAAGATCCAAGCAAAGCTTCCTGGAA
  TCGCCAAAAAAAAGGCAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200211 representing NM_080909
  Red=Cloning site Green=Tags(s)
  
  MAESSAATQSPSVSSSSSGAEPSTLGGGGGSPGACPALGAKSCGSSCAVGLSSLCSDEPPSKIMTSSFLS
  SSEIYNPDPTTPLGSETLGSHLVAKDGKDPLVLLDKKTLDSPQGTNKDRVDTPVSLAADIPCSHPSIPDS
  FPEQPTFLSKETDPTEEWVVKNQEPKNPSEVPSGEDRSALDLGQRKAEHICTHSLSPSEPAVASVEKDSP
  ESPFEVIIDKATFDREFKDLYKESTDDLGGWAAHGDRESPADLLEMNDKVFPLRNKEAGRYPSSALLGRQ
  FSHTTAALEEVSRCVNDMHNFTNEILTWDLDPQAKQQADKSPCTTESTGLDRSEHRSEIPVINLKTSPQQ
  KMPVCSFNGSTPITRSTGDWTETFTEGKPVKGYLSSTKEAGGKGVPDSSQPISGSGAATVEVTLPDLRGT
  WPNSVLGEVTEVDSSGESDDTVIEDTTENPSVKNNKVLPEKSDSLPSAAVKTSERENKETTSHETVRSEM
  YENSEQQQAHAETPTQRSLEGQMSPQVPNMLNEVIPENLAMTDTTRVCSAAPPSVLSETGFSLTVPASAK
  SESLLGKNVEDTDGSSPEDLMAALTGTEEKGTVDKEKGDVLEAVLEKIANVKNTLPVELLHENKLSSSET
  KNTKSKYSEHSRETNGGAPKVSSDLEQEQLTIRAIKELGAKQVQAERMSPGGKLKRTFDPQSGPQNSSDI
  LEHTDITTGSDLGIPKKPTIIKDTRIDSISSLTKTETVNKHVLARLLSDFPVHDLIFWRDVKKTGFVFGT
  TLIMLLSLAAFSVISVVSYLILALLSVTISFRVYKSVIQAVQKSEEGHPFKAYLDVDITLSSEAFHSYMN
  AAMVHVNKALKLIIRLFLVEDLVDSLKLAVFMWLMTYVGAVFNGITLLILAELLVFSVPIVYEKYKTQID
  HYVGIARDQTKSIVEKIQAKLPGIAKKKAE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080909
• ORF Size: 2820 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_080909.2, NM_080909.3, NP_543185.2
• RefSeq Size: 4926
• RefSeq ORF: 2823
• Locus ID: 140945
• MW: 101.5 kDa
• Gene Summary: This gene encodes a member of the reticulon family, a large group of membrane-bound proteins that primarily localize to the endoplasmic reticulum with a small subpopulation at the cell surface. These proteins play roles in endocytosis, exocytosis, trafficking and neuronal outgrowth. Proteins belonging to this family are characterized by a carboxy-terminal reticulon homology domain that consists of a small hydrophilic loop flanked by hydrophobic regions. In rat, this protein has been shown to interact with two members of the synaptic adhesion-like molecule family, suggesting that it may function in trafficking of these proteins. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]