Sh2b1 (NM_134456) Rat Tagged ORF Clone

CAT#: RR200246

Sh2b1 (Myc-DDK-tagged ORF) - Rat SH2-B PH domain containing signaling mediator 1 (Sh2bpsm1), transcript variant 1, (10 ug)

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sh2b1
• Synonyms: Sh2-b; Sh2b; Sh2bpsm1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200246 representing NM_134456
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGGTGCCCCTTCCCCAGAGGATGGGGTTTTCCCTTCTCCACCAGCGCTGCCACCACCCCCTCCCC
  CAAGTTGGCAAGAGTTCTGTGAGTCCCATGCGAGGGCTGCTGCCCTGGATCTTGCTCGCCGTTTTCGCCT
  CTATCTGGCCTCCCACCCACAATATGCAGAGCCGGGAGCAGAGGCTGCCTTTTCTGGCCGTTTTGCTGAG
  CTCTTCCTGCAGCACTTCGAAGCTGAGGTGGCTCGGGCCTCGGGCTCACTCTCCCCACCTGTCTTGGCTC
  CATTGAGCCCTGGTGTGGAAATCCCACCATCACATGACCTGTCCCTTGAGAGCTGCAGGGTGGGTGGGCC
  CCTGGCAGTGTTGGGCCCTTCTCGATCTTCTGAGGACCTGGCTGGCCCCCTTCCTTCCTCAGTCTCTTCC
  TCTACAACGTCCTCAAAGCCGAAGCTCAAGAAACGCTTCTCCCTCCGCTCGGTGGGTCGTTCAGTCAGAG
  GTTCTGTCCGAGGCATCCTGCAGTGGCGGGGGGCTGTTGAATCTCCCTCCCAAGCTGGGCCTCTGGAGAC
  CACATCAGGTCCTCCAGTTCTAGGTGGAAACAGCAACTCCAACTCCTCTGGTGGTGCTGGGACAGTTGGT
  AGGGCATTGGCCAACGATGGCACATCCCCTGGGGAGAGATGGACTCATCGCTTTGAGAGGCTAAGGCTAA
  GTCGTGGAGGGGGAACCTTGAGAGACGGAGCAGGAGTGATACAGAGAGAAGAGCTGCTGAGTTTCATGGG
  GGCTGAAGAGGCTGCCCCTGACCCAGCAGGAGTAGGTCGTGGAGGAGGGGCAGCTGGGCTGACCTCGGGA
  GGAGGAGGGCAGCCTCAGTGGCAGAAATGTCGATTACTGCTCCGGAGTGAAGGAGAAGGAGGAGGAGGAA
  GTCGCTTGGAGTTCTTTGTACCACCCAAGGCATCCCGGCCCCGTCTTAGCATTCCCTGTTCTACTATTAC
  TGATGTCCGCACAGCCACAGCCCTGGAGATGCCTGACAGGGAGAACACGTTTGTGGTTAAGGTAGAAGGC
  CCTTCAGAGTACATCCTGGAGACAACTGATGCACTTCATGTGAAGGCCTGGGTGTCTGACATCCAAGAGT
  GCCTAAGCCCAGGACCCTGCCCTGCTATCAGCCCCCGTCCCATGACCCTTCCCCTGGCCCCTGGGACCTC
  CTTCCTCACAAAGGATAACACAGAGAGCCTGGAGTTGCCCTGCCTGAATCATTCAGAGAGTCTGCCTAGC
  CAGGATCTTCTTCTGGGACCCAGCGAGAGTAACGACCGCCTGTCGCAGGGAGCTTATGGAGGCCTCTCAG
  ACCGGCCGTCAGCGTCCTTCTCCCCTAGTTCTGCCTCCATTGCTGCTTCCCATTTTGACTCAATGGAACT
  GCTTCCTCCAGAGTTGCCCCCTCGGATTCCCATTGAGGAGGGGCCTCCAGCAGGGACAGTTCATCCCCTC
  TCTACCCCGTACCCTCCCCTGGATACTCCTGAAGCAGCCACAGGGTCATTCCTCTTTCAAGGGGAGGCAG
  AGGGGGGTGAGGGGGACCAGCCCCTCTCAGGCTACCCTTGGTTCCACGGCATGCTCTCTCGGCTCAAAGC
  TGCCCAGTTAGTGTTAGAAGGAGGTACCAGCTCCCATGGTGTCTTCTTGGTACGCCAGAGTGAGACAAGA
  CGTGGTGAATATGTCCTCACTTTCAACTTCCAGGGCAAGGCTAAGCACCTGCGTTTGTCACTAAATGAGG
  AGGGTCAGTGCCGGGTCCAACATCTGTGGTTCCAGTCCATTTTCGATATGCTTGAGCACTTCCGGGTGCA
  CCCCATCCCTCTGGAGTCTGGAGGCTCCAGTGATGTTGTCCTTGTCAGCTATGTGCCCTCCCAGCGGCAG
  CAGGAACGGAGCACCTCCCGTGATCCAACCCAGCCCTCTGAACCCCCTCCATGGACAGATCCCCCACATC
  CTGGGGCAGAAGAGGCGTCGGGGGCGCCAGAAGTGGCGGCAGCCACAGCCGCAGCAGCCAAAGAGAGGCA
  AGAGAAGGAGAAAGCGGGCGGCGGAGGGGTCCAGGAAGAGCTGGTCCCCATGGCTGAGCTGGTCCCCATG
  GCTGAATTGGAAGAGGCCATAGCACCAGGCACTGAGGCTCAGGGTGGTGCTGGCTCTAGTGGGGACTTGG
  AGGTGTCCCTAATGGTTCAGCTCCAGCAGTTACCACTAGGGGGCAACGGAGAAGAAGGGGGTCACCCCCG
  AGCCATTAATAACCAGTACTCATTTGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200246 representing NM_134456
  Red=Cloning site Green=Tags(s)
  
  MNGAPSPEDGVFPSPPALPPPPPPSWQEFCESHARAAALDLARRFRLYLASHPQYAEPGAEAAFSGRFAE
  LFLQHFEAEVARASGSLSPPVLAPLSPGVEIPPSHDLSLESCRVGGPLAVLGPSRSSEDLAGPLPSSVSS
  STTSSKPKLKKRFSLRSVGRSVRGSVRGILQWRGAVESPSQAGPLETTSGPPVLGGNSNSNSSGGAGTVG
  RALANDGTSPGERWTHRFERLRLSRGGGTLRDGAGVIQREELLSFMGAEEAAPDPAGVGRGGGAAGLTSG
  GGGQPQWQKCRLLLRSEGEGGGGSRLEFFVPPKASRPRLSIPCSTITDVRTATALEMPDRENTFVVKVEG
  PSEYILETTDALHVKAWVSDIQECLSPGPCPAISPRPMTLPLAPGTSFLTKDNTESLELPCLNHSESLPS
  QDLLLGPSESNDRLSQGAYGGLSDRPSASFSPSSASIAASHFDSMELLPPELPPRIPIEEGPPAGTVHPL
  STPYPPLDTPEAATGSFLFQGEAEGGEGDQPLSGYPWFHGMLSRLKAAQLVLEGGTSSHGVFLVRQSETR
  RGEYVLTFNFQGKAKHLRLSLNEEGQCRVQHLWFQSIFDMLEHFRVHPIPLESGGSSDVVLVSYVPSQRQ
  QERSTSRDPTQPSEPPPWTDPPHPGAEEASGAPEVAAATAAAAKERQEKEKAGGGGVQEELVPMAELVPM
  AELEEAIAPGTEAQGGAGSSGDLEVSLMVQLQQLPLGGNGEEGGHPRAINNQYSFV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134456
• ORF Size: 2268 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_134456.2, NM_134456.3, NP_604451.2
• RefSeq Size: 2991
• RefSeq ORF: 2271
• Locus ID: 89817
• MW: 79.6 kDa
• Gene Summary: interacts with kinase-active tyrosyl-phosphorylated JAK2 [RGD, Feb 2006]